Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: basidiocarp
NCBI PubMed ID: 32856730Publication DOI: 10.1002/bab.2013Journal NLM ID: 8609465Correspondence: Kuppusamy UR <umah
um.edu.my>
Institutions: Mushroom Research Centre, University of Malaya, Kuala Lumpur, Malaysia, Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia, Institute of Biological Science, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia
Ganoderma neo-japonicum is a well-known medicinal mushroom in Asian countries. However, scientific validations on its curative activities are confined to cirrhosis and diabetes. In this study, the anticancer properties of G. neo-japonicum were evaluated using cellular and computational models. The ethanolic extract (EtOH) with a promising inhibitory effect was fractionated into four different fractions: hexane (Hex), chloroform (Chl), butanol (Btn), and aqueous (Aq). The active fractions were then subjected to cell apoptosis assessment and phytochemical profiling. Molecular docking was conducted to elucidate the affinity of selected constituents towards antiapoptotic Bcl-2 protein. The butanol fraction showed the highest antioxidant activities as well as total phenolic content. Both hexane and chloroform fractions exerted a potent cytotoxic effect on colonic carcinoma cells through the induction of apoptosis. Phytochemical analysis revealed that the chloroform fraction is terpenoid enriched whereas the hexane fraction comprises predominantly sterol constituents. Stellasterol and 1,25-dihydroxyvitamin D3 3-glycoside were demonstrated to have a high affinity towards Bcl-2 protein. Overall, G. neo-japonicum can be considered as a compelling therapeutic candidate for cancer treatment.
apoptosis, Antioxidant, molecular docking, Anticancer, Ganoderma neo-japonicum
Structure type: monomer ; 530.60
C
30H
42O
8Location inside paper: Table 5, 3
Trivial name: proscillaridin A
Compound class: glycoside, terpenoid glycoside
Contained glycoepitopes: IEDB_136105,IEDB_225177,IEDB_885823
Methods: GC-MS, extraction, LC-MS, cell growth, antioxidant activities, cell viability assay, radical scavenging assay, evaporation, MTT, filtration, Folin phenol reagent method, binding assay, apoptosis assay
Biological activity: binding data
Related record ID(s): 50416, 50417, 50418
NCBI Taxonomy refs (TaxIDs): 36073
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error