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Coelho JH, Eisele APP, Valezi CF, Mattos GJ, Schirmann JG, Dekker RFH, Barbosa-Dekker AM, Sartori ER
Exploring the exocellular fungal biopolymer botryosphaeran for laccase-biosensor architecture and application to determine dopamine and spironolactone
Talanta 204 (2019)
475–483
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?%b-D-Glcp-(1-6)-22%b-D-Glcp-(1-6)-+
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-3)-b-D-Glcp-(1-3)-b-D-Glcp-(1- |
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Botryosphaeria rhodina MAMB-05
(later renamed to: Lasiodiplodia theobromae MAMB-05)
(Ancestor NCBI TaxID 45133,
species name lookup)
Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
NCBI PubMed ID: 31357322Publication DOI: 10.1016/j.talanta.2019.06.033Journal NLM ID: 2984816RPublisher: Amsterdam: Elsevier
Correspondence: elensartori
uel.br
Institutions: Universidade Estadual de Londrina (UEL), Centro de Ciências Exatas, Departamento de Química, Londrina, Brazil, Universidade Tecnológica Federal do Paraná (UTFPR), Programa de Pós-Graduação em Engenharia Ambiental, Londrina, Brazil
Laccase was immobilized on a glassy carbon electrode layered with multi-walled carbon nanotubes using a film of botryosphaeran, a fungal (1→3)(1→6)-β-D-glucan. This novel biosensing platform was characterized by electrochemical impedance spectroscopy and scanning electron microscopy, and applied for the determination of dopamine. Experimental variables such as enzyme concentration, pH value and operational parameters of the electroanalytical technique were optimized. Using square-wave voltammetry, there was a linear dependence of peak current and dopamine concentration within the range of 2.99-38.5 μmol/L with a limit of detection of 0.127 μmol/L. The biosensor was successfully applied in the determination of dopamine in pharmaceutical injection and synthetic biological samples, and presented good selectivity even in the presence of uric acid and ascorbic acid, as well as other phenolic compounds. The different aspects regarding the operational stability of the laccase biosensor were evaluated, demonstrating good intra-day and inter-day repeatability, and long-storage stability. Furthermore, this biosensor was evaluated in the indirect determination of spironolactone by using the analytical signal of dopamine, presenting a limit of detection of 0.94 μmol/L. The results obtained in the analysis of spironolactone in commercial pharmaceutical samples were satisfactory.
Botryosphaeria rhodina MAMB-05, (1 → 3)(1 → 6)-β-D-glucan, dopamine, electrochemical biosensors, indirect determination, spironolactone
Structure type: structural motif or average structure
Location inside paper: p. 475, left column, paragraph 2
Trivial name: botryosphaeran
Compound class: EPS, glucan, polysaccharide
Contained glycoepitopes: IEDB_135614,IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_241101,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: cell growth, spectrophotometry, SEM, square-wave voltammetry, electrochemical impedance spectroscopy
Related record ID(s): 41787, 48304, 48397, 48442, 50037, 50070
NCBI Taxonomy refs (TaxIDs): 45133Reference(s) to other database(s): GTC:G59945WE
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Mattos GJ, Moraes JT, Barbosa ECM, Camargo PHC, Dekker RFH, Barbosa-Dekker AM, Sartori ER
Laccase stabilized on β-D-glucan films on the surface of carbon black/gold nanoparticles: A new platform for electrochemical biosensing
Bioelectrochemistry 129 (2019)
116-123
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?%b-D-Glcp-(1-6)-22%b-D-Glcp-(1-6)-+
|
-3)-b-D-Glcp-(1-3)-b-D-Glcp-(1- |
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Botryosphaeria rhodina MAMB-05
(later renamed to: Lasiodiplodia theobromae MAMB-05)
(Ancestor NCBI TaxID 45133,
species name lookup)
Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
NCBI PubMed ID: 31153126Publication DOI: 10.1016/j.bioelechem.2019.05.002Journal NLM ID: 100953583Publisher: Amsterdam: Elsevier
Correspondence: elensartori
uel.br
Institutions: Centro de Ciências Exatas, Departamento de Química, Universidade Estadual de Londrina, Londrina, Brazil, Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil, Programa de Pós-Graduação em Engenharia Ambiental, Universidade Tecnológica Federal do Paraná, Londrina, Brazil
