Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Acinetobacter baumannii [ICD11:
XN8LS 
]
The structure was elucidated in this paperNCBI PubMed ID: 9063458Journal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Institutions: School of Chemistry, University of Hull, England, Institute fur Medizinishe Mikrobiologue und Hygiene, Universitat des Saarlandes, Homburg, Germany
A polysaccharide containing D-galactose (Gal), 2-acetamido-2-deoxy-D-galactose (GalNAc), 2-acetamido-2-deoxy-D-glucose (GlcNAc), and 3-deoxy-3-(D-3-hydroxybutyramido)-D-quinovose (Qui3NR) was isolated from lipopolysaccharide (LPS) obtained from cells walls of the reference strain for Acinetobacter baumannii O23. By means of NMR studies, methylation analysis, and chemical degradations, the repeating unit of the polymer was identified as a branched pentasaccharide with the structure 1. The same polymer was apparently also present in LPS of the reference strain for serogroup O12, together with a second polymer based on a branched tetrasaccharide with the structure 2. This second polymer has previously been isolated as the O16 antigen of A. baumannii [Haseley, S.R., Diggle, H.J. & Wilkinson, S. G. (1996) Carbohydr. Res. 293, 259-265] and is probably present as a minor component of the LPS of A. baumannii O11 [Haseley, S.R. & Wilkinson, S.G. (1996) Eur. J. Biochem. 237, 266-271]. [Sequence: see text]
Lipopolysaccharide, lipopolysaccharides, LPS, structure, strain, Acinetobacter, Acinetobacter baumannii, O antigens, reference
Structure type: polymer chemical repeating unit
Location inside paper: structure 1, abstract, p.150
Compound class: O-polysaccharide, O-antigen, CPS
Contained glycoepitopes: IEDB_130648,IEDB_135813,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_141807,IEDB_151528,IEDB_151531,IEDB_190606,SB_21,SB_7
Methods: NMR-2D, methylation, partial acid hydrolysis, NMR, Smith degradation, de-N-acylation/deamination
Biological activity: serological studies
Enzymes that release or process the structure: D-hydroxybutyrate dehydrogenase
Related record ID(s): 563, 564
NCBI Taxonomy refs (TaxIDs): 470
Show glycosyltransferases
NMR conditions: in D2O at 343 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,3,2 Ac
3,3 bDGlcpN 103.77 54.83 80.68 72.24 76.43 62.04
3,2 Ac
3,4,6,3 lR3HOBut
3,4,6 bDQuip3N 104.18 72.55 57.90 74.29 74.01 18.04
3,4,2 Ac
3,4 aDGlcpN 97.90 54.79 71.39 70.16 71.48 68.73
3 bDGalpN 104.11 52.65 79.53 74.47 76.07 61.46
aDGalp 100.13 68.45 80.04 69.91 71.22 60.85
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,3,2 Ac
3,3 bDGlcpN 4.58 3.69 3.76 3.62 3.45 3.72-3.92
3,2 Ac
3,4,6,3 lR3HOBut
3,4,6 bDQuip3N 4.55 3.40 3.86 3.23 3.56 1.33
3,4,2 Ac
3,4 aDGlcpN 4.91 3.93 3.88 3.72 4.36 4.09-4.15
3 bDGalpN 4.67 4.10 3.84 4.28 3.69 3.72
aDGalp 5.39 3.91 3.80 4.18 3.84 3.65
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,3,2 Ac
3,3 bDGlcpN 103.77/4.58 54.83/3.69 80.68/3.76 72.24/3.62 76.43/3.45 62.04/3.72-3.92
3,2 Ac
3,4,6,3 lR3HOBut
3,4,6 bDQuip3N 104.18/4.55 72.55/3.40 57.90/3.86 74.29/3.23 74.01/3.56 18.04/1.33
3,4,2 Ac
3,4 aDGlcpN 97.90/4.91 54.79/3.93 71.39/3.88 70.16/3.72 71.48/4.36 68.73/4.09-4.15
3 bDGalpN 104.11/4.67 52.65/4.10 79.53/3.84 74.47/4.28 76.07/3.69 61.46/3.72
aDGalp 100.13/5.39 68.45/3.91 80.04/3.80 69.91/4.18 71.22/3.84 60.85/3.65
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,3,2 | Ac | |
| 3,3 | bDGlcpN | 4.58 | 3.69 | 3.76 | 3.62 | 3.45 | 3.72
3.92 |
| 3,2 | Ac | |
| 3,4,6,3 | lR3HOBut | |
| 3,4,6 | bDQuip3N | 4.55 | 3.40 | 3.86 | 3.23 | 3.56 | 1.33 |
| 3,4,2 | Ac | |
| 3,4 | aDGlcpN | 4.91 | 3.93 | 3.88 | 3.72 | 4.36 | 4.09
4.15 |
| 3 | bDGalpN | 4.67 | 4.10 | 3.84 | 4.28 | 3.69 | 3.72 |
| | aDGalp | 5.39 | 3.91 | 3.80 | 4.18 | 3.84 | 3.65 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,3,2 | Ac | |
| 3,3 | bDGlcpN | 103.77 | 54.83 | 80.68 | 72.24 | 76.43 | 62.04 |
| 3,2 | Ac | |
| 3,4,6,3 | lR3HOBut | |
| 3,4,6 | bDQuip3N | 104.18 | 72.55 | 57.90 | 74.29 | 74.01 | 18.04 |
| 3,4,2 | Ac | |
| 3,4 | aDGlcpN | 97.90 | 54.79 | 71.39 | 70.16 | 71.48 | 68.73 |
| 3 | bDGalpN | 104.11 | 52.65 | 79.53 | 74.47 | 76.07 | 61.46 |
| | aDGalp | 100.13 | 68.45 | 80.04 | 69.91 | 71.22 | 60.85 |
|
There is only one chemically distinct structure:
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Displayed record 1
