Poria cocos, a famous traditional Chinese medicine and a well-known food or food supplement, has shown therapeutic potential against cancer and the uneasiness of the mind. In addition, polysaccharides (PCPs) in this fungus were found to be various bioactive. In this work, one such PCP, PCP-1, extracted by deep eutectic solvent (DES) and separated using Sephadex G-15 columns, was characterized using GC-MS, HPGPC, FT-IR, and NMR, while also tested for physicochemical properties. Results indicated that PCP-1 contained 96.89 ± 3.21% total sugars and was a glucan with molecular weight of 3.2 kD. The main glycosidic linkage was 1,3-linked Glcp with 96.82 mol% content and a triple helix structure, and β-D-Glcp-(1→ linkage connected to the main chain through an O-6 atom was the backbone structure. In terms of the physicochemical property, PCP-1 was soluble in water, but not in organic solvent, and processed a relative high water-holding capacity (8.64 ± 0.14 g/g) and low oil-holding capacity (2.52 ± 0.21 g/g). In addition, in vitro, PCP-1 was found to have the ability of scavenging DPPH, hydroxyl free radical, superoxide anion radical and reducing ferric at different levels. This research would be useful for the further application of PCP-1.

polysaccharide, Structural characterization, Poria cocos

← originally published structure   Show legend

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5	C6
3,3	bDGlcp	103.94	74.77	85.65	69.64	77.04	62.05
3,6	bDGlcp	104.13	72.73	74.56	69.49	76.7	64.00
3	bDGlcp	104.14	74.72	85.6	70.83	77.43	66.88
	bDGlcp


1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5	H6
3,3	bDGlcp	4.72	3.49	3.71	3.43	3.44	3.67-3.85
3,6	bDGlcp	4.67	3.49	3.69	3.76	3.66	3.48-3.58
3	bDGlcp	4.68	3.29	3.47	3.3	3.42	3.72-3.89
	bDGlcp


1H/13C HSQC data:
Linkage	Residue	C1/H1	C2/H2	C3/H3	C4/H4	C5/H5	C6/H6
3,3	bDGlcp	103.94/4.72	74.77/3.49	85.65/3.71	69.64/3.43	77.04/3.44	62.05/3.67-3.85
3,6	bDGlcp	104.13/4.67	72.73/3.49	74.56/3.69	69.49/3.76	76.7/3.66	64.00/3.48-3.58
3	bDGlcp	104.14/4.68	74.72/3.29	85.6/3.47	70.83/3.3	77.43/3.42	66.88/3.72-3.89
	bDGlcp

There is only one chemically distinct structure:

SVGMWSMILES3D molIonize

 

Warning = sub-repeat ending at bDGlcp: large unit count (38) was reduced to 5
[*]O[C@H]1[C@H](O)[C@@H](CO)O[C@@H](O[C@@H]2[C@@H](O)[C@H](O[C@@H]3[C@@H](O)[C@H](O[C@@H]4[C@@H](O)[C@H](O[C@@H]5[C@@H](O)[C@H](O[C@@H]6[C@@H](O)[C@H](O[C@@H]7[C@@H](O)[C@H]([*])O[C@H](CO)[C@H]7O)O[C@H](CO[C@@H]7O[C@H](CO)[C@@H](O)[C@H](O)[C@H]7O)[C@H]6O)O[C@H](CO)[C@H]5O)O[C@H](CO)[C@H]4O)O[C@H](CO)[C@H]3O)O[C@H](CO)[C@H]2O)[C@@H]1O

1297.128 g/mol (C₄₈H₈₀R₂O₄₀, R = next and previous repeats, not counted in mol weight)