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1. (Article ID: 5762)
 
Dworaczek K, Kurzylewska M, Laban M, Pękala-Safińska A, Marczak M, Turska-Szewczuk A
Structure of the disaccharide repeating unit of O-specific polysaccharide isolated from Aeromonas veronii strain Bs8 pathogenic to common carp (Cyprinus carpio)
Carbohydrate Research 500 (2021) 108210
 

The O-specific polysaccharide (OPS) was isolated from the lipopolysaccharide of Aeromonas veronii strain Bs8, which is pathogenic to common carp (Cyprinus carpio), after mild acid hydrolysis followed by gel-permeation chromatography. The high-molecular-mass OPS fraction was investigated using chemical methods, mass spectrometry, and 1H and 13C NMR spectroscopy techniques, including 2D homonuclear 1H,1H TOCSY, DQF COSY, NOESY, and heteronuclear 1H-detected 1H,13C HSQC, and HMBC experiments. The analysis revealed that the O-specific polysaccharide contains sugars with the galacto configuration of the ring and is composed of a disaccharide repeating unit with the following structure. (formula: see text).

LPS, O-antigen, O-specific polysaccharide, Aeromonas veronii, Fish pathogen, D-fucopyranose, D-Fucp, GalpNAc

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