School of Pharmaceutical Science, Jiangnan University, Wuxi, China, Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China, National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, China, Jiangsu Engineering Research Center for Bioactive Products Processing Technology, Jiangnan University, Wuxi, China, Jiangsu Shenhua Pharmaceutical Co. Ltd, Huaian, China
Polysaccharopeptide (PSP) from the medicinal mushroom Coriolus versicolor has been widely used in Asia as an adjunctive immunotherapy for treating cancers and liver diseases. However, the composition and structure of bioactive components in PSP remain elusive. Herein, we purified a hepatoprotective polysaccharide (PSP-1b1) with a molecular weight of 21.7 kDa from C. versicolor mycelia in submerged culture. PSP-1b1 consists of fucose, galactose, xylose, mannose, glucuronic acid and glucose at a relative molar ratio of 0.16:0.60:0.02:0.55:0.04:1.00. Structural features were investigated by methylation and gas chromatography-mass spectrometry, Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy. The PSP-1b1 backbone consists of →4)-α-Galp-(1→4)-α-Galp-(1→2)-α-Manp-(1→4)-α-Galp-(1→2)-α-Manp-(1→4)-α-Galp-(1→4)-α-Galp-(1→2)-α-Manp-(1→4)-α-Galp-(1→2)-α-Manp-(1→4)→, with branches of α-1,6-Manp, β-1,6-Glcp, β-1,3,6-Glcp, α-1,3-Manp, α-1,6-Galp, α-1,3-Fucp, T-α-Glcp and T-α-Galp on the O-6 position of α-Manp of the main chain, and secondary branches linked to the O-6 position of β-Glcp of the major branch. Treatment with PSK-1b1 (80 and 160 mg/kg/day) resulted in hepatoprotective effects against alcohol-induced liver injury in mice by reducing oxidative stress and modulating immunity.
p. 1108, Fig. 5, p. 1107, Table 1
13C NMR, 1H NMR, methylation, NMR-2D, IR, GC-MS, acid hydrolysis, Western blotting, biological assays, acetylation, methylation analysis, reduction, CC, HPGPC, precipitation, phenol-sulfuric acid assay, centrifugation, qRT-PCR, ion exchange chromatography, protein extraction
polysaccharide could effectively reduce the expression of TLR4, MyD88, CD14, IL-1β and TNF-α genes, and reduce the abundance of MyD88 and TNF-α proteins. This indicates that polysaccharide may reduce the expression of TNF-α and IL-1β by regulating the TLR4 signaling pathway, improve the immune balance of the body, and thus reduce alcohol-induced liver damage
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
4,4,2,4,2,4,4,2,4,2,4 aDGalp 97.94 69.61 69.99 78.43 71.27 60.68
4,4,2,4,2,4,4,2,4,2 aDGalp 97.94 69.61 69.99 78.43 71.27 60.68
4,4,2,4,2,4,4,2,4,6 bDGlcp 102.94 73.09 84.63 73.25 75.26 60.07
4,4,2,4,2,4,4,2,4 aDManp 101.43 78.46 70.37 69.91 72.14 69.18
