Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting bodies
NCBI PubMed ID: 36007700Publication DOI: 10.1016/j.ijbiomac.2022.08.128Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: Y. Liu <aliu-1980
163.com>; J. Zhang <syja16
saas.sh.cn>
Institutions: Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, Shanghai 201403, China, Engineering Research Centre of Pharmaceutical Process Chemistry, Ministry of Education, Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China
Microwave-assisted degradation of β-(1→3,1→6)-D-glucan from Ganoderma lucidum and correlated immunoregulatory activities were investigated in this study. The optimal temperature and degradation time for microwave hydrothermal hydrolysis were 140 °C and 40 min, respectively. Under these conditions, a high yield of degradation rate (98.4 %) and abundant β-oligosaccharide products (GLOS) with different degrees of polymerization (DP 2-24) were obtained. Four fractions including F1 (DP 2-8), F2 (DP 6-19), F3 (DP 8-24) and F4 (high DPs) with different average ratios of β-(1→3) to β-(1→6)-linked glucose units were isolated from GLOS. The structures of oligosaccharides with DP (2-6) in F1 were identified as linear β-(1→3)-linked glucooligosaccharides without or with β-(1→6)-linked glucose residues based on MS/MS analysis. The immunoregulation activity of β-glucooligosaccharides was correlated with their DPs and the average ratios of β-(1→3) to β-(1→6)-linked glucose units. F4 fraction with high DPs and ratio of 3.29:1 exhibited higher immunoenhancing activity on inducing NF-κB activation through binding to dectin-1. Surface plasmon resonance (SPR) analysis indicated that β-glucooligosaccharides could bind to Dectin-1 directly and the binding affinity increased with the increase of DPs and the ratios of β-(1→3)-linked glucose.
Ganoderma lucidum, Dectin-1, β-glucooligosaccharide, HPAEC-PAD-MS/MS
Structure type: polymer chemical repeating unit ; 2420000
Location inside paper: p. 1198
Trivial name: water soluble β-D-glucan (GLP20)
Contained glycoepitopes: IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_241101,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: MALDI-TOF MS, HPSEC, cytokine analysis, HPAEC-PAD, extraction, statistical analysis, SPR, immunomodulatory activity analysis, microwave degradation
Comments, role: published polymerization frame was shifted for conformity with other records.
NCBI Taxonomy refs (TaxIDs): 5315
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There is only one chemically distinct structure:
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