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1. (CSDB ID: 51652) | report error |
| a-D-Manp-(1-2)-a-D-Manp-(1-2)-a-D-Manp-(1-3)-+ | a-D-Manp-(1-2)-a-D-Manp-(1-6)-+ | | | a-D-Manp-(1-2)-a-D-Manp-(1-3)-a-D-Manp-(1-6)-b-D-Manp-(1-4)-b-D-GlcpNAc-(1-4)-b-D-GlcpNAc | Show graphically |
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Neurospora crassa A Δoch-1
(Ancestor NCBI TaxID 5141,
species name lookup)
buffalo.edu>The formation of a cell wall is vital for the survival and growth of a fungal cell. Fungi express members of the GH76 family of α-1,6-mannanases which play an important role in cell wall biogenesis. In this report we characterize the Neurospora crassa DFG-5 α-1,6-mannanase and demonstrate that it binds to the α-1,6-mannose backbone of an N-linked galactomannan found on cell wall glycoproteins. We show that DFG-5 has an enzymatic activity and provide evidence that it processes the α-1,6-mannose backbone of the N-linked galactomannan. Site-directed mutagenesis and complementation experiments show that D116 and D117 are located at the DFG-5 active site. D76 and E130, which are located in a groove on the opposite side of the protein, are also important for enzyme function. Cell wall glycoproteins co-purify with DFG-5 demonstrating a specific association between DFG-5 and cell wall glycoproteins. DFG-5 is able to discriminate between cell wall and secreted glycoproteins, and does not bind to the N-linked galactomannans present on secreted glycoproteins. DFG-5 plays a key role in targeting extracellular glycoproteins to their final destinations. By processing the galactomannans on cell wall proteins, DFG-5 targets them for cell wall incorporation by lichenin transferases. The N-linked galactomannans on secreted proteins are not processed by DFG-5, which targets these proteins for release into the extracellular medium.
Galactomannan, protein secretion, cell wall biosynthesis, DFG5, Extracellular protein targeting, GH76 α-1, 6-mannanase
Structure type: oligomer|
2. (CSDB ID: 51815) | report error |
| a-D-Manp-(1-2)-a-D-Manp-(1-6)-+ | a-D-Manp-(1-2)-a-D-Manp-(1-3)-a-D-Manp-(1-6)-+ | D-Galf-(1-2)-+ | | | D-Galf-(1-2)-+ | | | | | D-Galf-(1-2)-+ | | | | | | | D-Galf-(1-2)-+ | | | a-D-Manp-(1-2)-a-D-Manp-(1-2)-+ | | | | | | | D-Galf-(1-2)-a-D-Manp-(1-6)-a-D-Manp-(1-6)-a-D-Manp-(1-6)-a-D-Manp-(1-6)-a-D-Manp-(1-6)-a-D-Manp-(1-6)-a-D-Manp-(1-6)-a-D-Manp-(1-3)-b-D-Manp-(1-4)-b-D-GlcpNAc-(1-4)-b-D-GlcpNAc | Show graphically |
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Neurospora crassa A Δdfg-5/Δdcw-1
(Ancestor NCBI TaxID 5141,
species name lookup)
buffalo.edu>The formation of a cell wall is vital for the survival and growth of a fungal cell. Fungi express members of the GH76 family of α-1,6-mannanases which play an important role in cell wall biogenesis. In this report we characterize the Neurospora crassa DFG-5 α-1,6-mannanase and demonstrate that it binds to the α-1,6-mannose backbone of an N-linked galactomannan found on cell wall glycoproteins. We show that DFG-5 has an enzymatic activity and provide evidence that it processes the α-1,6-mannose backbone of the N-linked galactomannan. Site-directed mutagenesis and complementation experiments show that D116 and D117 are located at the DFG-5 active site. D76 and E130, which are located in a groove on the opposite side of the protein, are also important for enzyme function. Cell wall glycoproteins co-purify with DFG-5 demonstrating a specific association between DFG-5 and cell wall glycoproteins. DFG-5 is able to discriminate between cell wall and secreted glycoproteins, and does not bind to the N-linked galactomannans present on secreted glycoproteins. DFG-5 plays a key role in targeting extracellular glycoproteins to their final destinations. By processing the galactomannans on cell wall proteins, DFG-5 targets them for cell wall incorporation by lichenin transferases. The N-linked galactomannans on secreted proteins are not processed by DFG-5, which targets these proteins for release into the extracellular medium.
Galactomannan, protein secretion, cell wall biosynthesis, DFG5, Extracellular protein targeting, GH76 α-1, 6-mannanase
Structure type: oligomer|
3. (CSDB ID: 51816) | report error |
| a-D-Manp-(1-2)-a-D-Manp-(1-6)-+ | a-D-Manp-(1-2)-a-D-Manp-(1-3)-a-D-Manp-(1-6)-+ | a-D-Manp-(1-6)-a-D-Manp-(1-6)-+ | | | a-D-Manp-(1-2)-a-D-Manp-(1-2)-a-D-Manp-(1-3)-b-D-Manp-(1-4)-b-D-GlcpNAc-(1-4)-b-D-GlcpNAc | Show graphically |
|
Show legend Show as text |
Neurospora crassa A Δgel-1/Δgel-2/Δgel-5
(Ancestor NCBI TaxID 5141,
species name lookup)
buffalo.edu>The formation of a cell wall is vital for the survival and growth of a fungal cell. Fungi express members of the GH76 family of α-1,6-mannanases which play an important role in cell wall biogenesis. In this report we characterize the Neurospora crassa DFG-5 α-1,6-mannanase and demonstrate that it binds to the α-1,6-mannose backbone of an N-linked galactomannan found on cell wall glycoproteins. We show that DFG-5 has an enzymatic activity and provide evidence that it processes the α-1,6-mannose backbone of the N-linked galactomannan. Site-directed mutagenesis and complementation experiments show that D116 and D117 are located at the DFG-5 active site. D76 and E130, which are located in a groove on the opposite side of the protein, are also important for enzyme function. Cell wall glycoproteins co-purify with DFG-5 demonstrating a specific association between DFG-5 and cell wall glycoproteins. DFG-5 is able to discriminate between cell wall and secreted glycoproteins, and does not bind to the N-linked galactomannans present on secreted glycoproteins. DFG-5 plays a key role in targeting extracellular glycoproteins to their final destinations. By processing the galactomannans on cell wall proteins, DFG-5 targets them for cell wall incorporation by lichenin transferases. The N-linked galactomannans on secreted proteins are not processed by DFG-5, which targets these proteins for release into the extracellular medium.
Galactomannan, protein secretion, cell wall biosynthesis, DFG5, Extracellular protein targeting, GH76 α-1, 6-mannanase
Structure type: oligomer| New query | Export IDs | Home | Help |
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