Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
NCBI PubMed ID: 19694186Publication DOI: 10.1691/ph.2009.8806Journal NLM ID: 9800766Publisher: Eschborn: Govi-Verlag Pharmazautischer Verlag
Correspondence: Liu JC <qyybliu
126.com>
Institutions: Institute of Medicine, Qiqihar Medical College, Qiqihar, China
LMPAB is a linear β-(1-3)-glucan we isolated from polysaccharide extract of Agaricus blazei Murill (AbM). Effects of LMPAB on splenic natural killer (NK) cell activity, splenocyte proliferation, index of spleen and thymus, IFN-gamma expression in spleen and the concentration of IL-12, IL-18 and TNF-alpha in serum of S180 ascitic tumor-bearing mice were detected. The results showed that intraperitoneal injection of LMPAB (100 mg/kg/d) significantly increased the thymus index. LMPAB augmented splenic NK cell activity in a dose-dependent manner (50-200 mg/kg/d). The concanavalin A (3 microg/ ml) stimulated splenocyte proliferation was significantly enhanced by LMPAB at dosages of 50, 100 or 200 mg/kg/d. Further studies showed that LMPAB (50, 100 or 200 mg/kg/d, 14d) significantly increased the production of IL-12, TNF-alpha, IL-18 and the expression IFN-gamma as determined by ELISA and immunohistochemistry, respectively. These results clearly indicate that the anti-tumor effects of LMPAB are closely associated with up-regulation of activity of NK cells, expression of IFN-gamma in spleen and the systemic level of IL-12, IL-18 and TNF-alpha in tumor-bearing mice
mice, sarcoma 180, Immunostimulatory activity, Agaricus blazei, β-(1-3)-glucan
Structure type: homopolymer ; 48000
Location inside paper: abstract
Trivial name: β-(1,3)-glucan
Compound class: glucan, polysaccharide
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: biological assays, UV, extraction, HPGPC, MTT, immunohistochemistry, immunosorbent assay
Biological activity: glucan is an active polysaccharide that exerts a variety of immunostimulatory activities including up-regulation of activity of NK cells, splenocyte expression of IFN-g and serum levels of IL-12, IL-18 and TNF-a
Related record ID(s): 3459, 42138, 42290, 42294, 43554, 47304, 47707, 48305, 48371, 48410, 48411, 48472, 49256, 49257, 49266, 49282, 49290, 102752
NCBI Taxonomy refs (TaxIDs): 79798Reference(s) to other database(s): GTC:G51056AN
Show glycosyltransferases
There is only one chemically distinct structure:
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