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Type K82 capsular polysaccharide (CPS) was isolated from Acinetobacter baumannii LUH5534. The structure of a linear tetrasaccharide repeating unit of the CPS was established by sugar analysis along with one- and two-dimensional 1H and 13C NMR spectroscopy. Proteins encoded by the KL82 capsule gene cluster in the genome of LUH5534 were assigned to roles in the synthesis of the K82 CPS. In particular, functions were assigned to two new glycosyltransferases (Gtr152 and Gtr153) and a novel pyruvyltransferase, Ptr5, responsible for the synthesis of d-galactose 4,6-(R)-pyruvic acid acetal.

Acinetobacter baumannii, capsular polysaccharide structure, biotechnology, Pyruvic acid acetal, K locus, genetics of capsule biosynthesis

NCBI PubMed ID: 30200867
Publication DOI: 10.1134/S0006297918070064
Journal NLM ID: 0376536
Publisher: Nauka/Interperiodica
Correspondence: yknirel

gmail.com; nastia-kasimova979797

mail.ru
Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, 142279, Russia, School of Molecular Bioscience, The University of Sydney, Sydney, NSW 2006, Australia, Higher Chemical College of the Russian Academy of Sciences, D. I. Mendeleev University of Chemical Technology of Russia, Moscow, Russia, Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, QLD 4059, Australia, Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, 141700, Russia, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, GLC, mild acid hydrolysis, GPC, dialysis, bioinformatic analysis (BLASTp)