The Staphylococcus aureus type 5 capsular polysaccharide is composed of 2-acetamido-2-deoxy-l-fucose (1 part), 2-acetamido-2-deoxy-d-fucose (1 part), and 2-acetamido-2-deoxy-d-mannuronic acid (1 part). On the basis of methylation analysis, optical rotation, high-field one- and two-dimensional 1H- and 13C-n.m.r. experiments, and selective cleavage with 70% aqueous hydrogen fluoride, the polysaccharide was found to be a partially O-acetylated (50%) polymer of the repeating trisaccharide unit, [→4)-3-O-Ac-β-d-ManpNAcA-(1→4)-α-l-FucpNAc-(1→3)-β-d-FucpNAc-(1→]n.
NCBI PubMed ID: 2224883Publication DOI: 10.1016/0008-6215(90)84244-OJournal NLM ID: 0043535Publisher: Elsevier
Institutions: Office of Biologics Research and Review, Food and Drug Administration, Bethesda, MD, USA, U.S.A., Division of Biological Sciences, National Research Council of Canada, Ottawa K1A OR6 Canada, Unité du Cholerá et des Vibrions, Institut Pasteur, F-75724 Paris France
Methods: gel filtration, 13C NMR, 1H NMR, methylation, GLC-MS, GLC, HF treatment
Found