Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: diarrhea [ICD11:
ME05.1 
, ICD11:
SA55 ]
The structure was elucidated in this paperNCBI PubMed ID: 31622726Publication DOI: 10.1016/j.ijbiomac.2019.09.135Journal NLM ID: 101092791Publisher: Basel, Switzerland: MDPI
Correspondence: Yanwen Xiong <xiongyanwen
icdc.cn>
Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Zigong Center for Disease Control and Prevention, Zigong, Sichuan Province, China, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, Zhejiang Province, China, State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China, Anhui Provincial Center for Disease Control and Prevention, Hefei, China, Division I of Risk Assessment, China National Center for Food Safety Risk Assessment, Beijing, China
O antigen is a polysaccharide chain of a lipopolysaccharide on the outer membrane of Gram-negative bacteria. O-antigen-based serotyping and molecular typing are widely used for epidemiological and surveillance purposes. Two polysaccharides were isolated by Sephadex G-50 gel-permeation chromatography following mild acid degradation of the lipopolysaccharide of Escherichia albertii EA046 assigned to serotype O9. The polysaccharide eluted first was considered as the O-antigen. It was composed of tetrasaccharide repeating units containing two residues of d-Man and one residue each of d-Gal and d-GlcNAc as well as glycerol phosphate. It had the following unique structure which was established by NMR spectroscopy applied to the initial and dephosphorylated polysaccharides: The polysaccharide eluted from the gel second was identified as a mannan with a → 3)-β-d-Manp-(1 → 2)-α-d-Manp-(1 → 2)-α-d-Manp-(1 → trisaccharide repeating unit. In E. albertii EA046, two polysaccharide gene clusters were found at a chromosomal locus flanked by the conserved galF gene and the histidine synthesis operon (his). They were suggested to drive the biosynthesis of the O-antigen by the Wzy/Wzy-dependent pathway and the mannan by the Wzm/Wzt-dependent pathway. The mannan shares the structure and gene cluster with a polysaccharide isolated earlier from the lipopolysaccharide of Escherichia coli O8.
Lipopolysaccharide, O-antigen, Escherichia albertii
Structure type: polymer chemical repeating unit
Location inside paper: abstract, table 1, p. 612, Fig. 3, PS2
Trivial name: mannan
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_137485,IEDB_140116,IEDB_142357,IEDB_143632,IEDB_144983,IEDB_144995,IEDB_152206,IEDB_164479,IEDB_2278480,IEDB_983930,SB_136,SB_196,SB_197,SB_44,SB_67,SB_72
Methods: 13C NMR, 1H NMR, NMR-2D, DNA sequencing, sugar analysis, dephosphorylation, 31P NMR, GLC, GPC, function analysis of gene clusters
Related record ID(s): 5182, 32169
NCBI Taxonomy refs (TaxIDs): 208962Reference(s) to other database(s): GTC:G40440IC, GlycomeDB:
27903
Show glycosyltransferases
NMR conditions: in D2O at 310 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
2,2 bDManp 101.4 72.0 81.6 68.3 77.6 62.3
2 aDManp 99.7 79.9 71.5 67.5 74.8 61.9
aDManp 102.1 78.4 71.1 68.3 74.7 62.7
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
2,2 bDManp 5.14 4.16 3.72 3.70 3.41 3.75-3.90
2 aDManp 4.79 4.11 4.00 3.69 3.75 3.78-3.82
aDManp 5.34 4.28 3.86 3.71 3.72 3.77-3.80
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
2,2 bDManp 101.4/5.14 72.0/4.16 81.6/3.72 68.3/3.70 77.6/3.41 62.3/3.75-3.90
2 aDManp 99.7/4.79 79.9/4.11 71.5/4.00 67.5/3.69 74.8/3.75 61.9/3.78-3.82
aDManp 102.1/5.34 78.4/4.28 71.1/3.86 68.3/3.71 74.7/3.72 62.7/3.77-3.80
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 2,2 | bDManp | 5.14 | 4.16 | 3.72 | 3.70 | 3.41 | 3.75
3.90 |
| 2 | aDManp | 4.79 | 4.11 | 4.00 | 3.69 | 3.75 | 3.78
3.82 |
| | aDManp | 5.34 | 4.28 | 3.86 | 3.71 | 3.72 | 3.77
3.80 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 2,2 | bDManp | 101.4 | 72.0 | 81.6 | 68.3 | 77.6 | 62.3 |
| 2 | aDManp | 99.7 | 79.9 | 71.5 | 67.5 | 74.8 | 61.9 |
| | aDManp | 102.1 | 78.4 | 71.1 | 68.3 | 74.7 | 62.7 |
|
There is only one chemically distinct structure:
Found 
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