Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
The structure was elucidated in this paperNCBI PubMed ID: 36590213Publication DOI: 10.3389/fnut.2022.1092582Journal NLM ID: 101642264Publisher: Lausanne, Switzerland: Frontiers Media S.A.
Correspondence: D. Wang <wangdawei
jlau.edu.cn>; T. Liu <liutingting
jlau.edu.cn>
Institutions: School of Food Science and Engineering, Jilin Agricultural University, Changchun, China, Scientific Research Base of Edible Mushroom Processing Technology Integration, Ministry of Agriculture and Rural Affairs, Changchun, China, Engineering Research Center of Grain Deep-Processing and High-Efficiency Utilization of Jilin, Changchun, China, Key Laboratory of Technological Innovations for Grain Deep-Processing and High-Efficiency Utilization of By-Products of Jilin, Changchun, China
Stropharia rugosoannulata is a widely grown edible mushroom with a high nutritional value. S. rugosoannulata polysaccharides is one of the most important bioactive components of S. rugosoannulata and has a wide range of activities. A S. rugosoannulata polysaccharides, named SRF-3, was derived from the S. rugosoannulata extraction by freeze-thaw combine with hot water extraction method, then prepareed with DEAE-cellulose column and Sephacryl S-200 HR gel column, and its hypolipidemic activity was determined. The structural characteristics of SRF-3 were analyzed by infrared spectral scanning (FT-IR), ultra-high performance liquid chromatography (UHPLC), acid hydrolysis, methylation analysis, nuclear magnetic resonance (NMR), and Gas Chromatography-Mass Spectrometer (GC-MS). SRF-3 is composed of mannose, galactose, methyl galactose and fructose with ratios of 16, 12, 58 and 12, respectively. In addition, the average relative molecular mass of SRF-3 is approximately 24 kDa. The main chain of SRF-3 is mainly composed of repeating α-D-1,6-Galp and α-D-1,6-Me-Galp units, with branches in the O-2 position of Gal. The structure is presumed to be a mannogalactan, with a small amount of t-β-D-Manp present as a side chain. Hypolipidemic activity assay showed that SRF-3 had good antioxidant and hypolipidemic effects in vitro, suggesting that SRF-3 have potential application in reducing liver fat accumulation.
structure, polysaccharides, mushroom, antioxidation, hypolipidemic, Stropharia rugosoannulata
Structure type: fragment of a bigger structure ; 24000
Location inside paper: abstract, table 3, p. 1092582-8
Trivial name: water-soluble polysaccharide SRF-3
Contained glycoepitopes: IEDB_134624,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_151528,IEDB_190606,SB_163,SB_7
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GC-MS, sugar analysis, acid hydrolysis, FTIR, extraction, statistical analysis, antioxidant activities, HPGPC, radical scavenging assay, UHPLC, hypolipidemic activities in vitro assays
Comments, role: fragment of the main chain of SRF-3
Related record ID(s): 51748
NCBI Taxonomy refs (TaxIDs): 68746
Show glycosyltransferases
NMR conditions: in D2O at 293 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
6 aDGalp 96.79 68.45 67.22 69.33 67.76 66.21
3 Me 54.98
aDGalp 96.79 68.45 77.72 65.44 67.76 66.21
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
6 aDGalp 4.93 3.84 3.97 4.03 4.15 3.63-3.85
3 Me 3.38
aDGalp 4.93 3.84 3.51 4.25 4.15 3.63-3.85
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
6 aDGalp 96.79/4.93 68.45/3.84 67.22/3.97 69.33/4.03 67.76/4.15 66.21/3.63-3.85
3 Me 54.98/3.38
aDGalp 96.79/4.93 68.45/3.84 77.72/3.51 65.44/4.25 67.76/4.15 66.21/3.63-3.85
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 6 | aDGalp | 4.93 | 3.84 | 3.97 | 4.03 | 4.15 | 3.63
3.85 |
| 3 | Me | 3.38 | |
| | aDGalp | 4.93 | 3.84 | 3.51 | 4.25 | 4.15 | 3.63
3.85 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 6 | aDGalp | 96.79 | 68.45 | 67.22 | 69.33 | 67.76 | 66.21 |
| 3 | Me | 54.98 | |
| | aDGalp | 96.79 | 68.45 | 77.72 | 65.44 | 67.76 | 66.21 |
|
There is only one chemically distinct structure:
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
The structure was elucidated in this paperNCBI PubMed ID: 36590213Publication DOI: 10.3389/fnut.2022.1092582Journal NLM ID: 101642264Publisher: Lausanne, Switzerland: Frontiers Media S.A.
