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Taxonomic group: fungi / Ascomycota (Phylum: Ascomycota)

The structure was elucidated in this paper
NCBI PubMed ID: 29466327
Publication DOI: 10.1038/nature25755
Journal NLM ID: 0410462
Publisher: Basingstoke: Nature Publishing Group
Correspondence: Li H <huilin.li

vai.org>
Institutions: Center for Epigenetics, Van Andel Research Institute, Grand Rapids, USA, Advanced Science Research Center at the Graduate Center of the City University of New York, New York, NY, USA, David Van Andel Advanced Cryo-Electron Microscopy Suite, Van Andel Research Institute, Grand Rapids, USA

N-glycosylation is a ubiquitous modification of eukaryotic secretory and membrane-bound proteins; about 90% of glycoproteins are N-glycosylated. The reaction is catalysed by an eight-protein oligosaccharyltransferase (OST) complex that is embedded in the endoplasmic reticulum membrane. Our understanding of eukaryotic protein N-glycosylation has been limited owing to the lack of high-resolution structures. Here we report a 3.5 Å resolution cryo-electron microscopy structure of the Saccharomyces cerevisiae OST complex, revealing the structures of subunits Ost1-Ost5, Stt3, Wbp1 and Swp1. We found that seven phospholipids mediate many of the inter-subunit interactions, and an Stt3 N-glycan mediates interactions with Wbp1 and Swp1 in the lumen. Ost3 was found to mediate the OST-Sec61 translocon interface, funnelling the acceptor peptide towards the OST catalytic site as the nascent peptide emerges from the translocon. The structure provides insights into co-translational protein N-glycosylation, and may facilitate the development of small-molecule inhibitors that target this process.

structure, oligosaccharyltransferase, Saccharomyces cerevisiae, cryo-electron microscopy, N-glycosilation

Structure type: oligomer
Location inside paper: Fig.2(d)
Aglycon: (->4) Asn-X-Ser/Thr (Stt3p)
Compound class: N-glycan
Contained glycoepitopes: IEDB_130701,IEDB_135813,IEDB_136104,IEDB_137340,IEDB_137485,IEDB_140116,IEDB_141793,IEDB_141795,IEDB_141807,IEDB_141830,IEDB_143632,IEDB_144983,IEDB_151531,IEDB_152206,IEDB_153212,IEDB_983930,SB_136,SB_196,SB_198,SB_44,SB_67,SB_72,SB_74,SB_85

Methods: cryo-EM
Biosynthesis and genetic data: genetic data
Comments, role: Stt3p - subunit of OST; N-glycan moiety attached to Asn539
3D data: conformation data

Related record ID(s): 48607, 48608
NCBI Taxonomy refs (TaxIDs): 4932
Reference(s) to other database(s): GTC:G89806WY
Show glycosyltransferases

There is only one chemically distinct structure:

CC(=O)N[C@H]1[C@H](O[C@@H]2[C@@H](CO)O[C@@H](O)[C@H](NC(C)=O)[C@H]2O)O[C@H](CO)[C@@H](O[C@@H]2O[C@H](CO[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]3O)[C@@H](O)[C@H](O)[C@@H]2O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]2O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]2O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]2O)[C@@H]1O

SVG MW SMILES 3D mol Ionize

structure, oligosaccharyltransferase, Saccharomyces cerevisiae, cryo-electron microscopy, N-glycosilation

Structure type: oligomer
Location inside paper: Extended Data Fig.3(bottom right)
Aglycon: (->4) Asn-X-Ser/Thr (Ost1p)
Compound class: N-glycan
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_137485,IEDB_141807,IEDB_144983,IEDB_151531,IEDB_152206,IEDB_153212,IEDB_983930,SB_44,SB_72,SB_74,SB_85

Methods: cryo-EM
Biosynthesis and genetic data: genetic data
Comments, role: Ost1p - subunit of OST; N-glycan moiety attached to Asn336
3D data: conformation data

Related record ID(s): 48606, 48608
NCBI Taxonomy refs (TaxIDs): 4932
Reference(s) to other database(s): GTC:G15407YE
Show glycosyltransferases

There is only one chemically distinct structure:

CC(=O)N[C@H]1[C@H](O[C@@H]2[C@@H](CO)O[C@@H](O)[C@H](NC(C)=O)[C@H]2O)O[C@H](CO)[C@@H](O[C@@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]2O)[C@@H]1O

SVG MW SMILES 3D mol Ionize

structure, oligosaccharyltransferase, Saccharomyces cerevisiae, cryo-electron microscopy, N-glycosilation

Structure type: monomer
Location inside paper: Extended Data Fig.3(bottom right)
Aglycon: (->4) Asn-X-Ser/Thr (Wbp1p)
Compound class: N-glycan
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_141807,IEDB_151531

Methods: cryo-EM
Biosynthesis and genetic data: genetic data
Comments, role: Wbp1p - subunit of OST; N-glycan moiety attached to Asn60
3D data: conformation data

Related record ID(s): 48606, 48607
NCBI Taxonomy refs (TaxIDs): 4932
Reference(s) to other database(s): GTC:G49108TO
Show glycosyltransferases

There is only one chemically distinct structure:

CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O

SVG MW SMILES 3D mol Ionize