Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wallAssociated disease: infection due to Aspergillus fumigatus [ICD11:
XN5Z7 
];
infection due to Aspergillus flavus [ICD11:
XN6B8 ]
Publication DOI: 10.1007/s12649-019-00746-2Journal NLM ID: 101670525Publisher: Dordrecht, Netherlands: Springer
Correspondence: saqader
uok.edu.pk
Institutions: The Karachi Institute of Biotechnology & Genetic Engineering (KIBGE), University of Karachi, Karachi, Pakistan, Department of Biochemistry, University of Karachi, Karachi, Pakistan
Chitin is a unique structural exopolysaccharide abundantly found in nature. This exopolysaccharide has a unique chemical structure that acts as a protective outermost covering for most of the crustaceans in aquatic ecosystem. This fortification is because of the insoluble nature of this exopolysaccharide which consist of a linear chain of β-(1→4)-linked-N-acetylglucosamine units. Chitin is hydrolyzed with the help of a hydrolase known as chitinase. Variety of microbial species have been explored for chitinase production. Chitinolytic microbial species can be alternatively used for degradation of chitin instead of chemical treatment in agricultural sector. This biological approach has lesser environmental impact because of its apparently safe nature. In the current study, bioprospecting of chitinase producing species was conducted and different chitinolytic bacterial strains were screened for chitinase production which could have anti-fungal potential. Bacterial isolates were identified based on polyphasic approach and the enzyme production was optimized using one-variable-at-a-time technique. Hyphal extension method was used for determination of anti-fungal potential of chitinase.Glutamicibacter uratoxydans was indigenously isolated and identified for chitinase production. G. uratoxydans is a novel bacterial species which has not been previously explored to produce chitinase or other hydrolases. G. uratoxydans biosynthesized chitinase utilizing colloidal chitin as a sole source of carbon. The chitinase biosynthesized by G. uratoxydans is effectively potent against Aspergillus fumigatus thus, suggesting that this extracellular enzyme could be used for the treatment of fungal infection caused by filamentous fungi.
cell wall, antifungal activity, chitin, chitinase, Glutamicibacter uratoxydans
Structure type: homopolymer
Location inside paper: abstract
Trivial name: chitin
Compound class: O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide, chitin
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_141807,IEDB_151531,IEDB_153212,IEDB_241099,IEDB_423114,IEDB_423150,SB_74,SB_85
Methods: PCR, cell growth, enzymatic assay, DNA extraction, precipitation, SEM, centrifugation, antifungal activity test, protein determination, BLAST
Enzymes that release or process the structure: chitinase
Related record ID(s): 40760, 40800, 41831, 49862, 50302, 50306, 50309, 50312, 50313, 50316, 50318, 111874, 121703, 131817, 139821, 143656, 147987, 149876
NCBI Taxonomy refs (TaxIDs): 746128,
5059,
33178,
5061Reference(s) to other database(s): GTC:G97099AY, CCSD:
46067, CBank-STR:5851, GenDB:KF905651
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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