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1. (Article ID: 5426)
 
Dworaczek K, Kurzylewska M, Karaś MA, Janczarek M, Pękala-Safińska A, Turska-Szewczuk A
A Unique Sugar l-Perosamine (4-Amino-4,6-dideoxy-l-mannose) Is a Compound Building Two O-Chain Polysaccharides in the Lipopolysaccharide of Aeromonas hydrophila Strain JCM 3968, Serogroup O6
Marine Drugs 17(5) (2019) E254
 

Lipopolysaccharide (LPS) is the major glycolipid and virulence factor of Gram-negative bacteria, including Aeromonas spp. The O-specific polysaccharide (O-PS, O-chain, O-antigen), i.e., the surface-exposed part of LPS, which is a hetero- or homopolysaccharide, determines the serospecificity of bacterial strains. Here, chemical analyses, mass spectrometry, and 1H and 13C NMR spectroscopy techniques were employed to study the O-PS of Aeromonas hydrophila strain JCM 3968, serogroup O6. MALDI-TOF mass spectrometry revealed that the LPS of A. hydrophila JCM 3968 has a hexaacylated lipid A with conserved architecture of the backbone and a core oligosaccharide composed of Hep6Hex1HexN1HexNAc1Kdo1P1. To liberate the O-antigen, LPS was subjected to mild acid hydrolysis followed by gel-permeation-chromatography and revealed two O-polysaccharides that were found to contain a unique sugar 4-amino-4,6-dideoxy-l-mannose (N-acetyl-l-perosamine, l-Rhap4NAc), which may further determine the specificity of the serogroup. The first O-polysaccharide (O-PS1) was built up of trisaccharide repeating units composed of one α-d-GalpNAc and two α-l-Rhap4NAc residues, whereas the other one, O-PS2, is an α1→2 linked homopolymer of l-Rhap4NAc. The following structures of the O-polysaccharides were established: O-PS1 →3)-α-l-Rhap4NAc-(1→4)-α-d-GalpNAc-(1→3)-α-l-Rhap4NAc-(1→, O-PS2 →2)-α-l-Rhap4NAc-(1→. The present paper is the first work that reveals the occurrence of perosamine in the l-configuration as a component of bacterial O-chain polysaccharides.

O-antigen, NMR spectroscopy, O-polysaccharide, endotoxin, structure elucidation, lipopolysaccharide (LPS), MALDI-TOF mass spectrometry, Aeromonas hydrophila, L-perosamine, l-Rha4NAc

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2. (Article ID: 6283)
 
Nguyen JM, Evans CS, Wright NM, Townsend SD
Synthesis of the Aeromonas veronii strain Bs8 disaccharide repeating unit
Carbohydrate Research 514 (2022) 108530
 

Presented herein is the synthesis of the Aeromonas veronii disaccharide repeating unit which has been achieved in 11 steps starting from D-fucose and D-galactosamine.

synthesis, Gram-negative, fucose, glycosylation, GalNAc

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