Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperPublication DOI: 10.4314/tjpr.v14i3.19Journal NLM ID: 101214391Publisher: Benin City, Nigeria: Faculty of Pharmacy, University of Benin
Correspondence: Li HB <hongbo715
163.com>
Institutions: School of Food Science, Henan Institute of Science and Technology, Xinxiang, China, School of Marine Sciences, Ningbo University, Ningbo, China
The structures of cellulose-extract derivative (CED) formed by heating Lentinus edodes cellulose in water surroundings that can efficiently inhibit aflatoxin production by Aspergillus flavus were identified. CED was purified on Sepharose CL-6B columns, and then structurally characterized using amino acid analyzer, gas chromatography, Fourier transform infrared spectroscopy, and polyacrylamide gel electrophoresis. Results: CED completely inhibited aflatoxin AFB1 production by A. flavus at concentrations ≥ 100 μg/ mL. Chemical analysis indicated that CED contained 82% carbohydrate and 18% protein and has a molecular weight of approximately 24 kDa. Monosaccharide component analysis indicates that glucose was the predominant monosaccharide of CED. Analysis by Smith degradation and enzymatic hydrolysis indicate that there were only (1,4)-glycosidic linkages in the CED polysaccharide chains. The protein backbone of CED contained 15 kinds of amino acid with higher levels of glutamic acid, aspartic acid, leucine and alanine. CED was identified as a complex of peptide and polysaccharide structures possessing β-(1,4)-glucan backbones, and it provides a theoretical basis for developing polysaccharide preparations to control aflatoxin contamination with medical and food science applications
amino acid, Lentinus edodes, Aspergillus flavus, structure identification, aflatoxin B1, cellulose derivative
Structure type: homopolymer ; 24000
Location inside paper: p. 499, left column, paragraph 2, 3
Aglycon: protein
Trivial name: cellulose
Compound class: polysaccharide, β-(1, 4)-glucan
Contained glycoepitopes: IEDB_142488,IEDB_146664,IEDB_983931,SB_192
Methods: IR, GC-MS, acid hydrolysis, GC, Smith degradation, amino acid analysis, electrophoresis, HPLC, enzymatic digestion, extraction, periodate oxidation, acetylation, reduction, cell growth, precipitation, phenol-sulfuric acid assay, spectrophotometry, Bradford method, evaporation
Biological activity: the cellulose derivative in concentration ≥ 100 μg/mL can completely inhibit aflatoxin production by A. flavus
Enzymes that release or process the structure: proteinase K, cellulase
NCBI Taxonomy refs (TaxIDs): 5353Reference(s) to other database(s): GTC:G75830OM
Show glycosyltransferases
There is only one chemically distinct structure:
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(later renamed to: Lentinula edodes)