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Taxonomic group: fungi / Ascomycota (Phylum: Ascomycota)
Organ / tissue: cell wall of fission yeast

NCBI PubMed ID: 36058798
Publication DOI: 10.1016/j.jbiosc.2022.07.014
Journal NLM ID: 100888800
Publisher: Osaka, Japan, Amsterdam, The Netherlands: Society for Bioscience and Bioengineering
Correspondence: K. Takegawa <takegawa

agr.kyushu-u.ac.jp>
Institutions: Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan, Faculty of Science and Engineering, Department of Life Science, Setsunan University, 17-8 Ikedanaka-machi, Neyagawa, Osaka 572-8508, Japan, Department of Infection and Host Defense, Tohoku Medical and Pharmaceutical University, 4-4-1 Komatsujima, Sendai, Miyagi 981-8558, Japan

Schizosaccharomyces japonicus is a dimorphic yeast, transiting between unicellular and hyphal growth. The glycoproteins of fission yeast contain, in addition to mannose (Man), a large number of galactose (Gal) residues. Previously, we reported that the cell-surface O-glycans of S. japonicus comprise mainly tri-saccharides (Gal-Man-Man) as a main component, in contrast to the tetra-saccharides observed in other Schizosaccharomyces species. Here we have investigated the function of cell-surface Gal residues in S. japonicus. Because disruption of gms1+, encoding the UDP-Gal transporter required for galactomannan synthesis, abolishes cell-surface galactosylation in Schizosaccharomyces pombe, we constructed a deletion mutant of the homologous gene in S. japonicus gms1Δ [gms1 (S.j)] and determined the N- and O-linked oligosaccharide structures present on the cell surface. Disruption of gms1 (S.j) resulted in a complete lack of Gal on the cell surface, indicating that Gms1 plays an essential role in supplying UDP-Gal from the cytoplasm to the Golgi lumen. Analytical microscopy of gms1Δ demonstrated that the lack of cell-surface Gal did not affect cell growth or morphology during vegetative growth. However, hyphal development was blocked in gms1Δ, even in the presence of the topoisomerase I inhibitor camptothecin, which is known to induce hyphal differentiation in wild-type S. japonicus. Collectively, these findings show that Gal-containing oligosaccharides are required for cell wall integrity during filamentous growth in S. japonicus.

Galactomannan, O-glycans, fission yeast, hyphal differentation, UDP-Gal transporter

Structure type: oligomer
Location inside paper: Fig. 2A
Aglycon: Asn
Trivial name: N-linked galactomannan
Compound class: cell-surface polysaccharide
Contained glycoepitopes: IEDB_123886,IEDB_130701,IEDB_135813,IEDB_136104,IEDB_137340,IEDB_137485,IEDB_140116,IEDB_140942,IEDB_141793,IEDB_141807,IEDB_141828,IEDB_141829,IEDB_141830,IEDB_141831,IEDB_143632,IEDB_144983,IEDB_151079,IEDB_151531,IEDB_152206,IEDB_153212,IEDB_153220,IEDB_153762,IEDB_153763,IEDB_164046,IEDB_164174,IEDB_187201,IEDB_187238,IEDB_187239,IEDB_187240,IEDB_429156,IEDB_540671,IEDB_548907,IEDB_76933,IEDB_857734,IEDB_983930,SB_136,SB_191,SB_196,SB_197,SB_198,SB_33,SB_44,SB_53,SB_67,SB_72,SB_73,SB_74,SB_77,SB_85

Methods: 1H NMR, PCR, DNA techniques, HPLC, microscopy, cloning, bioinformatic analysis, pyridylamination, lectin staining, hyphal formation

Related record ID(s): 51618
NCBI Taxonomy refs (TaxIDs): 4897
Show glycosyltransferases

There are 4 chemically distinct structures. Please, select:

CC(=O)N[C@H]1C(OC2O[C@H](CO)[C@@H](O[C@@H]3O[C@H](CO[C@H]4O[C@H](CO[C@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]5O[C@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]5O)[C@@H](O)[C@H](O[C@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]5O[C@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]5O)[C@@H]4O)[C@@H](O)[C@H](O[C@H]4O[C@H](CO[C@H]5O[C@H](CO[C@H]6O[C@H](CO[C@H]7O[C@H](CO[C@H]8O[C@H](CO[C@H]9O[C@H](CO[C@H]%10O[C@H](CO[C@H]%11O[C@H](CO[C@H]%12O[C@H](CO[C@H]%13O[C@H](CO[C@H]%14O[C@H](CO[C@H]%15O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]%15O)[C@@H](O)[C@H](O)[C@@H]%14O)[C@@H](O)[C@H](O)[C@@H]%13O)[C@@H](O)[C@H](O)[C@@H]%12O)[C@@H](O)[C@H](O)[C@@H]%11O)[C@@H](O)[C@H](O)[C@@H]%10O)[C@@H](O)[C@H](O)[C@@H]9O)[C@@H](O)[C@H](O)[C@@H]8O)[C@@H](O)[C@H](O)[C@@H]7O)[C@@H](O)[C@H](O)[C@@H]6O)[C@@H](O)[C@H](O)[C@@H]5O)[C@@H](O)[C@H](O)[C@@H]4O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O)[C@@H]3O)[C@H](O)[C@H]2NC(C)=O)O[C@H](CO)[C@@H](O)[C@@H]1O

SVG MW SMILES 3D mol Ionize

Warning = sub-repeat ending at aDManp: variable unit count (50-100) was assumed to be 50
Warning = sub-repeat ending at aDManp: large unit count (50) was reduced to 5