A new tetrasaccharide derivative, actinotetraose L (1), was isolated from the methanol extract of the mycelia of the grasshopper-associated rare actinobacterium Amycolatopsis sp. HCa1. The structure of the new compound was elucidated by a combination of 1D, 2D NMR (HMQC, HMBC, COSY, and NOESY), and HR-ESI-MS analyses as O-{3,4-di-O-[(E)-2-ethyl-2-butenoyl]-β-D-glucopyranosyl-(1→2)}-O-{3,4-di-O-[(E)-2-methyl-2-butenoyl]-β-D-glucopyranosyl-(1→2)}-6,6-di-O-[(E)-2-methyl-2-butenoyl]-α-D-glucopyranosyl-(1→1)-α-D-glucopyranoside. The immunosuppressive activity and cytotoxicity of the compound were evaluated by T-cell viability and MTT assays. Nonetheless, no significant activity was observed

tetrasaccharide, Amycolatopsis, Pseudonocardiaceae, grasshopper

• Compound ID: 14291
  

  Subst-(1-4)-+ | Subst-(1-3)-b-D-Glcp-(1-2)-+ | Tig-(1-4)-+ Tig-(1-6)-+ | | | | Tig-(1-3)-b-D-Glcp-(1-2)-a-D-Glcp-(1-1)-a-D-Glcp | Tig-(1-6)-+ Subst = (E)-2-ethylbut-2-enoic acid = SMILES C/C=C(CC)/{1}C(=O)O

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  Structure type: oligomer ; 1209.4941 [M+Na]+
  C56H82O27
  Trivial name: actinotetraose L
  Compound class: glycolipid
  
   
  
  Amycolatopsis sp. HCa1
  CSDB ID 47840 (all data & tools)