4,4,2,4,2,4,4,2 aDGalp 97.94 69.61 69.99 78.43 71.27 60.68
4,4,2,4,2,4,4,6,6,2,3,6 aDGalp 102.08 68.78 71.15 69.98 70.11 60.68
4,4,2,4,2,4,4,6,6,2,3 aDManp 101.30 69.74 70.21 67.18 73.19 69.18
4,4,2,4,2,4,4,6,6,2,6,6,6,6 aDGalp 102.08 68.78 71.15 69.98 70.11 60.68
4,4,2,4,2,4,4,6,6,2,6,6,6 aDManp 101.30 69.74 70.21 67.18 73.19 69.18
4,4,2,4,2,4,4,6,6,2,6,6 bDGlcp 102.94 73.37 75.51 73.11 74.75 69.80
4,4,2,4,2,4,4,6,6,2,6 bDGlcp 102.94 73.37 75.51 73.11 74.75 69.80
4,4,2,4,2,4,4,6,6,2 bDGlcp 102.94 72.65 84.63 69.32 74.75 69.80
4,4,2,4,2,4,4,6,6 bDGalp 97.94 84.61 69.18 73.15 69.18 60.87
4,4,2,4,2,4,4,6 bDGlcp 102.94 73.37 75.51 73.11 74.75 69.80
4,4,2,4,2,4,4 aDManp 101.43 78.46 70.37 69.91 72.14 69.18
4,4,2,4,2,4 aDGalp 97.94 69.61 69.99 78.43 71.27 60.68
4,4,2,4,2 aDGalp 97.94 69.61 69.99 78.43 71.27 60.68
4,4,2,4,6,3,2,3 aDFucp 100.54 70.28 78.43 68.23 68.88 15.67
4,4,2,4,6,3,2 aDFucp 100.54 69.71 77.50 69.51 68.88 15.67
4,4,2,4,6,3 bDGalp 97.94 84.61 69.18 73.15 69.18 60.87
4,4,2,4,6 bDGlcp 102.94 73.09 84.63 73.25 75.26 60.07
4,4,2,4 aDManp 101.43 78.46 70.37 69.91 72.14 69.18
4,4,2 aDGalp 97.94 69.61 69.99 78.43 71.27 60.68
4,4,6,6,2,3,6 aDGalp 102.08 68.78 71.15 69.98 70.11 60.68
4,4,6,6,2,3 aDManp 101.30 69.74 70.21 67.18 73.19 69.18
4,4,6,6,2,6 bDGlcp 102.94 73.09 84.63 73.25 75.26 60.07
4,4,6,6,2 bDGlcp 102.94 72.65 84.63 69.32 74.75 69.80
4,4,6,6 bDGalp 97.94 84.61 69.18 73.15 69.18 60.87
4,4,6 bDGlcp 102.94 73.37 75.51 73.11 74.75 69.80
4,4 aDManp 101.43 78.46 70.37 69.91 72.14 69.18
4 aDGalp 97.94 69.61 69.99 78.43 71.27 60.68
aDGalp 97.94 69.61 69.99 78.43 71.27 60.68
4,4,2,4,2,4,4,2,4,6,3 aDGalp 102.08 68.78 71.15 69.98 70.11 60.68
4,4,2,4,2,4,4,2,4,6,3 aDGlcp 99.80 74.39 71.29 73.05 72.35 61.04
4,4,2,4,6,3,2,3,3 aDGalp 102.08 68.78 71.15 69.98 70.11 60.68
4,4,2,4,6,3,2,3,3 aDGlcp 99.80 74.39 71.29 73.05 72.35 61.04
4,4,6,6,2,6,3 aDGalp 102.08 68.78 71.15 69.98 70.11 60.68
4,4,6,6,2,6,3 aDGlcp 99.80 74.39 71.29 73.05 72.35 61.04
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
4,4,2,4,2,4,4,2,4,2,4 aDGalp 4.94 3.77 3.99 4.02 3.91 3.81-3.82
4,4,2,4,2,4,4,2,4,2 aDGalp 4.94 3.77 3.99 4.02 3.91 3.81-3.82
4,4,2,4,2,4,4,2,4,6 bDGlcp 4.65 3.31 3.56 3.42 3.24 3.31
4,4,2,4,2,4,4,2,4 aDManp 5.03 3.99 3.82 3.66 3.57 3.77-3.80
4,4,2,4,2,4,4,2 aDGalp 4.94 3.77 3.99 4.02 3.91 3.81-3.82
4,4,2,4,2,4,4,6,6,2,3,6 aDGalp 5.04 3.83 3.90 3.94 3.99 3.76-3.78
4,4,2,4,2,4,4,6,6,2,3 aDManp 4.97 3.98 3.76 3.68 3.57 3.77-3.80
4,4,2,4,2,4,4,6,6,2,6,6,6,6 aDGalp 5.04 3.83 3.90 3.94 3.99 3.76-3.78
4,4,2,4,2,4,4,6,6,2,6,6,6 aDManp 4.97 3.98 3.76 3.68 3.57 3.77-3.80
