Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
The structure was elucidated in this paperNCBI PubMed ID: 23411445Publication DOI: 10.1016/j.biortech.2012.12.075Journal NLM ID: 9889523Publisher: Elsevier
Correspondence: Mao W <wenjunmqd
hotmail.com>
Institutions: Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, Qingdao, China, College of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, China
The deep-sea fungus Penicillium griseofulvum produces an extracellular polysaccharide, Ps1-1, when grown in potato dextrose-agar medium. Ps1-1 was isolated from the fermented broth using ethanol precipitation, anion-exchange and size-exclusion chromatography. Ps1-1 is a galactomannan with a molecular weight of about 20 kDa, and a molar ratio of mannose and glucose of 1.1:1.0. On the basis of one- and two-dimensional nuclear magnetic resonance (1D and 2D NMR) and mass spectroscopic analyses, Ps1-1 is composed of a long chain of galactofuranan and a mannose core. The galactofuranan chain consists of (1→5)-linked β-galactofuranose, with additional branches at C-6 consisting of (1→)-linked β-galactofuranose residues and phosphate esters. The mannan core is composed of (1→6)-linked α-mannopyranose substituted at C-2 by (1→)-linked α-mannopyranose residues, disaccharide and trisaccharide units of (1→2)-linked α-mannopyranose. The investigation demonstrated that Ps1-1 was a galactofuranose-containing galactomannan differing from previously described extracellular polysaccharides.
characterization, extracellular polysaccharide, preparation, deep-sea fungus, Penicillium griseofulvum
Structure type: fragment of a bigger structure
Location inside paper: p. 181, column 1, paragraph 4, Ps1-1
Compound class: EPS, galactofuranan
Contained glycoepitopes: IEDB_136095,IEDB_137472,IEDB_149137,IEDB_149176,IEDB_190606,IEDB_885812
Methods: 13C NMR, 1H NMR, IR, acetolysis, partial hydrolysis, RP-HPLC, dialysis, HPGPC, phenol-sulfuric acid assay, HMBC, HMQC, COSY, methylation assay, Lowry method, centrifugaiton, ion-exhange chromatography
Comments, role: long chain attached to mannan core; total mass of ID 48503 and 48504 is 20000 Da; D configuration of Galp residues was assumed by CSDB staff; overall percetage of branched residues is 20 %
Related record ID(s): 44840, 48504
NCBI Taxonomy refs (TaxIDs): 5078
Show glycosyltransferases
NMR conditions: in D2O at 296 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
5 bDGalf 108.4 83.8 77.5 82.5 76.5 68.0
bDGalf 108.2 82.5 77.5 82.5 76.5 60.5
5,6 bDGalf 109.0 82.5 77.5 82.5 70.4 60.5
5,6 P
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
5 bDGalf 5.21 7.16 4.12 4.17 3.89 3.69
bDGalf 5.22 4.18 4.13 4.17 3.89 3.71-3.84
5,6 bDGalf 5.25 4.17 4.13 4.17 3.96 3.80
5,6 P
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
5 bDGalf 108.4/5.21 83.8/7.16 77.5/4.12 82.5/4.17 76.5/3.89 68.0/3.69
bDGalf 108.2/5.22 82.5/4.18 77.5/4.13 82.5/4.17 76.5/3.89 60.5/3.71-3.84
5,6 bDGalf 109.0/5.25 82.5/4.17 77.5/4.13 82.5/4.17 70.4/3.96 60.5/3.80
5,6 P
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 5 | bDGalf | 5.21 | 7.16 | 4.12 | 4.17 | 3.89 | 3.69 |
| | bDGalf | 5.22 | 4.18 | 4.13 | 4.17 | 3.89 | 3.71
3.84 |
| 5,6 | bDGalf | 5.25 | 4.17 | 4.13 | 4.17 | 3.96 | 3.80 |
| 5,6 | P | |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 5 | bDGalf | 108.4 | 83.8 | 77.5 | 82.5 | 76.5 | 68.0 |
| | bDGalf | 108.2 | 82.5 | 77.5 | 82.5 | 76.5 | 60.5 |
| 5,6 | bDGalf | 109.0 | 82.5 | 77.5 | 82.5 | 70.4 | 60.5 |
| 5,6 | P | |
|
There is only one chemically distinct structure:
Found 
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