Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: fruiting body
NCBI PubMed ID: 30876902Publication DOI: 10.1016/j.ijbiomac.2019.03.078Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: Song W <williamsong
doyepharma.com>; Guo S <SD-GUO
hotmail.com>
Institutions: Institute of Lipid metabolism and Atherosclerosis, Innovative Drug Research Centre, School of Pharmacy, Weifang Medical University, Weifang, China, Research Center on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin, China, Taishan Medical University, Taian, China
Water extracts of the edible mushroom Cordyceps militaris possess a lipid-lowering effect. However, the types of components and how they exert this effect are not clear. In this study, two novel polysaccharides, CM1 and CMS, were isolated, and their cholesterol efflux improving capacity was investigated in vitro. The molecular weight of CM1 was approximately 700 kDa, and its main chain was consisted of (1→4)-β-D-Glcp and (1→2)-α-D-manp branched at the O-6 positions of (1→2,6)-α-D-manp with (1→2) linked-β-D-galf, (1→2)-α-D-manp or methyl and terminated with β-D-Galf and α-D-Manp. The molecular weight of CMS was approximately 18.2 kDa, and it was a novel (1→6)-β-D-Glcp linked glucan. Both CM1 and CMS significantly increased [3H]-cholesterol efflux by activating the protein expression of ATP-binding cassette (ABC) G1. However, they showed no significant influence on the proteins expression of ABCA1 and scavenger receptor B type 1. Therefore, CM1 and CMS are effective water-soluble components with potential lipid-lowering activity. They may be exploited as potential candidates for dyslipidaemia-related diseases such as atherosclerosis.
NMR spectrometry, Cordyceps, lipid metabolism, cholesterol efflux, isotope tracing
Structure type: homopolymer ; 18200, n=98
Location inside paper: p. 269, Fig. 4, C
Trivial name: β-(1,6)-glucan
Compound class: glucan, polysaccharide
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, SDS-PAGE, acid hydrolysis, GC, Western blotting, biological assays, electrophoresis, extraction, acetylation, methylation analysis, reduction, dialysis, HPGPC, precipitation, phenol-sulfuric acid assay, derivatization, Bradford method, centrifugation
Biological activity: polysaccharide significantly improved cholesterol efflux in vitro by activating the protein expression of ABCG1
Comments, role: the terminal residue of this polysaccharide is aDGlcp
Related record ID(s): 50005
NCBI Taxonomy refs (TaxIDs): 73501Reference(s) to other database(s): GTC:G26777BZ
Show glycosyltransferases
1H NMR data: present in publication
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13C NMR data: present in publication
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There is only one chemically distinct structure:
Found 
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