In this study, (1→3)(1→6)-β-D-glucan (botryosphaeran) from Botryosphaeria rhodina MAMB-05 was used, for the first time, to immobilize laccase on a carbon black paste electrode modified with gold nanoparticles. The physicochemical characterization of the proposed laccase-biosensor was performed using transmission electron microscopy and electrochemical impedance spectroscopy. The performance of this novel bio-device was evaluated by choosing hydroquinone as a typical model of a phenolic compound. For hydroquinone determination, experimental variables such as enzyme concentration, pH and operational parameters of the electroanalytical technique were optimized. From square-wave voltammograms, a linear dependence between the cathodic current peak and the hydroquinone concentration was observed within the range 2.00-56.5 μmol/L, with a theoretical detection limit of 0.474 μmol/L. The proposed method was successfully applied to determine hydroquinone in dermatological cream, and samples from biological and environmental niches. The proposed biosensor device presented good selectivity in the presence of uric acid, various inorganic ions, as well as other phenolic compounds, demonstrating the potential application of this biosensing platform in complex matrices. Operational and analytical stability of the laccase biosensor were evaluated, and demonstrated good intra-day (SD=0.3%) and inter-day (SD=3.4%) repeatability and long storage stability (SD=4.9%).
Botryosphaeran, Botryosphaeria rhodina MAMB-05, phenolic compounds, (1→3)(1→6)-β-D-glucan, bioelectrochemistry, gold nanoparticles
Structure type: structural motif or average structure
Location inside paper: abstract
Trivial name: botryosphaeran
Compound class: EPS, glucan, polysaccharide
Contained glycoepitopes: IEDB_135614,IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_241101,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: UV, spectrophotometry, SEM, TEM, square-wave voltammetry, electrochemical impedance spectroscopy
Related record ID(s): 41787, 48304, 48397, 48442, 49980, 50070
NCBI Taxonomy refs (TaxIDs): 45133Reference(s) to other database(s): GTC:G59945WE
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Gomes A, Mattos GJ, Coldibeli B, Dekker RFH, Barbosa Dekker AM, Sartori ER
Covalent attachment of laccase to carboxymethyl-botryosphaeran in aqueous solution for the construction of a voltammetric biosensor to quantify quercetin
Bioelectrochemistry 135 (2020)
ID 107543
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?%b-D-Glcp-(1-6)-22%b-D-Glcp-(1-6)-+
|
-3)-b-D-Glcp-(1-3)-b-D-Glcp-(1- |
Show graphically |
Botryosphaeria rhodina MAMB-05
(later renamed to: Lasiodiplodia theobromae MAMB-05)
(Ancestor NCBI TaxID 45133,
species name lookup)
Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
NCBI PubMed ID: 32450282Publication DOI: 10.1016/j.bioelechem.2020.107543Journal NLM ID: 100953583Publisher: Amsterdam: Elsevier
Correspondence: elensartori
uel.br
Institutions: Universidade Tecnológica Federal do Paraná, Programa de Pós-Graduação em Engenharia Ambiental, Câmpus Londrina, Londrina, Brazil, Universidade Estadual de Londrina, Centro de Ciências Exatas, Departamento de Química, Londrina, Brazil
Laccase from Botryosphaeria rhodina MAMB-05 was covalently immobilized on carboxymethyl-botryosphaeran by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide (EDC/NHS) in aqueous solution. This approach was employed to fabricate a novel laccase-based biosensor to electrochemically quantify quercetin (QCT), using a simple carbon black paste electrode as a transducer. The proposed biosensor was characterized by electrochemical impedance spectroscopy and Nyquist plots were used to evaluate the immobilization of the enzyme. For determining QCT, variables were optimized, that included experimental conditions for laccase immobilization, pH of the supporting electrolyte, and instrumental parameters of the electroanalytical technique. From square-wave-voltammograms, a linear dependence between the cathodic current peak and QCT concentration was observed within the range 498-5000 μmol/L, with a theoretical detection limit of 260 μmol/L. The proposed method was successfully applied to determine QCT in beverages, pharmaceuticals, and biological samples. The proposed biosensor device presented good selectivity in the presence of uric acid, various inorganic ions, as well as other phenolic compounds, demonstrating the potential application of this biosensing platform in chemically complex solutions. Operational and analytical stability of the laccase-biosensor were evaluated, and good intra-day (SD = 1.23%) and inter-day (SD = 2.32%) repeatability, and long storage stability (SD = 3.47%) are presented.
