Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperNCBI PubMed ID: 236009Publication DOI: 10.1016/0304-4165(75)90363-3Journal NLM ID: 0217513Publisher: Elsevier
Institutions: Department of Microbial Chemistry, Tokyo College of Pharmacy, Shinjuku-ku, Japan
A water-soluble glucuronan "protuberic acid", [α]22D -83.6°, was isolated and purified from Kobayashia Nipponica, and its physicochemical properties were investigated. The purified protuberic acid was homogeneous as shown by zone electrophoresis, gel filtration over Sepharose 4B, and ultracentrifugation. The sedimentation coefficient was 1.8 S and its intrinsic viscosity was 1.1 dl/g. By gel filtration the molecular weight was estimated to be about 170 000. The results of periodate oxidation, methylation analysis, and partial acid hydrolysis indicated that this acidic polysaccharide has a linear structure of mainly 1,4-linkages and containing an acid-labile linkage. Reduced protuberic acid, [α]22D -44°, is also described
glucuronan, Kobayashia Nipponica, protuberic acid
Structure type: homopolymer ; 170000
Location inside paper: abstract
Trivial name: protuberic acid
Compound class: polysaccharide, glucuronan
Contained glycoepitopes: IEDB_115136,IEDB_140630,IEDB_423153
Methods: gel filtration, 1H NMR, methylation, IR, TLC, acid hydrolysis, GLC, Smith degradation, paper chromatography, electrophoresis, viscosity measurement, extraction, periodate oxidation, optical rotation measurement, acetylation, elemental analysis, methylation analysis, reduction, precipitation, centrifugation, phosphate measurement
NCBI Taxonomy refs (TaxIDs): 1419007Reference(s) to other database(s): GTC:G07348VU
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There is only one chemically distinct structure:
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: cell wall
NCBI PubMed ID: 8245838Journal NLM ID: 0375371Institutions: Department of Biochemistry, University of Otago, Dunedin, New Zealand
The cell wall of Trichosporon cutaneum consists of 11% protein, 63% neutral carbohydrate, 9% glucosamine and 13% glucuronic acid. The sugars include glucose (32%), mannose (6%) and traces of xylose and galactose. The cell wall was fractionated with alkali to yield a mixture of alkali-soluble matrix components, and an alkali-insoluble glucan associated with chitin. The alkali-insoluble glucan contained a mixture of (1-3) and (1-6) glycosidic linkages. It was only partly susceptible to digestion by the β(1-3) glucanase, Zymolyase. The alkali-soluble fraction contained glucan, mannan and acidic polymers. The glucan was (1-3)-linked with no (1-6) linkages and only trace amounts of (1-3-6)-linked glucose. It was resistant to digestion by Zymolyase. Extensive hydrolysis of this fraction with trifluoroacetic acid released a high-molecular-mass glucuronan which had 1H- and 13C-NMR profiles matching those of the β(1-4) glucuronan, mucoric acid. Xylomannan was purified from isolated cell walls and from whole cells. It contained glucose, mannose, xylose, and D-glucuronic acid. It was very similar in composition and structure to the capsular polysaccharides of Cryptococcus neoformans, and to an extracellular polysaccharide produced by another yeast described as T. cutaneum. Electron microscopy showed that the cell wall of T. cutaneum has a lamellar structure characteristic of a basidiomycetous yeast rather than the electron-dense 'fuzzy coat' seen in Candida albicans.
Structure type: homopolymer ; n>47
Trivial name: glucuronan
Compound class: glucuronan
Contained glycoepitopes: IEDB_115136,IEDB_140630,IEDB_423153
Methods: 13C NMR, 1H NMR, acid hydrolysis, GLC, electron microscopy, enzymatic digestion, anion exchange chromatography, methylation assay, alkali extraction, alkali extrnction
Comments, role: Apiotrichum curvatum ATCC 20509 is mentioned in the paper as a synonym for Trichosporon cutaneum (see introduction)
Related record ID(s): 139868, 139869, 139871
NCBI Taxonomy refs (TaxIDs): 57679Reference(s) to other database(s): GTC:G07348VU, CCSD:
35175, CBank-STR:5877
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There is only one chemically distinct structure:
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(later renamed to: Protubera nipponica)