Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
NCBI PubMed ID: 25965498Publication DOI: 10.1016/j.carbpol.2015.03.045Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: mcunha
utfpr.edu.br
Institutions: Department of Chemistry, Federal Technological University of Parana, Pato Branco, Brazil, Biorefining and Biotechnology Consultancy, Londrina, Brazil, Midwestern State University (Graduate Program in Pharmaceutical Sciences), CEDETEG Campus, Guarapuava, Brazil, Department of Chemistry—CCE, Londrina State University, Londrina, Brazil, Chemistry Department, Federal University of Paraná, Curitiba, Brazila
D-Glucans possess immunomodulatory activities and potential for the development of new therapeutic agents. Biological activities can be enhanced in these biopolymers through chemical derivatization, e.g., carboxymethylation. This work presents the carboxymethylation, characterization and the evaluation of antioxidant activities of the exocellular (1→6)-β-D-glucan produced by Lasiodiplodia theobromae MMPI. Thermal analysis indicated that the native and carboxymethylated polysaccharides presented four stages of mass-loss. The first stage occurred at 125°C (loss of water) with two consecutive events of mass loss (200-400°C) attributed to polymer degradation and the fourth stage between 425 and 620°C (final decomposition). Scanning electron microscopy analysis indicated that the gross morphological features of lasiodiplodan were ruptured following carboxymethylation. X-ray diffractometry analysis demonstrated that the native and carboxymethylated polysaccharides presented a non-crystalline structure. Carboxymethylation contributed to improving the polysaccharide's water solubility and antioxidant capacity
exopolysaccharides, β-Glucans, Biopolymers, antioxidant capacity
Structure type: homopolymer
Location inside paper: abstract, Fig. 2, top spectrum
Trivial name: pustulan, β-1,6-glucan, β-1,6-D-glucan, β(1-6)-D-glucan, β-(1,6)-glucan, lasiodiplodan, pustulan, β-(1,6)-glucan, lasiodiplodan, β-(1,6)-glucan, β-(1,6)-glucan, lasiodiplodan, pustulan, β-1,6-glucan, β-(1,6)-glucan, pustulan, β-(1→6)-glucan PCPS, water-soluble glucan (PS-I)
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glycoprotein, glucan, polysaccharide, cell wall glucoprotein
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: 13C NMR, IR, X-ray, biological assays, cell growth, dialysis, antioxidant activities, precipitation, SEM, derivatization, evaporation, centrifugation, thermal analysis, ion exchange, titration
Biological activity: lasiodiplodan exhibits scavenging activity of DPPH-radical and ABTS-radical, and ferric ion reducing ability
Related record ID(s): 7724, 8325, 42140, 43301, 43379, 44888, 44927, 44939, 45578, 47842, 47843, 48303, 48367, 48412, 48423, 48443, 49291, 101372, 124916, 138263, 143680, 143682, 149870
NCBI Taxonomy refs (TaxIDs): 45133Reference(s) to other database(s): GTC:G26777BZ, GlycomeDB:
863, CCSD:
50854, CBank-STR:4234
Show glycosyltransferases
NMR conditions: in D2O at 303(C) K
[as TSV]
13C NMR data: present in publication
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There is only one chemically distinct structure:
Found 
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