Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
NCBI PubMed ID: 33186018Publication DOI: 10.1021/acs.biomac.0c01038Journal NLM ID: 100892849Publisher: Washington, DC: American Chemical Society
Correspondence: sakurai
kitakyu-u.ac.jp
Institutions: Department of Chemistry and Biochemistry, University of Kitakyushu, Kitakyushu, Japan
Oligo-deoxyadenylic acid (dAX) forms a novel 1:2 triple-helix with β-1,3-D-glucan schizophyllan (SPG). We found that dAX meticulously selects the most suitable length of SPG to bind; for example, dA30 only complexes with a short SPG chain having 30, 60, or 90 main-chain glucoses, and they can be easily isolated with each other. This study demonstrated such a novel stoichiometric complex formation by using gel permeation chromatography coupled with multi-angle light scattering and synchrotron small-angle X-ray scattering. These oligo-DNA/polysaccharide complexes can be used as a tool for delivering therapeutic oligonucleotides to immunocytes that express the β-1,3-D-glucan receptors. The present study provides a robust platform technique to characterize them in terms of modern regulatory science of nanomedicines, which is requisite to transfer drug candidates into clinical trial. Our findings are important for characterizing these complexes as well as for providing a new insight into nucleotide and saccharide chemistry.
binding, schizophyllan, triple-helix, oligo-DNA, delivering, nanomedicine
Structure type: polymer chemical repeating unit
Location inside paper: Fig. 1, A
Trivial name: β-D-glucan, β-glucan, schizophyllan, carboxymethylglucan, pleuran, scleroglucan, lentinan, GRN, SPG, sizofiran, shizophyllan, Scleroglucan, lentinan-type beta-glucans (Ths-2), b-(1-3,6)-glucan, HEP3, grifolan LE, schizophyllan, scleroglucan, lentinan, termitan, grifolan, schizophyllan, scleroglucan, schizophyllan, sizofiran, grifolan, scleroglucan, schizophyllan, sonifilan, schizophyllan, grifolan, G. frondosa polysaccharide (GFP), alkali-soluble β-glucan (PeA3), schizophyllan (SPG), β-glucan, schizophyllan, β-glucan
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide, β-glucan, b-glucan, D-glucan, scleroglucan
Contained glycoepitopes: IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_241101,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, viscosity measurement, CD, elemental analysis, dialysis, precipitation, sonication, filtration, GPC-MALLS, SAXS, qNMR
Related record ID(s): 43304, 43969, 45411, 45414, 45813, 50613, 50621, 50636, 50640, 50642, 50645, 50646, 50652, 50656, 50662, 50663, 50664, 50676, 106270, 113003, 115217
NCBI Taxonomy refs (TaxIDs): 5334Reference(s) to other database(s): GTC:G66305IS, CCSD:
49943, CBank-STR:12679, CA:9050-67-3
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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