4,4,2,4,2,4,4,6,6,2,6,6 bDGlcp 4.43 3.24 3.41 3.66 3.82 3.94-3.99
4,4,2,4,2,4,4,6,6,2,6 bDGlcp 4.43 3.24 3.41 3.66 3.82 3.94-3.99
4,4,2,4,2,4,4,6,6,2 bDGlcp 4.45 3.43 3.66 3.41 3.82 3.94-3.99
4,4,2,4,2,4,4,6,6 bDGalp 4.90 3.44 3.77 3.57 3.81 3.67
4,4,2,4,2,4,4,6 bDGlcp 4.43 3.24 3.41 3.66 3.82 3.94-3.99
4,4,2,4,2,4,4 aDManp 5.03 3.99 3.82 3.66 3.57 3.77-3.80
4,4,2,4,2,4 aDGalp 4.94 3.77 3.99 4.02 3.91 3.81-3.82
4,4,2,4,2 aDGalp 4.94 3.77 3.99 4.02 3.91 3.81-3.82
4,4,2,4,6,3,2,3 aDFucp 5.21 3.99 4.02 4.08 4.13 1.14
4,4,2,4,6,3,2 aDFucp 5.27 4.05 3.99 3.82 4.08 1.14
4,4,2,4,6,3 bDGalp 4.90 3.44 3.77 3.57 3.81 3.67
4,4,2,4,6 bDGlcp 4.65 3.31 3.56 3.42 3.24 3.31
4,4,2,4 aDManp 5.03 3.99 3.82 3.66 3.57 3.77-3.80
4,4,2 aDGalp 4.94 3.77 3.99 4.02 3.91 3.81-3.82
4,4,6,6,2,3,6 aDGalp 5.04 3.83 3.90 3.94 3.99 3.76-3.78
4,4,6,6,2,3 aDManp 4.97 3.98 3.76 3.68 3.57 3.77-3.80
4,4,6,6,2,6 bDGlcp 4.65 3.31 3.56 3.42 3.24 3.31
4,4,6,6,2 bDGlcp 4.45 3.43 3.66 3.41 3.82 3.94-3.99
4,4,6,6 bDGalp 4.90 3.44 3.77 3.57 3.81 3.67
4,4,6 bDGlcp 4.43 3.24 3.41 3.66 3.82 3.94-3.99
4,4 aDManp 5.03 3.99 3.82 3.66 3.57 3.77-3.80
4 aDGalp 4.94 3.77 3.99 4.02 3.91 3.81-3.82
aDGalp 4.94 3.77 3.99 4.02 3.91 3.81-3.82
4,4,2,4,2,4,4,2,4,6,3 aDGalp 5.04 3.83 3.90 3.94 3.99 3.76-3.78
4,4,2,4,2,4,4,2,4,6,3 aDGlcp 5.31 3.55 3.77 3.46 3.84 3.66
4,4,2,4,6,3,2,3,3 aDGalp 5.04 3.83 3.90 3.94 3.99 3.76-3.78
4,4,2,4,6,3,2,3,3 aDGlcp 5.31 3.55 3.77 3.46 3.84 3.66
4,4,6,6,2,6,3 aDGalp 5.04 3.83 3.90 3.94 3.99 3.76-3.78
4,4,6,6,2,6,3 aDGlcp 5.31 3.55 3.77 3.46 3.84 3.66
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
4,4,2,4,2,4,4,2,4,2,4 aDGalp 97.94/4.94 69.61/3.77 69.99/3.99 78.43/4.02 71.27/3.91 60.68/3.81-3.82
4,4,2,4,2,4,4,2,4,2 aDGalp 97.94/4.94 69.61/3.77 69.99/3.99 78.43/4.02 71.27/3.91 60.68/3.81-3.82
4,4,2,4,2,4,4,2,4,6 bDGlcp 102.94/4.65 73.09/3.31 84.63/3.56 73.25/3.42 75.26/3.24 60.07/3.31
4,4,2,4,2,4,4,2,4 aDManp 101.43/5.03 78.46/3.99 70.37/3.82 69.91/3.66 72.14/3.57 69.18/3.77-3.80
4,4,2,4,2,4,4,2 aDGalp 97.94/4.94 69.61/3.77 69.99/3.99 78.43/4.02 71.27/3.91 60.68/3.81-3.82
4,4,2,4,2,4,4,6,6,2,3,6 aDGalp 102.08/5.04 68.78/3.83 71.15/3.90 69.98/3.94 70.11/3.99 60.68/3.76-3.78
4,4,2,4,2,4,4,6,6,2,3 aDManp 101.30/4.97 69.74/3.98 70.21/3.76 67.18/3.68 73.19/3.57 69.18/3.77-3.80
4,4,2,4,2,4,4,6,6,2,6,6,6,6 aDGalp 102.08/5.04 68.78/3.83 71.15/3.90 69.98/3.94 70.11/3.99 60.68/3.76-3.78
4,4,2,4,2,4,4,6,6,2,6,6,6 aDManp 101.30/4.97 69.74/3.98 70.21/3.76 67.18/3.68 73.19/3.57 69.18/3.77-3.80
4,4,2,4,2,4,4,6,6,2,6,6 bDGlcp 102.94/4.43 73.37/3.24 75.51/3.41 73.11/3.66 74.75/3.82 69.80/3.94-3.99
