Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wall
The structure was elucidated in this paperNCBI PubMed ID: 29885339Publication DOI: 10.1016/j.jbiotec.2018.06.305Journal NLM ID: 8411927Publisher: Amsterdam: Elsevier
Correspondence: anna_bzducha_wrobel
sggw.pl
Institutions: Faculty of Food Science, Department of Biotechnology, Microbiology and Food Evaluation, Warsaw University of Life Sciences-SGGW, Warszawa, Poland, Faculty of Food Science, Department of Food Engineering and Process Management, Warsaw University of Life Sciences-SGGW, Warszawa, Poland
Changes in cell wall structure of four strains of Sacccharomyces cerevisiae species (brewer's, baker's and probiotic yeast) after culturing on deproteinated potato juice water (DPJW) with diverse addition of glycerol and different pH were investigated. It allowed to select conditions intensifying biosynthesis of β(1,3)/(1,6)-glucan and mannoproteins of cell walls of tested strains. Yeast cell wall structural polysaccharides show biological activity and technological usability in food industry but also decide about therapeutic properties of yeast biomass. The highest increase in the thickness of walls (by about 100%) and β-glucan layer (by about 120%) was stated after cultivation of S. cerevisiae R9 brewer's yeast in DPJW supplemented with 5 and 10% (w/v) of glycerol and pH 7.0 while S. cerevisiae var. boulardi PAN yeast synthesized by ab. 70% thicker β-glucan layer when the pH of growth medium was equal to 5.0. The cells of brewer's yeast (S. cerevisiae R9), probiotic (S. cerevisiae CNCM 1-745) and baker's (S. cerevisiae 102) intensified the ratio of mannoproteins in the structure of cell walls cultivated in mediums supplemented with above 15% of glycerol what point out the protective action of glycoprotein's under osmotic stress conditions. The study confirms at the first time the possibility of using agro-industrial waste in biosynthesis of functional polysaccharides of S. cerevisiae cell wall. It could be an new advantage in production of yeast biomass with therapeutic properties or β-glucan preparation as a novel food ingredient.
glycerol, Saccharomyces cerevisiae, mannoproteins, β(1, waste potato juice water, 3)/(1, 6)-glucan
Structure type: structural motif or average structure
Location inside paper: Introduction, paragraph 1
Trivial name: β-1,3-glucan
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: colorimetry, microscopy, TEM, centrifugation, sulfuric acid hydrolysis, ANOVA
Biological activity: rigid structural skeleton of the cell wall; responses osmotic stability and protects against adverse outer conditions to the cell; energy reserve in case of starvation stress
Enzymes that release or process the structure: β(1,3)-glucan synthase, endo-β(1,3)-glucanase, exo-β(1,3)-glucanase
Related record ID(s): 48554
NCBI Taxonomy refs (TaxIDs): 252598,
4932Reference(s) to other database(s): GTC:G51056AN
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There is only one chemically distinct structure:
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(later renamed to: Saccharomyces sp. 'boulardii' PAN)