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1. (Article ID: 4875)
Arbatsky NP, Shneider MM, Shashkov AS, Popova AV, Miroshnikov KA, Volozhantsev NV, Knirel YA
Structure of the N-acetylpseudaminic acid-containing capsular polysaccharide of Acinetobacter baumannii NIPH67
Russian Chemical Bulletin = Izvestiia Akademii nauk. Seriia khimicheskaia 65(2) (2016)
588-591
Capsular polysaccharide (CPS) was isolated from a nosocomial pathogen Acinetobacter baumannii (A. baumannii) NIPH67 and studied by sugar analysis, Smith degradation, and 1H and 13C NMR spectroscopy. The CPS was found to contain 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-manno-non-2-ulosonic acid (di-N-acetylpseudaminic acid, Pse5Ac7Ac), and the structure of the linear trisaccharide repeating unit of the CPS was established as →4)-α-Psep5Ac7Ac-(2→6)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→. The genetic content of the capsule biosynthesis cluster of A. baumannii NIPH67, designated KL33, is consistent with the established CPS structure, and thus the capsule of the investigated strain was assigned to K33 group. Functions of proteins including two glycosyltransferases encoded by the genes of the K33 locus were assigned based on the structure of CPS and by the comparison with related proteins of other capsular types of A. baumannii.
Acinetobacter baumannii, capsular polysaccharide, pseudaminic acid, glycosyltransferase, biotechnology, K locus, KL33 gene cluster, structure of capsular polysaccharide
Publication DOI: 10.1007/s11172-016-1342-yJournal NLM ID: 100912060Publisher: New York: Consultants Bureau
Correspondence: yknirel
gmail.com
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 47 Leninsky prosp., Moscow, Russian Federation, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 ul. MiklukhoMaklaya, 117997 Moscow, Russian Federation, Moscow Institute of Physics and Technology,9 Institutsky per., 141700 Dolgoprudnyi, Moscow Region, Russian Federation, State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Moscow Region, Russian Federation
Methods: 13C NMR, 1H NMR, NMR-2D, acid hydrolysis, GLC, Smith degradation, composition analysis, mild alkaline degradation, GPC, function analysis of gene clusters
The publication contains the following compound(s):
- Compound ID: 219
Structure type: homopolymer
Trivial name: glucan, α-D-glucan, dextran, α-1,6-glucan, α-(1,6)-glucan, dextran, α-(1,6)-glucan, α-1,6 dextran, β-1,3-D-glucan, polysaccharide IOP
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide
Reference(s) to other database(s): GTC:G69605LY, GlycomeDB:
2461
- Compound ID: 12255
Structure type: polymer chemical repeating unit
Compound class: CPS
Reference(s) to other database(s): GTC:G41500KK
- Compound ID: 12256
Structure type: oligomer
Compound class: CPS
Reference(s) to other database(s): GTC:G40017RD
- Compound ID: 12257
Structure type: oligomer
Compound class: CPS
Reference(s) to other database(s): GTC:G10685AX
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