4,4,2,4,2,4,4,6,6,2,6 bDGlcp 102.94/4.43 73.37/3.24 75.51/3.41 73.11/3.66 74.75/3.82 69.80/3.94-3.99
4,4,2,4,2,4,4,6,6,2 bDGlcp 102.94/4.45 72.65/3.43 84.63/3.66 69.32/3.41 74.75/3.82 69.80/3.94-3.99
4,4,2,4,2,4,4,6,6 bDGalp 97.94/4.90 84.61/3.44 69.18/3.77 73.15/3.57 69.18/3.81 60.87/3.67
4,4,2,4,2,4,4,6 bDGlcp 102.94/4.43 73.37/3.24 75.51/3.41 73.11/3.66 74.75/3.82 69.80/3.94-3.99
4,4,2,4,2,4,4 aDManp 101.43/5.03 78.46/3.99 70.37/3.82 69.91/3.66 72.14/3.57 69.18/3.77-3.80
4,4,2,4,2,4 aDGalp 97.94/4.94 69.61/3.77 69.99/3.99 78.43/4.02 71.27/3.91 60.68/3.81-3.82
4,4,2,4,2 aDGalp 97.94/4.94 69.61/3.77 69.99/3.99 78.43/4.02 71.27/3.91 60.68/3.81-3.82
4,4,2,4,6,3,2,3 aDFucp 100.54/5.21 70.28/3.99 78.43/4.02 68.23/4.08 68.88/4.13 15.67/1.14
4,4,2,4,6,3,2 aDFucp 100.54/5.27 69.71/4.05 77.50/3.99 69.51/3.82 68.88/4.08 15.67/1.14
4,4,2,4,6,3 bDGalp 97.94/4.90 84.61/3.44 69.18/3.77 73.15/3.57 69.18/3.81 60.87/3.67
4,4,2,4,6 bDGlcp 102.94/4.65 73.09/3.31 84.63/3.56 73.25/3.42 75.26/3.24 60.07/3.31
4,4,2,4 aDManp 101.43/5.03 78.46/3.99 70.37/3.82 69.91/3.66 72.14/3.57 69.18/3.77-3.80
4,4,2 aDGalp 97.94/4.94 69.61/3.77 69.99/3.99 78.43/4.02 71.27/3.91 60.68/3.81-3.82
4,4,6,6,2,3,6 aDGalp 102.08/5.04 68.78/3.83 71.15/3.90 69.98/3.94 70.11/3.99 60.68/3.76-3.78
4,4,6,6,2,3 aDManp 101.30/4.97 69.74/3.98 70.21/3.76 67.18/3.68 73.19/3.57 69.18/3.77-3.80
4,4,6,6,2,6 bDGlcp 102.94/4.65 73.09/3.31 84.63/3.56 73.25/3.42 75.26/3.24 60.07/3.31
4,4,6,6,2 bDGlcp 102.94/4.45 72.65/3.43 84.63/3.66 69.32/3.41 74.75/3.82 69.80/3.94-3.99
4,4,6,6 bDGalp 97.94/4.90 84.61/3.44 69.18/3.77 73.15/3.57 69.18/3.81 60.87/3.67
4,4,6 bDGlcp 102.94/4.43 73.37/3.24 75.51/3.41 73.11/3.66 74.75/3.82 69.80/3.94-3.99
4,4 aDManp 101.43/5.03 78.46/3.99 70.37/3.82 69.91/3.66 72.14/3.57 69.18/3.77-3.80
4 aDGalp 97.94/4.94 69.61/3.77 69.99/3.99 78.43/4.02 71.27/3.91 60.68/3.81-3.82
aDGalp 97.94/4.94 69.61/3.77 69.99/3.99 78.43/4.02 71.27/3.91 60.68/3.81-3.82
4,4,2,4,2,4,4,2,4,6,3 aDGalp 102.08/5.04 68.78/3.83 71.15/3.90 69.98/3.94 70.11/3.99 60.68/3.76-3.78
4,4,2,4,2,4,4,2,4,6,3 aDGlcp 99.80/5.31 74.39/3.55 71.29/3.77 73.05/3.46 72.35/3.84 61.04/3.66
4,4,2,4,6,3,2,3,3 aDGalp 102.08/5.04 68.78/3.83 71.15/3.90 69.98/3.94 70.11/3.99 60.68/3.76-3.78
4,4,2,4,6,3,2,3,3 aDGlcp 99.80/5.31 74.39/3.55 71.29/3.77 73.05/3.46 72.35/3.84 61.04/3.66
4,4,6,6,2,6,3 aDGalp 102.08/5.04 68.78/3.83 71.15/3.90 69.98/3.94 70.11/3.99 60.68/3.76-3.78
4,4,6,6,2,6,3 aDGlcp 99.80/5.31 74.39/3.55 71.29/3.77 73.05/3.46 72.35/3.84 61.04/3.66
report error
Found 1 record.
Displayed record 1
(later renamed to: Trametes versicolor)
jiangnan.edu.cn>; Xu ZH <zhenghxu