Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Publication DOI: 10.1021/bk-2019-1309.ch004Publisher: American Chemical Society
Editors: Ilies MA, Sakurai K
Correspondence: sakurai
kitakyu-u.ac.jp
Institutions: Department of Chemistry, Aichi Institute of Technology, Aichi, Japan, Department of Chemistry and Biochemistry, University of Kitakyushu, Fukuoka, Japan
A β-1,3-D-glucan called schizophyllan (SPG) forms a novel complex with homo-polynucleotides such as poly(dA) via the combination of hydrogen bonding and hydrophobic interactions. The complex can protect the bound DNA from nuclease-mediated hydrolysis and nonspecific binding to serum proteins. Antigen-presenting cells (APCs), including macrophages and dendritic cells, are known to express β-glucan receptors. We have demonstrated that APCs recognize oligonucleotide (ODN)/SPG complexes, and the complexes are eventually ingested by the cells. This finding suggests that the bound ODN can exert its effect after internalization into cells. This chapter shows our recent in vitro and in vivo studies to prove this idea and provide a new strategy for specific delivery of functional ODNs including antisense (AS)-ODN, CpG-ODN, siRNA, and antigenic-peptide to APCs for treatment of diseases caused by disorders of the immune system.
immune system, schizophyllan, specific delivery
Structure type: polymer chemical repeating unit
Location inside paper: abstract, p. 80, Fig. 1
Trivial name: β-D-glucan, β-glucan, schizophyllan, carboxymethylglucan, pleuran, scleroglucan, lentinan, GRN, SPG, sizofiran, shizophyllan, Scleroglucan, lentinan-type beta-glucans (Ths-2), b-(1-3,6)-glucan, HEP3, grifolan LE, schizophyllan, scleroglucan, lentinan, termitan, grifolan, schizophyllan, scleroglucan, schizophyllan, sizofiran, grifolan, scleroglucan, schizophyllan, sonifilan, schizophyllan, grifolan, G. frondosa polysaccharide (GFP), alkali-soluble β-glucan (PeA3), schizophyllan (SPG), β-glucan, schizophyllan, β-glucan
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide, β-glucan, b-glucan, D-glucan, scleroglucan
Contained glycoepitopes: IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_241101,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: biological assays, flow cytometry analysis, cytokine production, cytotoxicity assay, derivatization, optical microscopy, quartz crystal microbalance
Related record ID(s): 40416, 40443, 41288, 41375, 41715, 41772, 41780, 41806, 41823, 41836, 41851, 42116, 42117, 42403, 42490, 43304, 43381, 43382, 43481, 43699, 43786, 43939, 43969, 44278, 44415, 44459, 44487, 44604, 45400, 45411, 45412, 45414, 45416, 45524, 45557, 45559, 45575, 45616, 45617, 45743, 45813, 45872, 45929, 47495, 47536, 48401, 48481, 48673, 48964, 49259, 49988, 50026, 50064, 50072, 50089, 100204, 106270, 110131, 111427, 111916, 112939, 113003, 115217, 115506, 116379, 118305, 125411, 139900, 140142, 141782, 144184, 296738
NCBI Taxonomy refs (TaxIDs): 5334Reference(s) to other database(s): GTC:G66305IS, CCSD:
49943, CBank-STR:12679, CA:9050-67-3
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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