Cope KR, Bascaules A, Irving TB, Venkateshwaran M, Maeda J, Garcia K, Rush TA, Ma C, Labbé J, Jawdy S, Steigerwald E, Setzke J, Fung E, Schnell KG, Wang Y, Schlief N, Bücking H, Strauss SH, Maillet F, Jargeat P, Bécard G, Puech-Pagés V, Ané JM The ectomycorrhizal fungus Laccaria bicolor produces lipochitooligosaccharides and uses the common symbiosis pathway to colonize Populus roots Plant Cell31(10) (2019)
2386-2410
NCBI PubMed ID:31416823 Publication DOI:10.1105/tpc.18.00676 Journal NLM ID:9208688 Publisher: Rockville, MD: American Society of Plant Physiologists Correspondence: Ané JM <jeanmichel.anewisc.edu> Institutions: Department of Bacteriology, University of Wisconsin, Madison, USA, Department of Agronomy, University of Wisconsin, Madison, USA, Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, Castanet-Tolosan, France, Department of Forest Ecosystems and Society, Oregon State University, Corvallis, USA, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, USA, Department of Biology and Microbiology, South Dakota State University, Brookings, USA, Laboratoire des Interactions Plantes-Microorganismes, Université de Toulouse, INRA, CNRS, Castanet-Tolosan, France, Laboratoire Evolution et Diversité Biologique, Université de Toulouse, UPS, CNRS, IRD, Toulouse, France
Mycorrhizal fungi form mutualistic associations with the roots of most land plants and provide them with mineral nutrients from the soil in exchange for fixed carbon derived from photosynthesis. The common symbiosis pathway (CSP) is a conserved molecular signaling pathway in all plants capable of associating with arbuscular mycorrhizal fungi. It is required not only for arbuscular mycorrhizal symbiosis but also for rhizobia-legume and actinorhizal symbioses. Given its role in such diverse symbiotic associations, we hypothesized that the CSP also plays a role in ectomycorrhizal associations. We showed that the ectomycorrhizal fungus Laccaria bicolor produces an array of lipochitooligosaccharides (LCOs) that can trigger both root hair branching in legumes and, most importantly, calcium spiking in the host plant Populus in a CASTOR/POLLUX-dependent manner. Nonsulfated LCOs enhanced lateral root development in Populus in a calcium/calmodulin-dependent protein kinase (CCaMK)-dependent manner, and sulfated LCOs enhanced the colonization of Populus by L. bicolor Compared with the wild-type Populus, the colonization of CASTOR/POLLUX and CCaMK RNA interference lines by L. bicolor was reduced. Our work demonstrates that similar to other root symbioses, L. bicolor uses the CSP for the full establishment of its mutualistic association with Populus.
Cope KR, Bascaules A, Irving TB, Venkateshwaran M, Maeda J, Garcia K, Rush TA, Ma C, Labbé J, Jawdy S, Steigerwald E, Setzke J, Fung E, Schnell KG, Wang Y, Schlief N, Bücking H, Strauss SH, Maillet F, Jargeat P, Bécard G, Puech-Pagés V, Ané JM The ectomycorrhizal fungus Laccaria bicolor produces lipochitooligosaccharides and uses the common symbiosis pathway to colonize Populus roots Plant Cell31(10) (2019)
2386-2410
/Variants 0/-+ a-L-Fucp-(1-6)-+
| |
17HOOle-(1-2)-b-D-GlcpNMe-(1-4)-b-D-GlcpNAc-(1-4)-b-D-GlcpNAc
/Variants 0/ is:
Cm-6)-
OR (exclusively)
Cm-4)-
OR (exclusively)
Cm-3)-
NCBI PubMed ID:31416823 Publication DOI:10.1105/tpc.18.00676 Journal NLM ID:9208688 Publisher: Rockville, MD: American Society of Plant Physiologists Correspondence: Ané JM <jeanmichel.anewisc.edu> Institutions: Department of Bacteriology, University of Wisconsin, Madison, USA, Department of Agronomy, University of Wisconsin, Madison, USA, Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, Castanet-Tolosan, France, Department of Forest Ecosystems and Society, Oregon State University, Corvallis, USA, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, USA, Department of Biology and Microbiology, South Dakota State University, Brookings, USA, Laboratoire des Interactions Plantes-Microorganismes, Université de Toulouse, INRA, CNRS, Castanet-Tolosan, France, Laboratoire Evolution et Diversité Biologique, Université de Toulouse, UPS, CNRS, IRD, Toulouse, France
Mycorrhizal fungi form mutualistic associations with the roots of most land plants and provide them with mineral nutrients from the soil in exchange for fixed carbon derived from photosynthesis. The common symbiosis pathway (CSP) is a conserved molecular signaling pathway in all plants capable of associating with arbuscular mycorrhizal fungi. It is required not only for arbuscular mycorrhizal symbiosis but also for rhizobia-legume and actinorhizal symbioses. Given its role in such diverse symbiotic associations, we hypothesized that the CSP also plays a role in ectomycorrhizal associations. We showed that the ectomycorrhizal fungus Laccaria bicolor produces an array of lipochitooligosaccharides (LCOs) that can trigger both root hair branching in legumes and, most importantly, calcium spiking in the host plant Populus in a CASTOR/POLLUX-dependent manner. Nonsulfated LCOs enhanced lateral root development in Populus in a calcium/calmodulin-dependent protein kinase (CCaMK)-dependent manner, and sulfated LCOs enhanced the colonization of Populus by L. bicolor Compared with the wild-type Populus, the colonization of CASTOR/POLLUX and CCaMK RNA interference lines by L. bicolor was reduced. Our work demonstrates that similar to other root symbioses, L. bicolor uses the CSP for the full establishment of its mutualistic association with Populus.
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