Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Associated disease: infection due to Aspergillus fumigatus [ICD11:
XN5Z7 
]
Publication DOI: 10.1007/s11172-019-2713-yJournal NLM ID: 100912060Publisher: New York: Consultants Bureau
Correspondence: Nifantiev NE <nen
ioc.ac.ru>
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia, M.V. Lomonosov Moscow State University, Moscow, Russia
Galactomannan (GM) is a specific polysaccharide antigen of opportunistic mold fungi of the genus Aspergillus. The detection of GM in patients' biological fl uids is a reliable criterion for the diagnosis of invasive pulmonary aspergillosis, a severe disease. In this report, we evaluated the analytical capabilities of the fl uorescence polarization immunoassay (FPIA) for detection of GM. Using FPIA, the high affinity of the monoclonal antibody 7B8 to the synthetic oligosaccharide β-D-Galf-[(1→5)-β-D-Galf]3-(1→6)-α-D-Manp related to the galactomannan fragment was shown. The binding constant in this interaction was found to be equal to (1.02±0.01)•10^8 L/mol. The detection limit determined for this oligosaccharide with the competitive FPIA method is 3 ng/mL. The data obtained indicate the possibility of using FPIA for the diagnosis of invasive aspergillosis.
antigen, antibody, fluorescence, Galactomannan, Aspergillus fumigatus, diagnosticum
Structure type: oligomer
Location inside paper: abstract
Compound class: O-antigen, oligosaccharide
Contained glycoepitopes: IEDB_130701,IEDB_136095,IEDB_137472,IEDB_144983,IEDB_147453,IEDB_149137,IEDB_152206,IEDB_190606,IEDB_885812,IEDB_983930,SB_44,SB_67,SB_72
Methods: chemical synthesis, fluorescence polarization immunoassay
Synthetic data: chemical
NCBI Taxonomy refs (TaxIDs): 746128
Show glycosyltransferases
There is only one chemically distinct structure:
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