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Taxonomic group: fungi / Basidiomycota (Phylum: Basidiomycota)
Organ / tissue: mycelium

The structure was elucidated in this paper
NCBI PubMed ID: 35487375
Publication DOI: 10.1016/j.ijbiomac.2022.04.154
Journal NLM ID: 7909578
Publisher: Butterworth-Heinemann
Correspondence: Z. Zhu <zhyuanzhu

tust.edu.cn>
Institutions: Key Laboratory of Food Nutrition and Safety, Ministry of Education-Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin, China, State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin, China, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, China, College of Public Health, Zunyi Medical University, Guizhou, China

The crude polysaccharide LDP was extracted from mycelia of Lactarius deliciosus Gray and then purified by DEAE-52 cellulose and Sephadex G-200 to obtain a novel polysaccharide named LDP-CP. LDP-CP was mainly composed of mannose, glucose and galactose with an average molecular weight of 2.33 × 103 kDa. The structure of LDP-CP was determined by FT-IR, methylation and NMR analysis, and the results showed that the sugar linkage units of LDP-CP were composed of (1 → 3)-linked β-D-Manp, (1→2,4)-linked α-D-Manp, (1→)-linked α-D-Manp, (1→4)-linked β-D-Glcp, (1→2)-linked β-D-Manp, (1→4,6)-linked α-D-Manp, (1→4)-linked α-D-Galp, (1→2,3)-linked α-D-Glcp and (1→)-linked α-D-Glcf. The protective effects of LDP and LDP-CP on PC12 cells against H2O2-induced oxidative injury were exhibited by enhancing cell viability and morphological protection. The improvement to the level of LDH, SOD and GSH further indicated that LDP and LDP-CP had ability to alleviate H2O2-induced oxidative damage on PC12 cells. The polysaccharides in Lactarius deliciosus Gray mycelia exhibited the great advantages in the management of oxidative toxicity, which indicated that the polysaccharides can be further developed in application of natural functional food source.

polysaccharide, Lactarius deliciosus Gray, oxidative damage

Structure type: structural motif or average structure ; 2330000
Location inside paper: Fig. 4
Trivial name: polysaccharide LDP-CP
Contained glycoepitopes: IEDB_115576,IEDB_130701,IEDB_136104,IEDB_136906,IEDB_137472,IEDB_137485,IEDB_140116,IEDB_141794,IEDB_142488,IEDB_143632,IEDB_144983,IEDB_144987,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_152206,IEDB_190606,IEDB_232584,IEDB_232585,IEDB_76933,IEDB_983930,IEDB_983931,SB_136,SB_192,SB_196,SB_197,SB_31,SB_44,SB_67,SB_7,SB_72

Methods: 13C NMR, 1H NMR, methylation, GC-MS, sugar analysis, FTIR, HPLC, extraction, optical rotation measurement, statistical analysis, cell viability assay, SEM, ion exchange chromatography, tissue isolation, oxidative stress, DrawGlycan-SNFG
Biological activity: The protective effects of LDP and LDP-CP (at the concentration of 3.2 mg/mL) against oxidative injury in H2O2-induced PC12 cells were performed by increasing cell viability by over 70%, morphological protection, and the improvement to the activity of LDH and SOD and the level of GSH.
Comments, role: The deduced structure of LDP-CP; NMR spectra were recorded at room temperature.

NCBI Taxonomy refs (TaxIDs): 55514
Show glycosyltransferases

NMR conditions: in D2O

¹H NMR data: present in publication

¹³C NMR data: present in publication

There is only one chemically distinct structure:

[*]O[C@@H]1[C@@H](O[C@H]2[C@@H](O)[C@H](O)[C@@H](CO[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]3O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]3O)O[C@@H]2O[C@@H]2[C@@H](CO[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]3O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]3O)O[C@H](O[C@@H]3[C@@H](CO[C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O)O[C@H](O[C@@H]4[C@@H](CO[C@@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]5O[C@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]5O)O[C@H](O[C@@H]5[C@@H](CO[C@@H]6O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]6O[C@H]6O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]6O)O[C@H](O[C@H]6[C@@H](CO)O[C@H](O[C@H]7[C@@H](CO)O[C@H](O[C@H]8[C@@H](CO)O[C@H](O[C@H]9[C@@H](CO)O[C@H](O[C@H]%10[C@@H](CO)O[C@H](O[C@@H]%11[C@@H](CO)O[C@@H](O[C@@H]%12[C@@H](CO)O[C@@H](O[C@@H]%13[C@@H](CO)O[C@@H](O[C@@H]%14[C@@H](CO)O[C@@H](O[C@@H]%15[C@@H](CO)O[C@@H](O[C@@H]%16[C@@H](CO)O[C@H](O[C@@H]%17[C@@H](O[C@H]%18O[C@H]([C@H](O)CO)[C@H](O)[C@H]%18O)[C@H](O)[C@@H](CO)O[C@@H]%17O[C@@H]%17[C@@H](O[C@H]%18O[C@H]([C@H](O)CO)[C@H](O)[C@H]%18O)[C@H](O)[C@@H](CO)O[C@@H]%17O[C@@H]%17[C@@H](O[C@H]%18O[C@H]([C@H](O)CO)[C@H](O)[C@H]%18O)[C@H](O)[C@@H](CO)O[C@@H]%17O[C@@H]%17[C@@H](O[C@H]%18O[C@H]([C@H](O)CO)[C@H](O)[C@H]%18O)[C@H](O)[C@@H](CO)O[C@@H]%17[*])[C@H](O)[C@H]%16O[C@H]%16O[C@H]([C@H](O)CO)[C@H](O)[C@H]%16O)[C@H](O)[C@H]%15O)[C@H](O)[C@H]%14O)[C@H](O)[C@H]%13O)[C@H](O)[C@H]%12O)[C@H](O)[C@H]%11O)[C@H](O)[C@H]%10O)[C@H](O)[C@H]9O)[C@H](O)[C@H]8O)[C@H](O)[C@H]7O)[C@H](O)[C@H]6O)[C@@H](O)[C@H]5O)[C@@H](O)[C@H]4O)[C@@H](O)[C@H]3O)[C@@H](O)[C@H]2O)O[C@H](CO)[C@@H](O[C@@H]2O[C@H](CO)[C@@H](O)[C@H](O[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O[C@@H]4O[C@H](CO)[C@@H](O)[C@H](O[C@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]5O)[C@@H]4O)[C@@H]3O)[C@@H]2O)[C@@H]1O

SVG MW SMILES 3D mol Ionize

Warning = sub-repeat ending at bDGlcp: large unit count (16) was reduced to 5
Warning = sub-repeat ending at aDGalp: large unit count (28) was reduced to 5
Warning = sub-repeat ending at aDManp: large unit count (9) was reduced to 5