Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: nosocomial infections [ICD11:
XB25 
];
infection due to Acinetobacter baumannii [ICD11:
XN8LS ]
NCBI PubMed ID: 34073255Publication DOI: 10.3390/ijms22115641Journal NLM ID: 101092791Publisher: Basel, Switzerland: MDPI
Correspondence: A.O. Chizhov <chizhov
ioc.ac.ru>
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia, Centre for Immunology and Infection Control, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, QLD 4059, Australia, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia, State Research Center for Applied Microbiology and Biotechnology, Obolensk, 142279 Moscow Region, Russia
Whole genome sequences of two Acinetobacter baumannii clinical isolates, 48-1789 and MAR24, revealed that they carry the KL106 and KL112 capsular polysaccharide (CPS) biosynthesis gene clusters, respectively, at the chromosomal K locus. The KL106 and KL112 gene clusters are related to the previously described KL11 and KL83 gene clusters, sharing genes for the synthesis of l-rhamnose (l-Rhap) and 6-deoxy-l-talose (l-6dTalp). CPS material isolated from 48-1789 and MAR24 was studied by sugar analysis and Smith degradation along with one- and two-dimensional 1H and 13C NMR spectroscopy. The structures of K106 and K112 oligosaccharide repeats (K units) l-6dTalp-(1→3)-D-GlcpNAc tetrasaccharide fragment share the responsible genes in the respective gene clusters. The K106 and K83 CPSs also have the same linkage between K units. The KL112 cluster includes an additional glycosyltransferase gene, Gtr183, and the K112 unit includes α l-Rhap side chain that is not found in the K106 structure. K112 further differs in the linkage between K units formed by the Wzy polymerase, and a different wzy gene is found in KL112. However, though both KL106 and KL112 share the atr8 acetyltransferase gene with KL83, only K83 is acetylated.
capsular polysaccharide, Acinetobacter baumannii, 6-deoxy-L-talose, K locus, K106, K112
Structure type: polymer chemical repeating unit
Location inside paper: Fig. 1, K83
Trivial name: K83 CPS
Compound class: CPS
Contained glycoepitopes: IEDB_135813,IEDB_136105,IEDB_137340,IEDB_141807,IEDB_142488,IEDB_146664,IEDB_151531,IEDB_225177,IEDB_885823,IEDB_983931,SB_192,SB_74,SB_85
Methods: 13C NMR, 1H NMR, NMR-2D, GLC, Smith degradation, composition analysis, GPC, bioinformatic analysis, HR-ESI-MS, sequencing
Enzymes that release or process the structure: Wzy(K83),Gtr154, Gtr27, Gtr60, Atr8,Gtr29, Wzy(K83)[ItrA3]
Related record ID(s): 5840, 5841, 5842, 5843, 5844, 10873
NCBI Taxonomy refs (TaxIDs): 470Reference(s) to other database(s): GTC:G20936VX
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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