Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Klebsiella pneumoniae [ICD11:
XN741 
]
The structure was elucidated in this paperNCBI PubMed ID: 11986326Journal NLM ID: 2985121RPublisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Correspondence: evguenii.vinogradov
nrc.ca
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, Ontario K1A 0R6, Canada, Department of Microbiology, University of Guelph, Guelph, Ontario N1G 2W1, Canada, Carlsberg Laboratory, Department of Chemistry, Gamle Carlsberg Vej 10, DK-2500 Valby, Copenhagen, Denmark
Deamination of LPSs from Klebsiella pneumoniae released O-chain polysaccharides together with a fragment of the core oligosaccharide. The structures of the products from serotypes O1, O2a, O2a,c, O3, O4, O5, and O12 were determined by NMR spectroscopy and chemical methods, identifying the linkage region between the O antigens and the core as well as novel residues at the non-reducing ends of the polysaccharides. All serotypes had an identical linkage between the O chain and core.
lipopolysaccharides, oligosaccharide, structure, core oligosaccharide, NMR spectroscopy, O-antigens, linkage, region, O-chain, Klebsiella pneumoniae, reducing
Structure type: polymer chemical repeating unit
Location inside paper: fig.2
Trivial name: mannose homopolysaccharide
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_115576,IEDB_130701,IEDB_136104,IEDB_140116,IEDB_141111,IEDB_141795,IEDB_141830,IEDB_143632,IEDB_144983,IEDB_152206,IEDB_153756,IEDB_164174,IEDB_164175,IEDB_164176,IEDB_164480,IEDB_174840,IEDB_241100,IEDB_76933,IEDB_983930,SB_136,SB_196,SB_197,SB_44,SB_67,SB_72
Methods: NMR-2D, NMR, serological methods, genetic methods
Biological activity: serological data
Biosynthesis and genetic data: genetic data
Comments, role: published polymerization frame was shifted for conformity with other records.
Related record ID(s): 883, 5513, 5751, 5752, 5753, 5755, 5756, 5757, 5758, 5759, 5760, 5761, 5763, 5764, 5765, 5766, 5767, 7199, 10527, 20624, 25215
NCBI Taxonomy refs (TaxIDs): 573Reference(s) to other database(s): GTC:G87424QV, GlycomeDB:
668, CCSD:
47538
Show glycosyltransferases
NMR conditions: in D2O at 313 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
2,2,2,3 aDManp 103.0 70.7 79.4 67.2 74.4 61.9
2,2,2 aDManp 103.0 70.6 79.0 67.2 74.3 61.9
2,2 aDManp 101.6 79.4 71.1 68.0 74.2 62.1
2 aDManp 101.6 79.6 71.1 67.9 74.2 62.0
aDManp 101.6 79.5 71.1 68.1 74.3 62.1
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
2,2,2,3 aDManp 5.05 4.15 3.92 3.70 3.75 3.69-3.83
2,2,2 aDManp 4.97 4.15 3.87 3.70 3.72 3.69-3.81
2,2 aDManp 5.22 4.04 3.87 3.63 3.67 3.69-3.80
2 aDManp 5.21 4.02 3.88 3.64 3.67 3.70-3.79
aDManp 5.29 4.01 3.92 3.62 3.72 3.69-3.84
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
2,2,2,3 aDManp 103.0/5.05 70.7/4.15 79.4/3.92 67.2/3.70 74.4/3.75 61.9/3.69-3.83
2,2,2 aDManp 103.0/4.97 70.6/4.15 79.0/3.87 67.2/3.70 74.3/3.72 61.9/3.69-3.81
2,2 aDManp 101.6/5.22 79.4/4.04 71.1/3.87 68.0/3.63 74.2/3.67 62.1/3.69-3.80
2 aDManp 101.6/5.21 79.6/4.02 71.1/3.88 67.9/3.64 74.2/3.67 62.0/3.70-3.79
aDManp 101.6/5.29 79.5/4.01 71.1/3.92 68.1/3.62 74.3/3.72 62.1/3.69-3.84
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 2,2,2,3 | aDManp | 5.05 | 4.15 | 3.92 | 3.70 | 3.75 | 3.69
3.83 |
| 2,2,2 | aDManp | 4.97 | 4.15 | 3.87 | 3.70 | 3.72 | 3.69
3.81 |
| 2,2 | aDManp | 5.22 | 4.04 | 3.87 | 3.63 | 3.67 | 3.69
3.80 |
| 2 | aDManp | 5.21 | 4.02 | 3.88 | 3.64 | 3.67 | 3.70
3.79 |
| | aDManp | 5.29 | 4.01 | 3.92 | 3.62 | 3.72 | 3.69
3.84 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 2,2,2,3 | aDManp | 103.0 | 70.7 | 79.4 | 67.2 | 74.4 | 61.9 |
| 2,2,2 | aDManp | 103.0 | 70.6 | 79.0 | 67.2 | 74.3 | 61.9 |
| 2,2 | aDManp | 101.6 | 79.4 | 71.1 | 68.0 | 74.2 | 62.1 |
| 2 | aDManp | 101.6 | 79.6 | 71.1 | 67.9 | 74.2 | 62.0 |
| | aDManp | 101.6 | 79.5 | 71.1 | 68.1 | 74.3 | 62.1 |
|
There is only one chemically distinct structure:
Found 
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