Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: mycelium
NCBI PubMed ID: 31069486Publication DOI: 10.1007/s00253-019-09834-8Journal NLM ID: 8406612Publisher: Springer
Correspondence: Zhang J <zhangjinxia
caas.cn>
Institutions: Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, China, Key Laboratory of Microbial Resources, Ministry of Agriculture and Rural Affairs, Beijing, China
Trehalose is a nonreducing disaccharide, and it plays an intracellular protective role in organisms under various stress conditions. In this study, the trehalose synthesis and its protective role in Pleurotus ostreatus were investigated. As a signal in metabolic regulation, reactive oxygen species (ROS) accumulated in the mycelia of P. ostreatus under heat stress (HS). Furthermore, mycelial growth was significantly inhibited, and the malondialdehyde (MDA) level significantly increased under HS. First, exogenous addition of H2O2 inhibited mycelial growth and elevated the MDA level, while N-acetyl cysteine (NAC) and vitamin C (VC) reduced the MDA level and recovered mycelial growth under HS by scavenging ROS. These results indicated that the mycelial radial growth defect under HS might be partly caused by ROS accumulation. Second, adding NAC and VC to the media resulted in rescued trehalose accumulation, which indicated that ROS has an effect on inducing trehalose synthesis. Third, the mycelial growth was recovered by addition of trehalose to the media after HS, and the MDA level was reduced. This effect was further verified by the overexpression of genes for trehalose-6-phosphate synthase (TPS) and neutral trehalase (NTH), which led to increased and reduced trehalose content, respectively. In addition, adding validamycin A (NTH inhibitor) to the media promoted trehalose accumulation and the recovered mycelial growth after HS. In conclusion, trehalose production was partly induced by ROS accumulation in the mycelia under HS, and the accumulated trehalose could promote the recovery of growth after HS, partly by reducing the MDA level in the mycelia.
Trehalose, ROS, growth inhibition rate, heat stress, MDA
Structure type: oligomer
C
12H
22O
11Location inside paper: abstract
Trivial name: trehalose, trehalose dihydrate
Compound class: glucan, disaccharide, oligosaccharide, trehalose
Contained glycoepitopes: IEDB_142488,IEDB_144998,IEDB_146664,IEDB_742521,IEDB_983931,SB_192
Methods: RT-PCR, cell growth, fluorescence microscopy, confocal scanning laser microscopy, cloning plasmids, enzymatic assay, gene expression, RNA extraction, spectrophotometry, centrifugation, reverse transcription
Biological activity: heat stress induced ROS accumulation, which further inhibited mycelial growth in P. ostreatus. In addition, ROS acted as a signal to promote trehalose synthesis in the mycelia and thus to protect the mycelia against heat stress
Related record ID(s): 43110, 43368, 45048, 45464, 48342, 49503, 50340, 50354, 50364, 50595, 131827, 139902
NCBI Taxonomy refs (TaxIDs): 5322Reference(s) to other database(s): GTC:G92130SN, GlycomeDB:
245
Show glycosyltransferases
There is only one chemically distinct structure:
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