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Taxonomic group: fungi / Basidiomycota (Phylum: Basidiomycota)
Organ / tissue: fruiting bodies

The structure was elucidated in this paper
NCBI PubMed ID: 36618684
Publication DOI: 10.3389/fnut.2022.1058131
Journal NLM ID: 101642264
Publisher: Lausanne, Switzerland: Frontiers Media S.A.
Correspondence: L. He <kite006

126.com>; H. Wei <whlwlp

163.com>
Institutions: Key Laboratory of Biological and Chemical Utilization of Zhejiang Forest Resources, Department of Forest Foods, Zhejiang Academy of Forestry, Hangzhou, China, Institute of Horticulture, Hangzhou Academy of Agricultural Sciences, Hangzhou, China, Institute of Biochemistry, College of Life Sciences, Institute of Biochemistry, Zhejiang University, Hangzhou, China, Huzhou Liangxi Forest Park Management Office, Huzhou, China, School of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou, China, Center of Forecasting and Analysis, Zhejiang Chinese Medical University, Hangzhou, China

A biomacromolecule, named as β-galactoglucofurannan (SVPS2), was isolated from the cultivated parts of Sanghuangporus vaninii under the forest. Its primary and advanced structure was analyzed by a series of techniques including GC-MS, methylation, NMR, MALS as well as AFM. The results indicated that SVPS2 was a kind of 1, 5-linked β-Glucofurannan consisting of β-glucose, β-galactose and α-fucose with 23.4 KDa. It exhibited a single-stranded chain with an average height of 0.72 nm in saline solution. The immunostimulation test indicated SVPS2 could facilitate the initiation of the immune reaction and promote the secretion of cytokines in vitro. Moreover, SVPS2 could mediate the apoptosis of HT-29 cells by blocking them in S phase. Western blot assay revealed an upregulation of Bax, Cytochrome c and cleaved caspase-3 by SVPS2, accompanied by a downregulation of Bcl-2. These results collectively demonstrate that antitumor mechanism of SVPS2 may be associated with enhancing immune response and inducing apoptosis of tumor cells in vitro. Therefore, SVPS2 might be utilized as a promising therapeutic agent against colon cancer and functional food with immunomodulatory activity.

conformation, structure, antitumor activity, Sanghuangporus vaninii

Structure type: structural motif or average structure ; 7000
Location inside paper: table 2, Fig. 3, SVPS2
Trivial name: water-soluble β-galactoglucofurannan (SVPS2)
Contained glycoepitopes: IEDB_136044,IEDB_136045,IEDB_137472,IEDB_141794,IEDB_142489,IEDB_144562,IEDB_146664,IEDB_152214,IEDB_174333,IEDB_190606,IEDB_983931,SB_165,SB_166,SB_187,SB_195,SB_7,SB_86,SB_88

Methods: 13C NMR, 1H NMR, methylation, NMR-2D, GC-MS, sugar analysis, Western blotting, FTIR, HPLC, cytokine analysis, extraction, statistical analysis, SEC-MALLS, cell viability assay, antitumor activity assay, AFM, ion exchange chromatography, apoptosis assays, immunomodulatory activity analysis, wild cultivation
Comments, role: presumed main structure of SVPS2; NMR temperature was not specified; published NMR spectra were removed by CSDB staff due to erroneous assignment.

NCBI Taxonomy refs (TaxIDs): 175686
Show glycosyltransferases

NMR conditions: in D2O [as TSV]

1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5	H6
5,5,5,5,5	bDGlcf	5.20	4.16	4.12	4.14	3.96	3.74-3.81
5,5,5,5,6	bDGalp	4.53	3.42	3.67	3.52	3.78	3.91
5,5,5,5	bDGlcf	5.03	4.14	4.12	4.12	3.89	3.89-4.22
5	bDGlcf	5.20	4.16	4.12	4.14	3.96	3.74-3.81
5,5,5,6	bDGlcf	5.25	4.16	4.08	4.08	3.82	3.67-3.81
5,5,5	bDGlcf	5.03	4.14	4.12	4.12	3.89	3.89-4.22
5,5,6	aLFucp	5.00	3.92	3.54	3.52	4.08	1.25
5,5	bDGlcf	5.03	4.14	4.12	4.12	3.89	3.89-4.22
	bDGlcf

¹H NMR data:

Linkage	Residue	H1	H2	H3	H4	H5	H6
5,5,5,5,5	bDGlcf	5.20	4.16	4.12	4.14	3.96	3.74 3.81
5,5,5,5,6	bDGalp	4.53	3.42	3.67	3.52	3.78	3.91
5,5,5,5	bDGlcf	5.03	4.14	4.12	4.12	3.89	3.89 4.22
5	bDGlcf	5.20	4.16	4.12	4.14	3.96	3.74 3.81
5,5,5,6	bDGlcf	5.25	4.16	4.08	4.08	3.82	3.67 3.81
5,5,5	bDGlcf	5.03	4.14	4.12	4.12	3.89	3.89 4.22
5,5,6	aLFucp	5.00	3.92	3.54	3.52	4.08	1.25
5,5	bDGlcf	5.03	4.14	4.12	4.12	3.89	3.89 4.22
	bDGlcf

¹³C NMR data: present in publication with incorrect assignment

There is only one chemically distinct structure:

[*]O[C@H](CO)[C@H]1O[C@@H](O[C@H](CO)[C@H]2O[C@@H](O[C@H](CO)[C@H]3O[C@@H](O[C@H](CO)[C@H]4O[C@@H](O[C@H](CO[C@@H]5O[C@H](CO)[C@H](O)[C@H](O)[C@H]5O)[C@H]5O[C@@H](O[C@H](CO[C@@H]6O[C@H](CO)[C@H](O)[C@H](O)[C@H]6O)[C@H]6O[C@@H](O[C@H](CO[C@@H]7O[C@H](CO)[C@H](O)[C@H](O)[C@H]7O)[C@H]7O[C@@H](O[C@H](CO[C@@H]8O[C@H]([C@H](O)CO)[C@H](O)[C@H]8O)[C@H]8O[C@@H](O[C@H](CO[C@@H]9O[C@@H](C)[C@@H](O)[C@@H](O)[C@@H]9O)[C@H]9O[C@@H](O[C@H](CO)[C@H]%10O[C@@H](O[C@H](CO)[C@H]%11O[C@@H](O[C@H](CO)[C@H]%12O[C@@H](O[C@H](CO)[C@H]%13O[C@@H]([*])[C@H](O)[C@H]%13O)[C@H](O)[C@H]%12O)[C@H](O)[C@H]%11O)[C@H](O)[C@H]%10O)[C@H](O)[C@H]9O)[C@H](O)[C@H]8O)[C@H](O)[C@H]7O)[C@H](O)[C@H]6O)[C@H](O)[C@H]5O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)[C@H](O)[C@H]2O)[C@H](O)[C@H]1O

SVG MW SMILES 3D mol Ionize