Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: conidia,
mycelium
The structure was elucidated in this paperPublication DOI: 10.3390/microbiolres13030028Journal NLM ID: 101535908Publisher: Pavia: PagePress Publications
Correspondence: E. Barreto-Bergter <eliana.bergter
micro.ufrj.br>
Institutions: Laboratório de Química Biológica de Microorganismos, Instituto de Microbiologia, Universidade Federal do Rio de Janeiro (UFRJ), Av. Carlos Chagas Filho, 373, CCS, Rio de Janeiro 21941590, Brazil, Laboratório de Virologia Molecular Vegetal, Departamento de Virologia, Instituto de Microbiologia, Universidade Federal do Rio de Janeiro (UFRJ), Av. Carlos Chagas Filho, 373, CCS, Rio de Janeiro 21941590, Brazil
Cladosporium species are widely distributed filamentous fungi. One of the most important species is C. herbarum, which is related to infections in a variety of plants and of concern in plantations. Fungal cerebrosides, such as glucosylceramide (GlcCer), have been described as playing important roles in fungal growth and pathogenesis, but GlcCer from C. herbarum has not been characterized so far. For this reason, the present study aimed to elucidate the chemical structure of GlcCer from C. herbarum and its role in the interaction with Passiflora edulis. Mass spectrometry characterization of purified GlcCer revealed two major molecular ions, m/z 760 and m/z 774, and it reacts with monoclonal anti-GlcCer antibodies and is exposed on the fungal surface. P. edulis treatment with GlcCer induced increased levels of superoxide as well as the expression of some genes related to plant defense, such as PR3, POD, LOX and PAL. GlcCer also enhanced growth parameters, such as plant height and root weight. All these results suggest that C. herbarum GlcCer can stimulate plant defense mechanisms, which could help plants to face fungal infections.
fungi, glucosylceramides, Cladosporium herbarum, Passiflora edulis, plant defense-related genes
Structure type: monomer ; 760 [M+Li]+
Location inside paper: p. 354, Fig. 3B
Trivial name: glucosylceramide
Compound class: glycolipid
Contained glycoepitopes: IEDB_137339,IEDB_142488,IEDB_146664,IEDB_983931,SB_192,SB_5
Methods: sugar analysis, TLC, ELISA, ESI-MS, acid hydrolysis, extraction, statistical analysis, RT-qPCR, differential interference contrast microscopy, ROS measurement, plant developmental parameter evaluation
Comments, role: the major proposed structure of GlcCer
NCBI Taxonomy refs (TaxIDs): 29918
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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