Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
The structure was elucidated in this paperNCBI PubMed ID: 29275441Publication DOI: 10.1007/s00792-017-0984-5Journal NLM ID: 9706854Publisher: Springer
Correspondence: anna.gorbushina
bam.de
Institutions: Department of Biology, Chemistry and Pharmacy, Freie Universitat Berlin, Berlin, Germany, Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, Brazil, Department 4 (Materials and Environment), Bundesanstalt für Materialforschung und -prüfung (BAM), Berlin, Germany, Division 6.6 (Nanotribology and Nano-Structuring), Bundesanstalt für Materialforschung und -prüfung (BAM), Berlin, Germany, Department of Solar Materials, Applied Biocatalytics, Helmholtz Centre for Environmental Research, Permoserstraße, Leipzig, Germany, Institute of Machine Tools and Factory Management, Berlin, Germany, Department of Earth Sciences, Freie Universität Berlin, Berlin, Germany
Melanised cell walls and extracellular polymeric matrices protect rock-inhabiting microcolonial fungi from hostile environmental conditions. How extracellular polymeric substances (EPS) perform this protective role was investigated by following development of the model microcolonial black fungus Knufia petricola A95 grown as a sub-aerial biofilm. Extracellular substances were extracted with NaOH/formaldehyde and the structures of two excreted polymers studied by methylation as well as NMR analyses. The main polysaccharide (~ 80%) was pullulan, also known as α-1,4-; α-1,6-glucan, with different degrees of polymerisation. Αlpha-(1,4)-linked-Glcp and α-(1,6)-linked-Glcp were present in the molar ratios of 2:1. A branched galactofuromannan with an α-(1,2)-linked Manp main chain and a β-(1,6)-linked Galf side chain formed a minor fraction (~ 20%). To further understand the roles of EPS in the weathering of minerals and rocks, viscosity along with corrosive properties were studied using atomic force microscopy (AFM). The kinetic viscosity of extracellular K. petricola A95 polysaccharides (≈ 0.97 × 10-6 m2 s-1) ranged from the equivalent of 2% (w/v) to 5% glycerine, and could thus profoundly affect diffusion-dominated processes. The corrosive nature of rock-inhabiting fungal EPS was also demonstrated by its effects on the aluminium coating of the AFM cantilever and the silicon layer below.
EPS, α-1, pullulan, corrosion, melanised microcolonial fungi (MCF), sub-aerial biofilms (SAB), 4- and α-1, 6-glucans
Structure type: structural motif or average structure
Location inside paper: p. 169, column 2, Fig. 4
Trivial name: pullulan, reuteran, α-glucan
Compound class: EPS
Contained glycoepitopes: IEDB_140629,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_420419,IEDB_420420,IEDB_420421,IEDB_857742,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, methylation, GC-MS, X-ray, FTIR, HPSEC, viscosity measurement, acetylation, NaBH4 reduction, DOSY, phenol-sulfuric acid assay, centrifugation, COSY, HSQC, AFM, light microscopy, TFA hydrolysis, EDX analysis, acetilation
Comments, role: main chain; small percentage of branching at 3 and 6 position; total mass of polysaccharide 17800-117100
Related record ID(s): 48613
NCBI Taxonomy refs (TaxIDs): 206542,
5580Reference(s) to other database(s): GTC:G75629GS
Show glycosyltransferases
NMR conditions: in D2O
[as TSV]
1H NMR data: present in publication
|
There is only one chemically distinct structure:
Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
The structure was elucidated in this paperNCBI PubMed ID: 29275441Publication DOI: 10.1007/s00792-017-0984-5Journal NLM ID: 9706854Publisher: Springer
Correspondence: anna.gorbushina
bam.de
Institutions: Department of Biology, Chemistry and Pharmacy, Freie Universitat Berlin, Berlin, Germany, Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, Brazil, Department 4 (Materials and Environment), Bundesanstalt für Materialforschung und -prüfung (BAM), Berlin, Germany, Division 6.6 (Nanotribology and Nano-Structuring), Bundesanstalt für Materialforschung und -prüfung (BAM), Berlin, Germany, Department of Solar Materials, Applied Biocatalytics, Helmholtz Centre for Environmental Research, Permoserstraße, Leipzig, Germany, Institute of Machine Tools and Factory Management, Berlin, Germany, Department of Earth Sciences, Freie Universität Berlin, Berlin, Germany
Melanised cell walls and extracellular polymeric matrices protect rock-inhabiting microcolonial fungi from hostile environmental conditions. How extracellular polymeric substances (EPS) perform this protective role was investigated by following development of the model microcolonial black fungus Knufia petricola A95 grown as a sub-aerial biofilm. Extracellular substances were extracted with NaOH/formaldehyde and the structures of two excreted polymers studied by methylation as well as NMR analyses. The main polysaccharide (~ 80%) was pullulan, also known as α-1,4-; α-1,6-glucan, with different degrees of polymerisation. Αlpha-(1,4)-linked-Glcp and α-(1,6)-linked-Glcp were present in the molar ratios of 2:1. A branched galactofuromannan with an α-(1,2)-linked Manp main chain and a β-(1,6)-linked Galf side chain formed a minor fraction (~ 20%). To further understand the roles of EPS in the weathering of minerals and rocks, viscosity along with corrosive properties were studied using atomic force microscopy (AFM). The kinetic viscosity of extracellular K. petricola A95 polysaccharides (≈ 0.97 × 10-6 m2 s-1) ranged from the equivalent of 2% (w/v) to 5% glycerine, and could thus profoundly affect diffusion-dominated processes. The corrosive nature of rock-inhabiting fungal EPS was also demonstrated by its effects on the aluminium coating of the AFM cantilever and the silicon layer below.
EPS, α-1, pullulan, corrosion, melanised microcolonial fungi (MCF), sub-aerial biofilms (SAB), 4- and α-1, 6-glucans
Structure type: structural motif or average structure ; 8700-12600
Location inside paper: p. 170, column 2
Compound class: EPS, galactofuromannan
Contained glycoepitopes: IEDB_130701,IEDB_136095,IEDB_136104,IEDB_137472,IEDB_140116,IEDB_141795,IEDB_141830,IEDB_141834,IEDB_143632,IEDB_144983,IEDB_149176,IEDB_152206,IEDB_164480,IEDB_190606,IEDB_76933,IEDB_983930,SB_136,SB_196,SB_44,SB_67,SB_72
Methods: 13C NMR, 1H NMR, methylation, GC-MS, X-ray, FTIR, HPSEC, viscosity measurement, acetylation, NaBH4 reduction, DOSY, phenol-sulfuric acid assay, centrifugation, COSY, HSQC, AFM, light microscopy, TFA hydrolysis, EDX analysis, acetilation
Related record ID(s): 48612
NCBI Taxonomy refs (TaxIDs): 206542,
5584
Show glycosyltransferases
NMR conditions: in D2O
[as TSV]
1H NMR data: present in publication
|
There is only one chemically distinct structure:
Found 
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