Show legend Show as text

Structure type: oligomer
Compound class: O-polysaccharide, O-antigen

• Article ID: 1831
  Knirel YA, Vinogradov EV, Shashkov AS, Dmitriev BA, Kochetkov NK "Structure of Pseudomonas aeruginosa O13 O-specific polysaccharide containing 5,7-diacetamido-3,5,7,9-tetradeoxy-D-glycero-L-galacto-nonulosonic acid and 2-acetamidino-2,6-dideoxy-L-galactose" - Bioorganicheskaya Khimia = Bioorganic Chemistry [Russian] 12 (1986) 848-851
  

  O-Specific polysaccharide chain of P. aeruginosa 013 (Lányi) lipopolysaccharide is composed of N-acetyl-D-quinovosamine (QuiNAc), acetamidino derivative of L-fucosamine (FucNAm), and 5,7-diacetamido-3,5,7,9-tetradeoxy-D-glycero-L-galacto-nonulosonic acid (Sug). On solvolysis with HF in methanol, the polysaccharide afforded methylglycosides of a disaccharide and a trisaccharide both containing fucosamine and ulosonic acid derivatives. Chemical transformations (alkaline hydrolysis, reductive deamination, acetylation accompanied by intramolecular acylation of acetamidino group by ulosonic acid), 1H and 13C NMR analysis and mass spectral data proved the following structure of the trisaccharide unit of the polysaccharide: -8)-β-Sug-(1-3)-α-L-FucNAm-(1-3)-α-D-QuiNAc -(1-

  NCBI PubMed ID: 2430578
  Journal NLM ID: 7804941
  Publisher: Moskva: Nauka
  Institutions: N.D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the USSR, Moscow, Russia
  Methods: 13C NMR
  
   
  
  Pseudomonas aeruginosa 13, Pseudomonas aeruginosa Lanyi
  CSDB ID 110525 (all data & tools)
• Article ID: 2213
  Vinogradov EV, Shashkov AS, Knirel YA, Kochetkov NK, Dabrowski J, Grosskurth H, Stanislavsky ES, Kholodkova EV "The structure of the O-specific polysaccharide chain of the lipopolysaccharide of Salmonella arizonae O61" - Carbohydrate Research 231 (1992) 1-11
  

  The O-specific polysaccharide was obtained by mild degradation of the Salmonella arizonae O61 lipopolysaccharide with acid. It contained 2-acetamido-2-deoxy-D-glucose, 2-acetamidino-2,6-dideoxy-L-galactose (FucAm), and 7-acetamido-3,5,7,9-tetradeoxy-5-[(R)-3-hydroxybutyramido]-D- glycero-L-galacto-nonulosonic acid (Sug). On the basis of partial acid hydrolysis with 0.1 M HCl, solvolysis with anhydrous HF in methanol, and 1H- and 13C-NMR analysis (including 1H/13C inversely correlated spectroscopy for localisation of N-acyl substituents), it was concluded that the O-specific polysaccharide had the following structure. →3)-α-L-FucAm-(1→3)-α-D-GlcNAc-(1→8)-β-Sug-(2→. The O-antigen of S. arizonae O61 is structurally related to that of Pseudomonas aeruginosa O12, thus explaining the known serological cross-reactivity between these micro-organisms.

  NCBI PubMed ID: 1394306
  Publication DOI: 10.1016/0008-6215(92)84002-A
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Max Planck Institute for Medical Research, Heidelberg Germany, I.I. Mechnikov Institute of Vaccines and Sera, Moscow Russia
  Methods: 13C NMR, 1H NMR, NMR-2D, partial acid hydrolysis, HF solvolysis, sugar analysis, acid hydrolysis, GPC
  
   
  
  Pseudomonas aeruginosa O12
  CSDB ID 2668 (all data & tools)