Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperNCBI PubMed ID: 10542071Journal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Institutions: Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Sweden, Department of Immunology, Microbiology, Pathology and Infectious Diseases, Division of Clinical Bacteriology, Karolinska Institute, Huddinge University Hospital, Sweden, Department of Chemistry and Physiological Sciences, Universidad Nacional Autonoma de Nicaragua, UNAN-Leon, Nicaragua
The structure of the O-antigen polysaccharide of the lipopolysaccharide from an enteroaggregative Escherichia coli (strain 105) has been elucidated, using primarily one-dimensional and two-dimensional NMR experiments. The sequence of residues was deduced with heteronuclear multiple-bond correlation and NOESY experiments. The structure of the repeating unit of the polysaccharide from the enteroaggregative E. coli is as follows:[sequence: see text] The structure of the O-antigen from enteroaggregative E. coli strain 105 was shown to be identical with that of E. coli O21 by sugar and methylation analyses as well as by 1H NMR and 13C NMR spectroscopy.
NMR, structure, strain, structural, polysaccharide, O-antigen, Escherichia, Escherichia coli, O-antigenic, O-antigenic polysaccharide, Hafnia alvei, elucidation, structural studies, Enteroaggregative
Structure type: suggested polymer biological repeating unit
Location inside paper: abstract
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_135813,IEDB_136044,IEDB_137340,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_141807,IEDB_142487,IEDB_142488,IEDB_146664,IEDB_151531,IEDB_190606,IEDB_221845,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_21,SB_6,SB_7,SB_88
Methods: NMR-2D, methylation, NMR
Comments, role: O-antigenic polysaccharide, is identical to that of E. coli O21
Related record ID(s): 10389, 11682, 20649, 23242, 26890, 27435, 28337, 30360, 108742, 115996
NCBI Taxonomy refs (TaxIDs): 2162911Reference(s) to other database(s): GTC:G92807QP, GlycomeDB:
3591
Show glycosyltransferases
NMR conditions: in D2O at 348 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,4,4 bDGalp 103.6 72.4 73.9 69.7 75.8 61.9
3,4 bDGalp 103.9 71.3 82.5 76.1 75.3 61.6
3,2,2 Ac
3,2 bDGlcpN 102.2 56.8 75.1 70.9 76.8 61.8
3 bDGlcp 103.0 79.9 76.5 79.5 75.3 60.9
2 Ac
bDGalpN 104.0 52.5 80.6 68.7 75.5 62.0
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,4,4 bDGalp 4.86 3.51 3.71 3.93 3.68 3.78
3,4 bDGalp 4.47 3.74 3.83 4.38 3.74 3.77-3.82
3,2,2 Ac
3,2 bDGlcpN 4.87 3.72 3.54 3.46 3.38 3.77-3.92
3 bDGlcp 4.61 3.60 3.68 3.67 3.54 3.81-3.93
2 Ac
bDGalpN 4.78 3.97 3.94 4.20 3.69 3.79
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,4,4 bDGalp 103.6/4.86 72.4/3.51 73.9/3.71 69.7/3.93 75.8/3.68 61.9/3.78
3,4 bDGalp 103.9/4.47 71.3/3.74 82.5/3.83 76.1/4.38 75.3/3.74 61.6/3.77-3.82
3,2,2 Ac
3,2 bDGlcpN 102.2/4.87 56.8/3.72 75.1/3.54 70.9/3.46 76.8/3.38 61.8/3.77-3.92
3 bDGlcp 103.0/4.61 79.9/3.60 76.5/3.68 79.5/3.67 75.3/3.54 60.9/3.81-3.93
2 Ac
bDGalpN 104.0/4.78 52.5/3.97 80.6/3.94 68.7/4.20 75.5/3.69 62.0/3.79
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,4,4 | bDGalp | 4.86 | 3.51 | 3.71 | 3.93 | 3.68 | 3.78 |
| 3,4 | bDGalp | 4.47 | 3.74 | 3.83 | 4.38 | 3.74 | 3.77
3.82 |
| 3,2,2 | Ac | |
| 3,2 | bDGlcpN | 4.87 | 3.72 | 3.54 | 3.46 | 3.38 | 3.77
3.92 |
| 3 | bDGlcp | 4.61 | 3.60 | 3.68 | 3.67 | 3.54 | 3.81
3.93 |
| 2 | Ac | |
| | bDGalpN | 4.78 | 3.97 | 3.94 | 4.20 | 3.69 | 3.79 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,4,4 | bDGalp | 103.6 | 72.4 | 73.9 | 69.7 | 75.8 | 61.9 |
| 3,4 | bDGalp | 103.9 | 71.3 | 82.5 | 76.1 | 75.3 | 61.6 |
| 3,2,2 | Ac | |
| 3,2 | bDGlcpN | 102.2 | 56.8 | 75.1 | 70.9 | 76.8 | 61.8 |
| 3 | bDGlcp | 103.0 | 79.9 | 76.5 | 79.5 | 75.3 | 60.9 |
| 2 | Ac | |
| | bDGalpN | 104.0 | 52.5 | 80.6 | 68.7 | 75.5 | 62.0 |
|
There is only one chemically distinct structure:
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Found 1 record.
Displayed record 1