β-glucan, biopolymer, flavonoids, electrochemical transduction, fungal laccase, voltammetry
Structure type: structural motif or average structure
Location inside paper: abstract
Trivial name: botryosphaeran
Compound class: EPS, glucan, polysaccharide
Contained glycoepitopes: IEDB_135614,IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_241101,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: HPLC, derivatization, square-wave voltammetry, electrochemical impedance spectroscopy
Related record ID(s): 41787, 48304, 48397, 48442, 50624, 50629, 50630, 50632
NCBI Taxonomy refs (TaxIDs): 45133Reference(s) to other database(s): GTC:G59945WE
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There is only one chemically distinct structure:
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Gomes A, Mattos GJ, Coldibeli B, Dekker RFH, Barbosa Dekker AM, Sartori ER
Covalent attachment of laccase to carboxymethyl-botryosphaeran in aqueous solution for the construction of a voltammetric biosensor to quantify quercetin
Bioelectrochemistry 135 (2020)
ID 107543
|
/Variants 0/-22%b-D-Glcp-(1-6)-+
|
-3)-b-D-Glcp-(1-3)-b-D-Glcp-(1-
/Variants 0/ is:
Gc-(1-6)-
OR (exclusively)
Gc-(1-6)-?%b-D-Glcp-(1-6)- |
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Botryosphaeria rhodina MAMB-05
(later renamed to: Lasiodiplodia theobromae MAMB-05)
(Ancestor NCBI TaxID 45133,
species name lookup)
Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
NCBI PubMed ID: 32450282Publication DOI: 10.1016/j.bioelechem.2020.107543Journal NLM ID: 100953583Publisher: Amsterdam: Elsevier
Correspondence: elensartori
uel.br
Institutions: Universidade Tecnológica Federal do Paraná, Programa de Pós-Graduação em Engenharia Ambiental, Câmpus Londrina, Londrina, Brazil, Universidade Estadual de Londrina, Centro de Ciências Exatas, Departamento de Química, Londrina, Brazil
Laccase from Botryosphaeria rhodina MAMB-05 was covalently immobilized on carboxymethyl-botryosphaeran by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide (EDC/NHS) in aqueous solution. This approach was employed to fabricate a novel laccase-based biosensor to electrochemically quantify quercetin (QCT), using a simple carbon black paste electrode as a transducer. The proposed biosensor was characterized by electrochemical impedance spectroscopy and Nyquist plots were used to evaluate the immobilization of the enzyme. For determining QCT, variables were optimized, that included experimental conditions for laccase immobilization, pH of the supporting electrolyte, and instrumental parameters of the electroanalytical technique. From square-wave-voltammograms, a linear dependence between the cathodic current peak and QCT concentration was observed within the range 498-5000 μmol/L, with a theoretical detection limit of 260 μmol/L. The proposed method was successfully applied to determine QCT in beverages, pharmaceuticals, and biological samples. The proposed biosensor device presented good selectivity in the presence of uric acid, various inorganic ions, as well as other phenolic compounds, demonstrating the potential application of this biosensing platform in chemically complex solutions. Operational and analytical stability of the laccase-biosensor were evaluated, and good intra-day (SD = 1.23%) and inter-day (SD = 2.32%) repeatability, and long storage stability (SD = 3.47%) are presented.
β-glucan, biopolymer, flavonoids, electrochemical transduction, fungal laccase, voltammetry
Structure type: structural motif or average structure
Location inside paper: Schematic 2, CMB
Compound class: polysaccharide
Contained glycoepitopes: IEDB_135614,IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_2278476,IEDB_2278477,IEDB_241101,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: HPLC, derivatization, square-wave voltammetry, electrochemical impedance spectroscopy
Related record ID(s): 50631
NCBI Taxonomy refs (TaxIDs): 45133
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There is only one chemically distinct structure:
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