Taxonomic group: fungi / Basidiomycota, Ascomycota
(Phylum: Basidiomycota, Ascomycota)
Organ / tissue: fruiting bodies
The structure was elucidated in this paperNCBI PubMed ID: 36138494Publication DOI: 10.1093/bbb/zbac157Journal NLM ID: 9205717Publisher: Japan Society for Bioscience, Biotechnology, and Agrochemistry
Correspondence: J. Koga <jinichiro_koga
nasu.bio.teikyo-u.ac.jp>
Institutions: Department of Biosciences, School of Science and Engineering, Teikyo University, Tochigi, Japan, Advanced Instrumental Analysis Center, Teikyo University, Tochigi, Japan
Ingestion of plant and fungal glucosylceramides is known to reduce colon carcinogenesis and skin barrier damage in mice and humans. However, such effects in animal experiments have not been revealed for plant and fungal ceramides because the content of ceramides contained in plants and fungi is so low that the large amount required for animal experiments is difficult to obtain. Noting that the fungus shiitake mushroom (Lentinula edodes) is rich in a glucosylceramide, (4E,8E)-N-d-2'-hydroxypalmitoyl-1-O-β-D-glucopyranosyl-9-methyl-4,8-sphingadienine [Glc-d19:2(4E,8E,9Me)-h16:0], we developed a new method to purify this fungal glucosylceramide using ethanol precipitation and high-performance liquid chromatography. We also developed a new method to produce large amounts of a ceramide [d19:2(4E,8E,9Me)-h16:0] from this purified glucosylceramide using human glycoside hydrolase family 30 glucocerebrosidase (imiglucerase). These methods will be useful for elucidating the physiological function by ingestion of fungal ceramides in animal experiments.
ceramide, glucosylceramide, Lentinula edodes, shiitake mushroom, glucocerebrosidase
Structure type: monomer ; 728.6 [M+H]+
Location inside paper: abstract, Fig. 1(a)
Trivial name: glucosylceramide
Compound class: glycolipid
Contained glycoepitopes: IEDB_137339,IEDB_142488,IEDB_146664,IEDB_983931,SB_192,SB_5
Methods: HPLC, LC-ESI-MS/MS, EtOH extraction, EtOH precipitation, hydrolysis by human GH30 glucocerebrosidase
NCBI Taxonomy refs (TaxIDs): 5353,
318829
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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