Fucose-containing oligosaccharides (FCOs) have important applications in the food, medicine, and cosmetics industries owing to their unique biological activities. The degradation of microbial fucose-containing exopolysaccharide (FcEPS) is a promising strategy for obtaining FCOs, and bacteriophage-borne glycanase is a useful tool for degrading FcEPS. Here, we aimed to obtain FCOs using bacteriophage-borne glycanase to depolymerize FcEPS from Enterobacter sakazakii. The FcEPS was mainly composed of l-fucose (42.72 %), d-galactose (20.59 %), and d-glucose (21.81 %). Based on the results of nuclear magnetic resonance and mass spectrometry, the obtained FCOs were disaccharide fragments with backbones of β-d-Glcp-(1→4)-β-l-Fucp and α-d-Galp-(1→3)-β-l-Fucp, respectively. So far, few studies of disaccharides prepared from FcEPS have been reported. This study demonstrated that the FcEPS of E. sakazakii was a reliable fucose-containing disaccharide source and that bacteriophage-borne glycanase was an effective degradation tool for obtaining FCOs fragments from FcEPS.
fucose, Enterobacter sakazakii, bacteriophage-borne glycanase, fucose-containing disaccharide, microbial exopolysaccharide
Publication DOI: 10.1016/j.carbpol.2020.117139Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: mousun@ouc.edu.cn
Institutions: College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, Shandong, PR China, School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003, Shandong, PR China
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, HPLC, bacteriophage degradation, ESI-CID-MS/MS, TEM, bacteriophage characterization
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