Ganoderma neo-japonicum is a well-known medicinal mushroom in Asian countries. However, scientific validations on its curative activities are confined to cirrhosis and diabetes. In this study, the anticancer properties of G. neo-japonicum were evaluated using cellular and computational models. The ethanolic extract (EtOH) with a promising inhibitory effect was fractionated into four different fractions: hexane (Hex), chloroform (Chl), butanol (Btn), and aqueous (Aq). The active fractions were then subjected to cell apoptosis assessment and phytochemical profiling. Molecular docking was conducted to elucidate the affinity of selected constituents towards antiapoptotic Bcl-2 protein. The butanol fraction showed the highest antioxidant activities as well as total phenolic content. Both hexane and chloroform fractions exerted a potent cytotoxic effect on colonic carcinoma cells through the induction of apoptosis. Phytochemical analysis revealed that the chloroform fraction is terpenoid enriched whereas the hexane fraction comprises predominantly sterol constituents. Stellasterol and 1,25-dihydroxyvitamin D3 3-glycoside were demonstrated to have a high affinity towards Bcl-2 protein. Overall, G. neo-japonicum can be considered as a compelling therapeutic candidate for cancer treatment.

apoptosis, Antioxidant, molecular docking, Anticancer, Ganoderma neo-japonicum

There is only one chemically distinct structure:

SVGMWSMILES3D molIonize

 

C[C@@H]1O[C@@H](O[C@@H]2C=C3CC[C@@H]4[C@H](CC[C@]5(C)[C@@H](c6ccc(=O)oc6)CC[C@]45O)[C@@]3(C)CC2)[C@H](O)[C@H](O)[C@H]1O

530.658 g/mol (C₃₀H₄₂O₈)

A rearranged cadinane sesquiterpene glycoside named tinocordiside (1), consisting of a tricyclic skeleton with a cyclobutane ring, has been isolated from the immunomodulatory aqueous fraction of the Indian medicinal plant Tinospora cordifolia. The structure has been established by spectroscopic and chemical methods.

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5	C6
11	bDGlcp	97.0	71.5	77.5	74.5	76.5	62.5
	Subst


1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5	H6
11	bDGlcp	3.7	4.4	3.03	3.53	3.10	3.20
	Subst


1H/13C HSQC data:
Linkage	Residue	C1/H1	C2/H2	C3/H3	C4/H4	C5/H5	C6/H6
11	bDGlcp	97.0/3.7	71.5/4.4	77.5/3.03	74.5/3.53	76.5/3.10	62.5/3.20
	Subst

There is only one chemically distinct structure:

SVGMWSMILES3D molIonize

 

CC1=CC(=O)C2[C@@H]3[C@H](C(C)(C)O[C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)CC[C@]2(C)[C@H]13

396.48 g/mol (C₂₁H₃₂O₇)

A new monoterpene glycoside named paeonivayin with other seven known compounds were isolated from the roots of Paeonia delavayi Franch. and their structures were determined by means of spectroscopic studies.

monoterpene glycoside, Paeonia delavayi Franch, paeoniaceae, paeonivayin

There is only one chemically distinct structure:

SVGMWSMILES3D molIonize

 

C[C@]12C[C@@H](O)C3C[C@]1(O[C@@H]1O[C@H](COC(=O)c4ccccc4)[C@@H](O)[C@H](O)[C@H]1O)[C@]3(COC(=O)c1ccccc1)C(=O)O2

584.574 g/mol (C₃₀H₃₂O₁₂)