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1. (Article ID: 4801)
Shashkov AS, Kenyon JJ, Arbatsky NP, Shneider MM, Popova AV, Miroshnikov KA, Volozhantsev NV, Knirel YA
Structures of three different neutral polysaccharides of Acinetobacter baumannii, NIPH190, NIPH201, and NIPH615 assigned to K30, K45, and K48 capsule types, respectively, based on capsule biosynthesis gene clusters
Carbohydrate Research 417 (2015)
81-88
Neutral capsular polysaccharides (CPSs) were isolated from Acinetobacter baumannii NIPH190, NIPH201, and NIPH615. The CPSs were found to contain common monosaccharides only and to be branched with a side-chain 1→3-linked β-d-glucopyranose residue. Structures of the oligosaccharide repeat units (K units) of the CPSs were elucidated by 1D and 2D 1H and 13C NMR spectroscopy. Novel CPS biosynthesis gene clusters, designated KL30, KL45, and KL48, were found at the K locus in the genome sequences of NIPH190, NIPH201, and NIPH615, respectively. The genetic content of each gene cluster correlated with the structure of the CPS unit established, and therefore, the capsular types of the strains studied were designated as K30, K45, and K48, respectively. The initiating sugar of each K unit was predicted, and glycosyltransferases encoded by each gene cluster were assigned to the formation of the linkages between sugars in the corresponding K unit.
Acinetobacter baumannii, capsular polysaccharide structure, glycosyltransferase, K locus, polysaccharide gene cluster
NCBI PubMed ID: 26432612Publication DOI: 10.1016/j.carres.2015.09.004Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Y.A. Knirel
gmail.com>
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, M. M. Shemyakin & Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia, State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia, School of Molecular Bioscience, The University of Sydney, Sydney, Australia, School of Biomedical Sciences, Queensland University of Technology, Brisbane, Australia, Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russia
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, GLC, de-O-acetylation, NMR-1D, GPC, extraction, bioinformatic analysis
The publication contains the following compound(s):
- Compound ID: 12028
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b-D-Glcp-(1-3)-+
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-2)-a-D-Galp-(1-6)-a-D-Glcp-(1-4)-a-D-GalpNAc-(1-3)-b-D-GalpNAc-(1- |
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Structure type: polymer chemical repeating unit
Compound class: CPS
Reference(s) to other database(s): GTC:G68883RH
- Compound ID: 12029
|
b-D-Glcp-(1-3)-+
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-3)-a-D-Glcp-(1-6)-a-D-GlcpNAc-(1-4)-a-D-Galp-(1-3)-b-D-GlcpNAc-(1- |
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Structure type: polymer chemical repeating unit
Compound class: CPS
Reference(s) to other database(s): GTC:G35428RP
- Compound ID: 12030
|
b-D-Glcp-(1-3)-+
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-4)-a-D-GalpNAc-(1-4)-a-D-GalpNAc-(1-3)-a-D-GlcpNAc-(1- |
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Structure type: polymer chemical repeating unit
Compound class: CPS
Reference(s) to other database(s): GTC:G69532WB
- Compound ID: 12031
|
b-D-Glcp-(1-3)-+
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-4)-a-D-GalpNAc6(50%)Ac-(1-4)-a-D-GalpNAc-(1-3)-a-D-GlcpNAc-(1- |
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Structure type: polymer chemical repeating unit
Compound class: CPS
Reference(s) to other database(s): GTC:G57327XB
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