Oligosaccharides formed by a transgalactosylation reaction during lactose hydrolysis with Bifidobacterium bifidum were separated into eight fractions by gel-permeation chromatography and their structures studies determined by trimethylsilylation analysis, methylation analysis, f.a.b.—m.s., g.l.c.—m.s. and enzymic hydrolysis as β-d-Galp-(1→3)-d-Glc, β-d-Galp-(1→6)-d-Glc, β-d-Galp-(1→6)-d-Glc, β-d-Galp-(1→3)-β-d-Galp-(1→4)-d-Glc, β-d-Galp-(1→6)[β-d-Galp-(1→4)]-d-Glc, β-d-Galp-(1→2)[β-d-Galp-(1→6)]-d-Glc, β-d-Galp-(1→3)-β-d-Galp-(1→3)-β-d-Galp-(1→4)-d-Glc, β-d-Galp-(1→3)-β-d-Galp-(1→3)-β-d-Galp-(1→3)-β-d-Galp-(1→4)-d-Glc, β-d-Galp-(1→3)-β-d-Galp-(1→3)-β-dGalp-(1→3)-β-d-Galp-(1→3)-β-d-Galp-(1→4)-d-Glc, and β-d-Galp-(1→3)-β-d-Galp-(1→3)-β-d-Galp-(1→3)-β-d-Galp-(1→3)-β-d-Galp-(1→3) β-d-Galp-(1→4)-d-Glc.
NCBI PubMed ID: 2119886Publication DOI: 10.1016/0008-6215(90)84228-MJournal NLM ID: 0043535Publisher: Elsevier
Institutions: Laboratoire de Chimie Biologique, Unité Mixte du C.N.R.S. No. 111, Université des Sciences et Techniques de Lille Flandres-Artois, F 59655 Villeneuve d'Ascq France
Methods: GLC-MS, FAB-MS, enzymatic hydrolysis
Found