Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
NCBI PubMed ID: 21810081Publication DOI: 10.1042/BSR20110012Journal NLM ID: 8102797Publisher: London: Portland Press on behalf of the Biochemical Society
Correspondence: Ng TB <b021770
mailserv.cuhk.edu.hk>; Liu F <liufang312
nankai.edu.cn>
Institutions: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China, Key Laboratory of Systems Microbial Biotechnology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China, Department of Microbiology, College of Life Science, Nankai University, Tianjin, China, Research Center of Fishery Production Standard and Quality Chinese Academy of Fishery Sciences, Beijing, China
A novel antioxidant polysaccharide-peptide complex LB-1b from the fruiting bodies of the edible abalone mushroom (Pleurotus abalonus) was purified and identified. The structural characteristic of LB-1b was identified by FTIR (Fourier-transform IR), 13C NMR and 1H NMR spectroscopy. LB-1b is a polysaccharide-peptide complex that contains glucose, rhamnose, glucuronic acid and galactose in the molar ratio of 22.4:1:1.7:1.6 and the N-terminal sequence of its peptide moiety has also been determined. The N-terminal amino acid sequence of LB-1b, IPKERKEFQQAQHLK, showed some resemblance to antioxidant enzymes. LB-1b exhibited high antioxidant activity in erythrocyte haemolysis in vitro and the anti-proliferative activity towards hepatoma HepG2 cells and breast cancer MCF7 cells with an IC50 of 24 and 14 μM respectively. LB-1b also demonstrated hypoglycaemic activity in drug-induced diabetic mice and anti-HIV-1 RT (reverse transcriptase) with an IC50 value of 12.5 μM
biological activity, Structural characterization, Pleurotus abalonus (abalone mushroom), polysaccharide-peptide complex
Structure type: homopolymer ; 16000
Location inside paper: p. 225, left column, paragraph 1
Aglycon: protein
Compound class: glucan, glycopeptide, polysaccharide
Contained glycoepitopes: IEDB_142488,IEDB_146664,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, IR, SDS-PAGE, inhibition studies, acid hydrolysis, amino acid analysis, biological assays, HPLC, FPLC, extraction, CC, precipitation, derivatization, antitumor activity assay, evaporation, anthrone-sulfuric acid assay, amino acid sequence analysis, Lowry method
Biological activity: glucan inhibits the proliferation of MCF7 and HepG2 tumour cells, exhibits anti-HIV-1 RT activity and anti-diabetic activity on drug-induced diabetic mice
NCBI Taxonomy refs (TaxIDs): 47962Reference(s) to other database(s): GTC:G75830OM
Show glycosyltransferases
NMR conditions: in D2O
1H NMR data: present in publication
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13C NMR data: present in publication
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There is only one chemically distinct structure:
Found 
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