Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
NCBI PubMed ID: 21090112Journal NLM ID: 9426463Publisher: Beijing: Ke xue chu ban she
Correspondence: Liu B <fzliubo
163.com>
Institutions: Agricultural Bioresources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, China
The paper dealt with the characterization of polysaccharide of Paecilomyces lilacinus NH-PL-03 strain. First, we extracted and purified exude polysaccharide from the fungal fermentation broth by ethanol depositing method. Second, the proteins were removed by the Sevage method from the crude polysaccharide. Third, the purified polysaccharide (EP-1) was obtained after Superdex G-75 column separation. The results of UV-spectrometer and Sephacryl S-200 HR chromatography experiments showed that the EP-1 was a homogeneous pure polysaccharide with molecular weight of 35.2 kDa. Tested by paper chromatography analysis using the complete hydrolysis by sulfuric acid, we found that the EP-1 comprise single component as glucose. The chemical structure of EP-1 was confirmed as a kind of linear glucan linked by β-(1,3) linkage. The Congo red reaction performed that EP-1 probable presented a triple-helical conformation in the dilute alkali
polysaccharide, structure analysis, Paecilomyces lilacinus
Structure type: homopolymer ; 35200
Location inside paper: Fig. 8
Trivial name: β-(1,3)-glucan
Compound class: glucan, polysaccharide
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: IR, inhibition studies, acid hydrolysis, Smith degradation, paper chromatography, UV, extraction, periodate oxidation, acetylation, reduction, column chromatography, complex formation with Congo Red, Sevag method
Related record ID(s): 3459, 42138, 42290, 42294, 43554, 47304, 47707, 48305, 48371, 48410, 48411, 48472, 49256, 49257, 49258, 49282, 49290, 102752
NCBI Taxonomy refs (TaxIDs): 33203Reference(s) to other database(s): GTC:G51056AN
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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(later renamed to: Purpureocillium lilacinum NH-PL-03)