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Taxonomic group: fungi / Basidiomycota (Phylum: Basidiomycota)
Organ / tissue: fruiting body

The structure was elucidated in this paper
NCBI PubMed ID: 32437807
Publication DOI: 10.1016/j.ijbiomac.2020.05.054
Journal NLM ID: 7909578
Publisher: Butterworth-Heinemann
Correspondence: Ji H-Y <17908007

mail.tust.edu.cn>; Ji H-Y <haiyu11456

mail.tust.edu.cn>; Liu A-J <laj

tust.edu.cn>
Institutions: State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, China, QingYunTang Biotech (Beijing) Co., Ltd., Beijing, China

The edible mushroom G. frondosa has been used as a kind of functional food for the prevention and therapy of various diseases in Asian countries. In the present work, a novel acid-soluble polysaccharide (GFAP) was successfully isolated from G. frondosa under room temperature and hydrochloric acid solution treatment. Results of chemical composition analysis, UV and HPGPC spectra showed that GFAP mainly contained 94.28% of carbohydrate with the average molecular weight of about 644.9 kDa. GC, FT-IR, NMR and methylation analysis further indicated that GFAP was a neutral sugar mainly composed of (1→3)-β-D-Glcp and (1→3)-α-D-Manp. The in vivo antitumor experiments demonstrated that GFAP could effectively protect thymuses and spleens of tumor-bearing mice and inhibit the growth of H22 solid tumors with the inhibitory rate of 36.72%. Besides, GFAP could significantly improve the activities of NK cells, macrophages, CD19+ B cells and CD4+ T cells, leading to the apoptosis of H22 cells via G0/G1 phase arrested. Our data demonstrated that GFAP holds great application prospect to be a safe and effective antitumor adjuvant in the future.

anti-tumor activity, structural characteristics, Grifola frondosa acid-soluble polysaccharide

Structure type: structural motif or average structure ; 644900
Location inside paper: p. 1293, Fig. 4
Trivial name: glucomannan
Compound class: polysaccharide
Contained glycoepitopes: IEDB_115576,IEDB_130701,IEDB_1397514,IEDB_140116,IEDB_140628,IEDB_141793,IEDB_141806,IEDB_142488,IEDB_144983,IEDB_146664,IEDB_152206,IEDB_153220,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_164174,IEDB_241101,IEDB_558867,IEDB_558869,IEDB_857743,IEDB_983930,IEDB_983931,SB_192,SB_197,SB_198,SB_44,SB_67,SB_72,SB_73

Methods: 13C NMR, 1H NMR, NMR-2D, methylation, IR, acid hydrolysis, GC, biological assays, UV, extraction, acetylation, methylation analysis, reduction, column chromatography, dialysis, HPGPC, precipitation, phenol-sulfuric acid assay, carbazole-sulfuric acid assay, antitumor activity assay, sonication, Sevag method, Coomassie brilliant blue method
Biological activity: polysaccharide enhanced the killing activity of NK cells, pinocytosis ability of macrophages, and the activities of B cells and CD4+ T cells, finally leading to the apoptosis H22 solid tumors cells in vivo via G0/G1 phase arrested

Related record ID(s): 50921
NCBI Taxonomy refs (TaxIDs): 5627
Reference(s) to other database(s): GTC:G67683GP
Show glycosyltransferases

NMR conditions: in D2O at 298 K

¹H NMR data: present in publication

¹³C NMR data: present in publication

There are 3 chemically distinct structures. Please, select:

[*]O[C@H]1[C@H](O)[C@@H](CO)O[C@@H](O[C@@H]2[C@@H](O)[C@H](O[C@@H]3[C@@H](O)[C@H](O[C@@H]4[C@@H](O)[C@H](O[C@@H]5[C@@H](O)[C@H](O[C@@H]6[C@@H](O)[C@H](O[C@@H]7[C@@H](O)[C@H](O[C@@H]8[C@@H](O)[C@H](O[C@@H]9[C@@H](O)[C@H]([*])O[C@H](CO)[C@H]9O)O[C@H](CO)[C@H]8O)O[C@H](CO)[C@H]7O)O[C@H](CO[C@H]7O[C@H](CO)[C@@H](O)[C@H](O[C@H]8O[C@H](CO[C@H]9O[C@H](CO)[C@@H](O)[C@H](O[C@@H]%10O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%10O)[C@@H]9O)[C@@H](O)[C@H](O[C@H]9O[C@H](CO[C@H]%10O[C@H](CO)[C@@H](O)[C@H](O[C@@H]%11O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%11O)[C@@H]%10O)[C@@H](O)[C@H](O[C@H]%10O[C@H](CO)[C@@H](O)[C@H](O[C@H]%11O[C@H](CO)[C@@H](O)[C@H](O[C@H]%12O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]%12O)[C@@H]%11O)[C@@H]%10O)[C@@H]9O)[C@@H]8O)[C@@H]7O)[C@H]6O)O[C@H](CO)[C@H]5O)O[C@H](CO)[C@H]4O)O[C@H](CO[C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)[C@H]3O)O[C@H](CO)[C@H]2O)[C@@H]1O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O[C@@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H]1O

SVG MW SMILES 3D mol Ionize