Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: mushroom powder
Publication DOI: 10.1016/j.colsurfa.2022.128684Journal NLM ID: 9891752Publisher: Amsterdam, New York: Elsevier
Correspondence: M.E.N.P. Ricardo <elenir.ribeiro
ufc.br>; N.M.P.S. Ricardo <naricard
ufc.br>; S.D.A. Soares <sas
ufc.br>
Institutions: Laboratory of Polymers and Materials Innovation, Department of Organic and Inorganic Chemistry, Federal University of Ceará, Campus of Pici, Zip Code: 60440-900 Fortaleza, CE, Brazil, Center for Pharmaceutical and Cosmetic Studies, Department of Pharmacy, Federal University of Ceará, Porangabussu Campus, Zip Code: 60430-370 Fortaleza, CE, Brazil
This study evaluated the effects of polysaccharides isolated from Lentinus edodes Berk and Agaricus blazei Murill mushrooms on the gelation process of the Pluronic® F127 (F127) copolymer. The structural characterization of these biopolymers suggested the isolation of an β-glucan L. edodes Berk (LEB) and a proteoglycan complex A. blazei Murill (ABM). Both polysaccharides showed good rheological properties for developing pharmaceutical formulations with antioxidant activity and low cytotoxicity in human neutrophils. The characterization of the gels formed from the mixture of polysaccharides with aqueous solutions of F127 showed that the presence of these biopolymers reduces the temperature range in which F127 remains in the gel phase (G’ > G’’). Furthermore, the thermoresponsive behavior of F127 was not lost, which favors the development of hydrogels with potential for topical use.
polysaccharide, Agaricus blazei, Lentinus edodes, copolymer, Pluronic F127
Structure type: fragment of a bigger structure ; 3350000
Location inside paper: p. 128684-5, table 1, ABM
Trivial name: (1,4)-α-glucan, proteoglycan complex, ABM
Contained glycoepitopes: IEDB_140629,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_420417,IEDB_420418,IEDB_420421,IEDB_857742,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, FTIR, GPC, extraction, statistical analysis, antioxidant activities, cytotoxicity assay, rheological study, AFM, intrinsic viscosity measurement, F127-polysaccharides gels preparation
Comments, role: NMR temperature was not specified
Related record ID(s): 51790
NCBI Taxonomy refs (TaxIDs): 307931
Show glycosyltransferases
NMR conditions: in D2O
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
aDGlcp 99.5 71.4 75.1 78.5 70.0 59.0
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
aDGlcp 5.3 3.4 3.6 3.8 4.0 4.4
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
aDGlcp 99.5/5.3 71.4/3.4 75.1/3.6 78.5/3.8 70.0/4.0 59.0/4.4
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| | aDGlcp | 5.3 | 3.4 | 3.6 | 3.8 | 4.0 | 4.4 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | aDGlcp | 99.5 | 71.4 | 75.1 | 78.5 | 70.0 | 59.0 |
|
There is only one chemically distinct structure:
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: mushroom powder
Publication DOI: 10.1016/j.colsurfa.2022.128684Journal NLM ID: 9891752Publisher: Amsterdam, New York: Elsevier
Correspondence: M.E.N.P. Ricardo <elenir.ribeiro
ufc.br>; N.M.P.S. Ricardo <naricard
ufc.br>; S.D.A. Soares <sas
ufc.br>
Institutions: Laboratory of Polymers and Materials Innovation, Department of Organic and Inorganic Chemistry, Federal University of Ceará, Campus of Pici, Zip Code: 60440-900 Fortaleza, CE, Brazil, Center for Pharmaceutical and Cosmetic Studies, Department of Pharmacy, Federal University of Ceará, Porangabussu Campus, Zip Code: 60430-370 Fortaleza, CE, Brazil
This study evaluated the effects of polysaccharides isolated from Lentinus edodes Berk and Agaricus blazei Murill mushrooms on the gelation process of the Pluronic® F127 (F127) copolymer. The structural characterization of these biopolymers suggested the isolation of an β-glucan L. edodes Berk (LEB) and a proteoglycan complex A. blazei Murill (ABM). Both polysaccharides showed good rheological properties for developing pharmaceutical formulations with antioxidant activity and low cytotoxicity in human neutrophils. The characterization of the gels formed from the mixture of polysaccharides with aqueous solutions of F127 showed that the presence of these biopolymers reduces the temperature range in which F127 remains in the gel phase (G’ > G’’). Furthermore, the thermoresponsive behavior of F127 was not lost, which favors the development of hydrogels with potential for topical use.
polysaccharide, Agaricus blazei, Lentinus edodes, copolymer, Pluronic F127
Structure type: fragment of a bigger structure ; 954000, 3350000
Location inside paper: p. 128684-5, table 1, LEB, ABM
Trivial name: polysaccharide LEB, proteoglycan complex, ABM
Compound class: β-glucan
Contained glycoepitopes: IEDB_142488,IEDB_144998,IEDB_146664,IEDB_158538,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, FTIR, GPC, extraction, statistical analysis, antioxidant activities, cytotoxicity assay, rheological study, AFM, intrinsic viscosity measurement, F127-polysaccharides gels preparation
Comments, role: NMR temperature was not specified
Related record ID(s): 51649
NCBI Taxonomy refs (TaxIDs): 5353,
307931
Show glycosyltransferases
NMR conditions: in D2O
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
aDGlcp 102.7 72.7 75.3 69.6 74.6 67.9
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
aDGlcp 4.6 3.3 3.5 3.7 3.9 4.2
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
aDGlcp 102.7/4.6 72.7/3.3 75.3/3.5 69.6/3.7 74.6/3.9 67.9/4.2
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| | aDGlcp | 4.6 | 3.3 | 3.5 | 3.7 | 3.9 | 4.2 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | aDGlcp | 102.7 | 72.7 | 75.3 | 69.6 | 74.6 | 67.9 |
|
There is only one chemically distinct structure:
Found 
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