Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Associated disease: infection due to Streptococcus pneumoniae [ICD11:
XN3PW 
]
The structure was elucidated in this paperNCBI PubMed ID: 32688016Publication DOI: 10.1016/j.carres.2020.108024Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: C. Ma <cma
gsu.edu>; W. Huang <wei.huang6
pfizer.com>
Institutions: Department of Chemistry, Georgia State University, Atlanta, GA, 30303, United States, Analytical Research and Development, BioTherapeutics Pharmaceutical Sciences, Pfizer, Inc., Chesterfield, MO, USA, Georgia Highlands College, 5901 Stewart Pkwy, Douglasville, GA, 30135, United States
Although there have been decades of research on streptococcus pneumoniae, it is still among the leading cause of infectious disease in the world. As a type of capsular polysaccharide (CPS) of streptococcus pneumoniae, pneumococcal polysaccharides are essential components for colonization and virulence in mammalian hosts. This study aimed to characterize the CPS structure of type 8 streptococcus pneumoniae, which is one of the most fatal serotypes. In this work, heparinase I&III was used to successfully digest pneumococcal type 8 polysaccharide (Pn8P). We characterized the oligosaccharide generated from the enzymatic depolymerization of Pn8P by size exclusion chromatography, mass spectrometry and nuclear magnetic resonance. This is the first study to enzymatically depolymerize and characterize Pn8P.
NMR, mass spectrometry, vaccine, pneumococcal polysaccharide, Heparinase, Size exclusion chromatography
Structure type: oligomer
Location inside paper: Fig.4, the digested CPS
Compound class: CPS
Contained glycoepitopes: IEDB_136044,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_190606,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_7,SB_88
Methods: 13C NMR, 1H NMR, NMR-2D, ESI-MS, enzymatic degradation, SEC, HPLC-ELSD, HPLC-RI-MS
Enzymes that release or process the structure: heparinase
Comments, role: oligosaccharide was obtained by heparinase digestion of Pn8P.
Related record ID(s): 32155
NCBI Taxonomy refs (TaxIDs): 1313
Show glycosyltransferases
NMR conditions: in D2O at 333 K
1H NMR data: present in publication
|
13C NMR data: present in publication
|
There is only one chemically distinct structure:
Found 
report error