Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Acinetobacter baumannii [ICD11:
XN8LS 
]
The structure was elucidated in this paperNCBI PubMed ID: 9232839Journal NLM ID: 0043535Publisher: Elsevier
Institutions: School of Chemistry, University of Hull, UK.
The polymeric O antigen was obtained from lipopolysaccharide extracted from isolated, defatted cell walls of the reference strain for Acinetobacter baumannii serogroup O18. Monosaccharide analyses and NMR spectra established that the polymer had a regular structure with a repeating unit based on residues of D-galactose (2), N-acetyl-D-galactosamine (1), and N-acetyl-D-mannosamine (1). Further interpretation of the NMR spectra, combined with the results of methylation analysis and a Smith degradation, showed that the repeating unit had the following structure. β-D-ManpNAc-(1→4)-α-D-Galp 1 decreases 4 →3)-β-D-GalpNAc-(1→3)-β-D-Galp-(1→.
Lipopolysaccharide, antigen, LPS, structure, Acinetobacter, Acinetobacter baumannii, O18 antigen
Structure type: polymer chemical repeating unit
Location inside paper: Abstract, structure 1
Compound class: O-polysaccharide, O-antigen, CPS
Contained glycoepitopes: IEDB_130648,IEDB_130651,IEDB_134627,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_144987,IEDB_147450,IEDB_151528,IEDB_190606,IEDB_742247,IEDB_885813,SB_165,SB_166,SB_187,SB_195,SB_21,SB_23,SB_24,SB_31,SB_62,SB_7,SB_8,SB_88
Methods: methylation, NMR, Smith degradation
NCBI Taxonomy refs (TaxIDs): 470Reference(s) to other database(s): GTC:G40148IO, GlycomeDB:
25205
Show glycosyltransferases
NMR conditions: in D2O at 343 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,2 Ac
3 bDGalpN 103.58 52.43 80.24 69.12 75.49 61.68
4,4,2 Ac
4,4 bDManpN 100.99 54.14 72.83 68.03 77.05 61.07
4 aDGalp 99.48 69.56 70.17 77.80 70.69 61.49
bDGalp 105.40 71.83 80.24 74.83 76.36 61.92
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,2 Ac
3 bDGalpN 4.74 4.05 3.88 4.07 3.63 ?
4,4,2 Ac
4,4 bDManpN 4.91 4.49 3.79 3.50 3.39 3.79-3.90
4 aDGalp 4.91 3.74 3.90 4.21 4.51 3.76-3.82
bDGalp 4.46 3.61 3.78 4.19 3.68 ?
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,2 Ac
3 bDGalpN 103.58/4.74 52.43/4.05 80.24/3.88 69.12/4.07 75.49/3.63 61.68/?
4,4,2 Ac
4,4 bDManpN 100.99/4.91 54.14/4.49 72.83/3.79 68.03/3.50 77.05/3.39 61.07/3.79-3.90
4 aDGalp 99.48/4.91 69.56/3.74 70.17/3.90 77.80/4.21 70.69/4.51 61.49/3.76-3.82
bDGalp 105.40/4.46 71.83/3.61 80.24/3.78 74.83/4.19 76.36/3.68 61.92/?
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,2 | Ac | |
| 3 | bDGalpN | 4.74 | 4.05 | 3.88 | 4.07 | 3.63 | ? |
| 4,4,2 | Ac | |
| 4,4 | bDManpN | 4.91 | 4.49 | 3.79 | 3.50 | 3.39 | 3.79
3.90 |
| 4 | aDGalp | 4.91 | 3.74 | 3.90 | 4.21 | 4.51 | 3.76
3.82 |
| | bDGalp | 4.46 | 3.61 | 3.78 | 4.19 | 3.68 | ? |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,2 | Ac | |
| 3 | bDGalpN | 103.58 | 52.43 | 80.24 | 69.12 | 75.49 | 61.68 |
| 4,4,2 | Ac | |
| 4,4 | bDManpN | 100.99 | 54.14 | 72.83 | 68.03 | 77.05 | 61.07 |
| 4 | aDGalp | 99.48 | 69.56 | 70.17 | 77.80 | 70.69 | 61.49 |
| | bDGalp | 105.40 | 71.83 | 80.24 | 74.83 | 76.36 | 61.92 |
|
There is only one chemically distinct structure:
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