Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Host organism: Eucommia ulmoides
Organ / tissue: bark
NCBI PubMed ID: 25805340Publication DOI: 10.1007/s00253-015-6491-7Journal NLM ID: 8406612Publisher: Springer
Correspondence: Shi J <sjlshi2004
aliyun.com>
Institutions: College of Food Science and Engineering, Northwest A&F University, Yangling, China, College of Enology, Northwest A&F University, Yangling, China, Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an, China
Phomopsis sp. XP-8 is an endophytic fungus that has the ability to produce pinoresinol diglucoside (PDG) in vitro and thus has potential application for the biosynthesis of PDG independent of plants. When cultivated in mung bean medium, PDG production was significantly improved and pinoresinol monoglucoside (PMG) and pinoresinol (Pin) were also found in the culture medium. In this experiment, starch, protein, and polysaccharides were isolated from mung beans and separately used as the sole substrate in order to explore the mechanism of fermentation and identify the major substrates that attributed to the biotransformation of PDG, PMG, and Pin. The production of PDG, PMG, and Pin was monitored using high-performance liquid chromatography (HPLC) and confirmed using HPLC-MS. Activities of related enzymes, including phenylalanine ammonia-lyase (PAL), trans-cinnamate 4-hydroxylase (C4H), and 4-coumarate-CoA ligase (4CL) were analyzed and tracked during the cultivation. The reaction system contained the compounds isolated from mung bean in the designed amount. Accumulation of phenylalanine, cinnamic acid, p-coumaric acid, PDG, PMG, and Pin and the activities of PAL, C4H, and 4CL were measured during the bioconversion. PMG was found only when mung bean polysaccharide was analyzed, while production of PDG and Pin were found when both polysaccharide and starch were analyzed. After examining the monosaccharide composition of the mung bean polysaccharide and the effect of the different monosaccharides had on the production of PMG, PDG, and Pin, galactose in mung bean polysaccharide proved to be the major factor that stimulates the production of PMG
bioconversion, pinoresinol, pinoresinol diglucoside, Phomopsis sp. XP-8, pinoresinol monoglucoside
Structure type: monomer
Location inside paper: abstract
Trivial name: pinoresinol monoglucoside
Compound class: glycoside
Contained glycoepitopes: IEDB_142488,IEDB_146664,IEDB_983931,SB_192
Methods: TLC, ESI-MS, HPLC, UV, cell growth, enzymatic assay, spectrophotometry, Bradford method, evaporation, centrifugation
Enzymes that release or process the structure: PAL (phenylalanine ammonia lyase), TAL (tyrosine ammonia lyase), C4H (trans-cinnamate 4-hydroxylase), 4CL (4-coumaroyl-CoA ligase)
Synthetic data: enzymatic in vivo
Related record ID(s): 48009, 60802, 120659
NCBI Taxonomy refs (TaxIDs): 1961671
Show glycosyltransferases
There is only one chemically distinct structure:
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