Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: basidiocarp of polypore
The structure was elucidated in this paperNCBI PubMed ID: 35054662Publication DOI: 10.3390/polym14020255Journal NLM ID: 101545357Publisher: Basel: MDPI
Correspondence: R. Bleha <blehar
vscht.cz >; A. Synytsya <sinicaa
vscht.cz>
Institutions: Department of Carbohydrates and Cereals, UCT Prague, 166 28 Prague, Czech Republic, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 842 38 Bratislava, Slovakia, Department of Gardening, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, 165 00 Prague, Czech Republic, Department of Crop Production, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, 165 00 Prague, Czech Republic
In this study, we focused on the isolation and structural characterization of polysaccharides from a basidiocarp of polypore fungus Ganoderma resinaceum. Polysaccharide fractions were obtained by successive extractions with cold water at room temperature (20 °C), hot water under reflux (100 °C), and a solution of 1 mol L-1 sodium hydroxide. The purity of all fractions was controlled mainly by Fourier transform infrared (FTIR) spectroscopy, and their composition and structure were characterized by organic elemental analysis; neutral sugar and methylation analyses by gas chromatography equipped with flame ionization detector (GC/FID) and mass spectrometry detector (GC/MS), respectively; and by correlation nuclear magnetic resonance (NMR) spectroscopy. The aqueous extracts contained two main polysaccharides identified as a branched O-2-β-D-mannosyl-(1→6)-α-D-galactan and a highly branched (1→3)(1→4)(1→6)-β-D-glucan. Mannogalactan predominated in the cold water extract, and β-D-glucan was the main product of the hot water extract. The hot water soluble fraction was further separated by preparative anion exchange chromatography into three sub-fractions; two of them were identified as branched β-D-glucans with a structure similar to the corresponding polysaccharide of the original fraction. The alkaline extract contained a linear (1→3)-α-D-glucan and a weakly branched (1→3)-β-D-glucan having terminal β-D-glucosyl residues attached to O-6 of the backbone. The insoluble part after all extractions was identified as a polysaccharide complex containing chitin and β-D-glucans.
polysaccharides, spectroscopy, Ganoderma resinaceum, wood decay fungi
Structure type: fragment of a bigger structure
Location inside paper: table 6, water soluble fraction F1
Trivial name: α-D-glucan, glycogen, amylose-like α-D-glucan
Compound class: EPS, cell wall polysaccharide
Contained glycoepitopes: IEDB_140629,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_420417,IEDB_420418,IEDB_420421,IEDB_857742,IEDB_983931,SB_192
Methods: GC-MS, sugar analysis, GC, FTIR, extraction, elemental analysis, anion exchange chromatography, FT Raman spectroscopy
Comments, role: amylose-like α-D-glucan (fragment) is the minor product of water soluble fraction F1; NMR temperature: 293 or 353
Related record ID(s): 51603, 51708, 51709, 51710
NCBI Taxonomy refs (TaxIDs): 34465Reference(s) to other database(s): GTC:G05740LL
Show glycosyltransferases
NMR conditions: in D2O at 323 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
aDGlcp 100.3 71.5 73.3 78.8 72.6 60.7
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
aDGlcp 5.34 3.60 3.92 3.53 3.80 3.87
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
aDGlcp 100.3/5.34 71.5/3.60 73.3/3.92 78.8/3.53 72.6/3.80 60.7/3.87
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| | aDGlcp | 5.34 | 3.60 | 3.92 | 3.53 | 3.80 | 3.87 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | aDGlcp | 100.3 | 71.5 | 73.3 | 78.8 | 72.6 | 60.7 |
|
There is only one chemically distinct structure:
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