CSDB: Search results

Taxonomic group: bacteria / Firmicutes (Phylum: Firmicutes)
Associated disease: infection due to Streptococcus pneumoniae [ICD11: XN3PW

]

NCBI PubMed ID: 14573636
Journal NLM ID: 0246127
Publisher: American Society for Microbiology
Correspondence: j.vanputten

vet.uu.nl
Institutions: Department of Infectious Diseases and Immunology, Utrecht University, 3584 CL Utrecht, Genencor International B.V., 2300 AE Leiden and Netherlands Vaccine Institute, 3720 AL Bilthoven, The Netherlands, Genencor International B.V., 2300 AE Leiden and Netherlands Vaccine Institute, 3720 AL Bilthoven, The Netherlands.

In a search for the genetic basis for the structural difference between the related Streptococcus pneumoniae capsular serotypes 15B and 15C and for the reported reversible switching between these serotypes, the corresponding capsular polysaccharide synthesis (cps) loci were investigated by keeping in mind that at the structural level, the capsules differ only in O acetylation. The cps locus of a serotype 15B strain was identified, partially PCR amplified with primers based on the related serotype 14 sequence, and sequenced. Sequence analysis revealed, among other open reading frames, an intact open reading frame (designated cps15bM) whose product, at the protein level, exhibited characteristics of previously identified acetyltransferases. Genetic analysis of the corresponding region in a serotype15C strain indicated that the same gene was present but had a premature stop in translation. Closer analysis indicated that the serotype 15B gene contained a short tandem TA repeat consisting of eight TA units. In serotype 15C, this gene contained nine TA units that resulted in a frameshift and a truncated product. Genetic analysis of 17 serotype 15B and 15C clinical isolates revealed a perfect correlation between the serotype and the length of the short tandem repeat in the putative O-acetyltransferase gene. The number of TA repeating units varied between seven and nine in the various isolates. Together, the data strongly suggest that the structural difference between serotypes 15B and 15C is based on variation in the short tandem TA repeat in the O-acetyltransferase gene and that the transition between serotypes is due to slipped-strand mispairing with deletion or insertion of TA units in the cps15bM gene.

genetic, structural, capsular, polysaccharide, serotype, Streptococcus, Streptococcus pneumoniae, capsular polysaccharides, capsular polysaccharide, polysaccharides, difference

Structure type: polymer chemical repeating unit
Location inside paper: fig. 1, p.6193
The structure in this paper was incorrect:

Original structure from CSDB record 5272

← originally published structure

Show legend

Compound class: CPS
Contained glycoepitopes: IEDB_130646,IEDB_130697,IEDB_131186,IEDB_135813,IEDB_135818,IEDB_136044,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_137776,IEDB_1391966,IEDB_1392542,IEDB_140108,IEDB_140110,IEDB_140122,IEDB_141794,IEDB_141807,IEDB_142351,IEDB_142487,IEDB_142488,IEDB_143634,IEDB_146664,IEDB_149138,IEDB_149139,IEDB_149141,IEDB_149142,IEDB_149143,IEDB_149144,IEDB_149145,IEDB_149147,IEDB_149148,IEDB_149149,IEDB_149150,IEDB_149151,IEDB_151528,IEDB_151531,IEDB_167072,IEDB_190606,IEDB_742245,IEDB_983931,SB_145,SB_165,SB_166,SB_173,SB_187,SB_192,SB_195,SB_30,SB_36,SB_6,SB_7,SB_88

Biosynthesis and genetic data: genetic data
Comments, role: the structure was revised in ID 10063

Related record ID(s): 5348
NCBI Taxonomy refs (TaxIDs): 1313
Reference(s) to other database(s): GTC:G85939RW, GlycomeDB:5547
Show glycosyltransferases

There is only one chemically distinct structure:

*OC[C@H]1O[C@@H](O[C@H]2[C@@H](O)[C@@H](CO)O[C@@H](O[C@@H]3[C@@H](CO)O[C@@H](*)[C@H](O)[C@H]3O)[C@@H]2O)[C@H](NC(C)=O)[C@@H](O)[C@@H]1O[C@@H]1O[C@H](CO)[C@H](O)[C@H](OP(=O)(O)OC(CO)CO)[C@H]1O[C@H]1O[C@H](COC(C)=O)[C@H](OC(C)=O)[C@H](OC(C)=O)[C@H]1OC(C)=O

SVG MW SMILES 3D mol Ionize