Correspondence: D. Wang <wangdawei
jlau.edu.cn>; T. Liu <liutingting
jlau.edu.cn>
Institutions: School of Food Science and Engineering, Jilin Agricultural University, Changchun, China, Scientific Research Base of Edible Mushroom Processing Technology Integration, Ministry of Agriculture and Rural Affairs, Changchun, China, Engineering Research Center of Grain Deep-Processing and High-Efficiency Utilization of Jilin, Changchun, China, Key Laboratory of Technological Innovations for Grain Deep-Processing and High-Efficiency Utilization of By-Products of Jilin, Changchun, China
Stropharia rugosoannulata is a widely grown edible mushroom with a high nutritional value. S. rugosoannulata polysaccharides is one of the most important bioactive components of S. rugosoannulata and has a wide range of activities. A S. rugosoannulata polysaccharides, named SRF-3, was derived from the S. rugosoannulata extraction by freeze-thaw combine with hot water extraction method, then prepareed with DEAE-cellulose column and Sephacryl S-200 HR gel column, and its hypolipidemic activity was determined. The structural characteristics of SRF-3 were analyzed by infrared spectral scanning (FT-IR), ultra-high performance liquid chromatography (UHPLC), acid hydrolysis, methylation analysis, nuclear magnetic resonance (NMR), and Gas Chromatography-Mass Spectrometer (GC-MS). SRF-3 is composed of mannose, galactose, methyl galactose and fructose with ratios of 16, 12, 58 and 12, respectively. In addition, the average relative molecular mass of SRF-3 is approximately 24 kDa. The main chain of SRF-3 is mainly composed of repeating α-D-1,6-Galp and α-D-1,6-Me-Galp units, with branches in the O-2 position of Gal. The structure is presumed to be a mannogalactan, with a small amount of t-β-D-Manp present as a side chain. Hypolipidemic activity assay showed that SRF-3 had good antioxidant and hypolipidemic effects in vitro, suggesting that SRF-3 have potential application in reducing liver fat accumulation.
structure, polysaccharides, mushroom, antioxidation, hypolipidemic, Stropharia rugosoannulata
Structure type: fragment of a bigger structure ; 24000
Location inside paper: abstract, table 3, p. 1092582-8
Trivial name: water-soluble polysaccharide SRF-3
Contained glycoepitopes: IEDB_134624,IEDB_136906,IEDB_137472,IEDB_137485,IEDB_141794,IEDB_144983,IEDB_151528,IEDB_152206,IEDB_190606,IEDB_983930,SB_163,SB_44,SB_7,SB_72
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GC-MS, sugar analysis, acid hydrolysis, FTIR, extraction, statistical analysis, antioxidant activities, HPGPC, radical scavenging assay, UHPLC, hypolipidemic activities in vitro assays
Comments, role: fragment of the main chain of SRF-3
Related record ID(s): 51622
NCBI Taxonomy refs (TaxIDs): 68746
Show glycosyltransferases
NMR conditions: in D2O at 293 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
2 bDManp 100.65 70.22 71.83 65.73 75.17 59.99
aDGalp 97.35 75.87 67.22 69.33 67.76 64.01
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
2 bDManp 4.75 4.05 3.61 3.54 3.34 3.69-3.86
aDGalp 5.08 3.91 3.97 4.05 4.15 3.51-3.85
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
2 bDManp 100.65/4.75 70.22/4.05 71.83/3.61 65.73/3.54 75.17/3.34 59.99/3.69-3.86
aDGalp 97.35/5.08 75.87/3.91 67.22/3.97 69.33/4.05 67.76/4.15 64.01/3.51-3.85
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 2 | bDManp | 4.75 | 4.05 | 3.61 | 3.54 | 3.34 | 3.69
3.86 |
| | aDGalp | 5.08 | 3.91 | 3.97 | 4.05 | 4.15 | 3.51
3.85 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 2 | bDManp | 100.65 | 70.22 | 71.83 | 65.73 | 75.17 | 59.99 |
| | aDGalp | 97.35 | 75.87 | 67.22 | 69.33 | 67.76 | 64.01 |
|
There is only one chemically distinct structure:
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